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  4. Mouse C-Reactive Protein,CRP ELISA Kit

Mouse C-Reactive Protein,CRP ELISA Kit

  • 中文名稱：
    小鼠C反應蛋白(CRP)酶聯免疫試劑盒
  • 貨號：
    CSB-E07923m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠C反應蛋白(CRP)酶聯免疫試劑盒（CSB-E07923m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CRP含量。CRP即C反應蛋白，是一種急性時相反應蛋白。在炎癥、感染、組織損傷等情況下其水平會迅速升高。研究機制方面，它可與病原體結合，激活補體系統，在免疫防御中發揮作用，也是臨床常用的炎癥和疾病活動監測指標。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，常用于評估機體免疫狀態和炎癥進程；特別適用于實驗動物模型中炎癥相關疾病研究、藥物抗炎效果評價以及免疫調節機制探索等科研領域；可為心血管疾病、代謝綜合征、感染性疾病等基礎研究提供可靠的實驗數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Crp ELISA Kit; Ptx1C-reactive protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %90
    Range %86-93
    1:40Average %103
    Range %99-106
    1:80Average %93
    Range %87-96
    1:160Average %98
    Range %93-101
  • 回收率：
    The recovery of mouse CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9790-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.206 2.124 2.165 1.995
    5001.661 1.655 1.658 1.488
    2501.138 1.122 1.130 0.960
    1250.727 0.721 0.724 0.554
    62.50.424 0.435 0.430 0.260
    31.20.292 0.305 0.299 0.129
    15.60.228 0.234 0.231 0.061
    00.168 0.172 0.170 　
  • 本試劑盒所含材料：
    • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
    • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
    • Biotin-labeled CRPantibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
    • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
    • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
    • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
    • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Seal the microtiter plate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 3 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：1:100

產品評價： 我用CSB-E07923m檢測炎癥血清，樣本的OD值0.8-0.9，測了不同組，結果較為準確，標品操作手冊清楚明了。

By 賈老師

樣品類型：血清

樣品信息：小鼠

稀釋比：1:100

產品評價： 我用CSB-E07923m測關節炎小鼠血清OD值在0.72左右，標準曲線為y=0.00115x+0.10743,r2=0.99309,結果精準靈敏

By 賈老師

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:10

產品評價： 我用CSB-E07923m檢測正常血清，標準曲線OD值為0.105，0.176，0.243，0.398，0.59，0.965，1.438，2.096，樣本的OD值為0.7-1.8，產品的靈敏度和容錯率都很高，操作有點失誤也能測出較好的值

By 萬老師

靶點詳情

  • 功能：
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 基因功能參考文獻：
    1. CRP is pathogenic in type-2 diabetes (T2DN). CRP may promote CD32b- NF-kappaB signaling to mediate renal inflammation; whereas, CRP may enhance renal fibrosis in T2DN via CD32b-Smad3-mTOR signaling. PMID: 27221338
    2. FcgammaRIIb and FcgammaRIII are involved in the CRP-induced expression of MMPs and TIMP-1 and the CRP-induced phosphorylation of p38, whereas the FcgammaR-independent pathway may regulate the CRP-induced MMP-11 expression and the CRP-induced ERK1/2 phosphorylation. PMID: 28539825
    3. C-reactive protein may promote acute kidney injury by impairing tubular epithelial cell regeneration via the CD32-Smad3-p27-driven inhibition of the CDK2/cyclin E complex. PMID: 27470679
    4. These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
    5. these data unexpectedly identify CBS as the major recognition site of mCRP and suggest that this motif may be exploited to tune the proinflammatory actions of mCRP. PMID: 26907682
    6. CRP decreased adiponectin expression and multimerization, while CRP-induced decline in adiponectin might be mediated through the PI3K/Akt pathway. PMID: 26812237
    7. mCRP may be responsible for promoting dementia after ischaemia and mCRP clearance could inform therapeutic avenues to reduce the risk of future dementia. PMID: 26335098
    8. Effects of C-Reactive Protein on the Cardiac Differentiation of Mouse Embryonic Stem Cells PMID: 26328491
    9. These data indicated that CRP may have a direct role on osteoclast and osteoblast differentiation via toll-like receptor signaling pathways. PMID: 26687448
    10. Data suggest that serum amyloid P (SAP) activates CD209 DC-SIGN to regulate the innate immune system differently from C-reactive protein (CRP), and that DC-SIGN is a target for antifibrotics. PMID: 26106150
    11. the acute phase protein C-reactive protein (CRP), a ligand for Fc receptors on phagocytes, enhances antibody-mediated platelet destruction by phagocytes in vivo PMID: 25548320
    12. CRP has therapeutic potential against cancer through decreasing the accumulation of M2 macrophages and angiogenesis within tumors. PMID: 25088786
    13. Orphan nuclear receptor Errgamma induces C-reactive protein gene expression through induction of ER-bound Bzip transmembrane transcription factor CREBH. PMID: 24466039
    14. The interactions of CRP and the BBB are a two-phase process, with increased paracellular permeability at a high dose that enables its entry into the CNS and serves to induce reactive gliosis and impair CNS function. PMID: 23018453
    15. In mice with photochemically induced thrombosis, acrolein produced at the locus of infarction increased the level of IL-6 and then CRP in plasma. PMID: 23763486
    16. CRP directly inhibits tumoral lymphangiogenesis and lymph node metastasis in mice. PMID: 23859309
    17. Which processes in glucose homeostasis are altered by CRP? Data suggest that CRP attenuates insulin-induced skeletal muscle blood flow; CRP contributes to insulin resistance via IgG receptor IIB-mediated inhibition of glucose delivery to muscle. PMID: 23069625
    18. CRP is not only a biomarker, but also a mediator in DKD. PMID: 21744073
    19. C-reactive protein promotes acute renal inflammation and fibrosis in unilateral ureteral obstructive nephropathy in mice. PMID: 21383672
    20. These findings reveal a novel nutrient-dependent interaction between Sirt1 and HNF-1alpha which results in regulation of Crp expression. PMID: 21176092
    21. Studies show that CRP itself may have a pathogenic role in the development of pressure overload-induced cardiac remodeling, possibly through enhanced inflammation and oxidative stress. PMID: 21220701
    22. No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice. PMID: 21149301
    23. Modified and natural C-reactive protein have opposite effects on atherosclerosis in apolipoprotein E-deficient mice. PMID: 16087790
    24. the contribution of CRP's ability of activating the classical pathway of complement in the protection of mice from lethal infection with virulent Streptococcus pneumoniae type 3 PMID: 16547275
    25. nCRP can facilitate cardiovascular disease through impairment of endothelium-dependent vasoreactivity, in a manner that involves increased iNOS activity and a potential for increased peroxynitrite formation. PMID: 17669410
    26. the response to vascular injury provoked by human C-Reactive Protein in ovariectomized C-reactive protein transgenic mice depends on Fc gamma RI and probably requires its expression by F4/80+ cells PMID: 18063701
    27. In vivo analyses revealed significant increases in TF expression and decreases in TFPI expression in carotid arteries of CRP-Tg mice versus WT mice. PMID: 18276908
    28. CRP plays a crucial role in the induction, amplification, and prolongation of inflammatory processes, including atherosclerotic lesions PMID: 18402807
    29. C-reactive protein has a role in impaired endothelial function PMID: 18433756

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Pentraxin family
  • 組織特異性：
    Found in plasma.
  • 數據庫鏈接：


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