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  4. Mouse Beta-klotho(KLB) ELISA kit

Mouse Beta-klotho(KLB) ELISA kit

  • 中文名稱：
    小鼠β-klotho(KLB)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012378MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠β-klotho(KLB)酶聯免疫試劑盒（CSB-EL012378MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KLB含量。KLB（β-Klotho）是一種跨膜蛋白，主要在肝臟和脂肪組織中表達，與FGF19結合后成為FGFR1c和FGFR4的高親和力受體，調節膽汁酸合成和肝細胞增殖。KLB在多種代謝過程中發揮調節作用，其變異與NAFLD兒童肝損害有關，可能對肝細胞的脂毒性和炎癥起保護作用。研究機制涉及FGF19-KLB-FGFRs信號通路，對理解相關疾病的發生發展具有重要意義。試劑盒檢測范圍為2.74 pg/mL-2000 pg/mL，可應用于代謝調控機制研究、基因敲除/過表達模型中KLB蛋白表達驗證，以及藥物干預或營養狀態改變后體內KLB水平動態監測等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Klb ELISA Kit; BetaklBeta-klotho ELISA Kit; BKL ELISA Kit; BetaKlotho ELISA Kit; Klotho beta-like protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2.74 pg/mL-2000 pg/mL
  • 靈敏度：
    2.74 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    86

    Range %

    80-95

    1:2

    Average %

    97

    Range %

    91-102

    1:4

    Average %

    96

    Range %

    92-100

    1:8

    Average %

    91

    Range %

    86-98

  • 回收率：

    The recovery of mouse KLB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    88-98

    EDTA plasma (n=4)

    97

    91-105

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    1.852

    1.956

    1.904

    1.740

    666.67

    1.592

    1.610

    1.601

    1.437

    222.22

    1.311

    1.298

    1.305

    1.141

    74.04

    0.955

    0.943

    0.949

    0.785

    24.69

    0.726

    0.755

    0.741

    0.577

    8.23

    0.573

    0.584

    0.579

    0.415

    2.74

    0.375

    0.374

    0.375

    0.211

    0

    0.162

    0.165

    0.164

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
  • 基因功能參考文獻：
    1. Downregulation of beta-klotho expression is not the major mechanism of impaired FGF21 signaling in white adipose tissue. PMID: 28580290
    2. liver FGF21-betaKlotho pathway sufficient and necessary to repress mTOR/S6K activity PMID: 26926384
    3. The study supports a pro-adipogenic role for betaKlotho in skeletal muscle fibro/adipogenesis and calls for further research on involvement of the FGF-FGFR-betaKlotho axis in the fibro/adipogenic infiltration associated with functional deterioration of skeletal muscle in aging and muscular dystrophy. PMID: 26881702
    4. Data show that enhancing beta-klotho (KLB) expression in adipose may sensitize to endogenous fibroblast growth factor 21 (FGF21), thus representing a novel strategy to combat metabolic disease. PMID: 26901091
    5. beta-Kl in hepatocytes is necessary and sufficient for lipid homeostasis, whereas nonhepatic beta-Kl regulates energy metabolism. PMID: 26514166
    6. ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
    7. Klotho is a novel player in the retina, with a clear connection to photoreceptor cell death as well as with an influence on retinal organization. PMID: 23796581
    8. Mice lacking the FGF21 co-receptor, beta-Klotho, in the suprachiasmatic nucleus are refractory to the inhibitory effect of FGF21 on female fertility. PMID: 23933983
    9. The effects of FGF21 are mediated through beta-Klotho expression in the suprachiasmatic nucleus of the hypothalamus and the dorsal vagal complex of the hindbrain. PMID: 23933984
    10. betaKlotho is required for fibroblast growth factor 21 effects on growth and metabolism. PMID: 22958921
    11. demonstrate the first manifest evidence revealing that whereas alpha-Kl and beta-Kl are required for FGF23 and FGF15/hFGF19-mediated signaling pathways in vivo, respectively, beta-Kl appears not to be essential for FGF21-mediated signal transduction in vivo. PMID: 20080590
    12. KLB and FGFR1 form a 1:1 heterocomplex independent of the galectin lattice that transitions to a 1:2 complex upon the addition of FGF21. PMID: 22523080
    13. Differential specificity of endocrine FGF19 and FGF21 to FGFR1 and FGFR4 in complex with KLB. PMID: 22442730
    14. In contrast to wild-type FGF23, FGF23-21c (a variant of FGF23 where the C-terminal domain of FGF23 was replaced with the corresponding regions from FGF21), FGF23-21c gained the ability to activate FGFR1c and FGFR2c in the presence of betaKlotho. PMID: 22370560
    15. function of Klotho family proteins PMID: 22396160
    16. These results indicate that betaKlotho and FGFRs form the cognate FGF-21 receptor complex, mediating FGF-21 cellular specificity and physiological effects. PMID: 18064602
    17. Metabolic regulator betaKlotho interacts with fibroblast growth factor receptor 4 (FGFR4) to induce apoptosis and inhibit tumor cell proliferation. PMID: 20657013
    18. betaKlotho functions as a cofactor essential for fibroblast growth factor 21 activity PMID: 17452648
    19. Data suggested that betaKlotho expression is a crucial determinant of the FGF21 specificity of the target cells upon which it acts in an endocrine fashion. PMID: 18187602
    20. The results of this study indicate that both beta-Klotho and FGF-15/19 repress the apical sodium-dependent bile acid transporter in enterocytes and cholangiocytes. PMID: 18772362

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  • 亞細胞定位：
    Cell membrane; Single-pass type III membrane protein.
  • 蛋白家族：
    Glycosyl hydrolase 1 family, Klotho subfamily
  • 組織特異性：
    Present in liver, muscle and white adipose tissue, but not in kidney (at protein level). Expressed in liver and pancreas, and at lower levels in skin, stomach, skeletal muscle, small intestine and lung.
  • 數據庫鏈接：


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