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  4. Human Trem-like transcript 1 protein(TREML1) ELISA kit

Human Trem-like transcript 1 protein(TREML1) ELISA kit

  • 中文名稱：
    人Trem樣轉錄因子1(TREML1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL024406HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人Trem樣轉錄因子1(TREML1)酶聯免疫試劑盒（CSB-EL024406HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TREML1含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，可用于探索TREML1在免疫調節、神經退行性疾病或心血管疾病中的分子機制，為炎癥相關通路研究、藥物干預效果評估等基礎科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    dJ238O23.3 ELISA Kit; GLTL1825 ELISA Kit; MGC119173 ELISA Kit; PRO3438 ELISA Kit; TLT 1 ELISA Kit; TLT-1 ELISA Kit; TLT1 ELISA Kit; TREM like transcript 1 ELISA Kit; Trem like transcript 1 protein ELISA Kit; Trem-like transcript 1 protein ELISA Kit; TREML 1 ELISA Kit; Treml1 ELISA Kit; Triggering receptor expressed on myeloid cells like 1 ELISA Kit; Triggering receptor expressed on myeloid cells like protein 1 ELISA Kit; Triggering receptor expressed on myeloid cells-like protein 1 ELISA Kit; TRML1_HUMAN ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TREML1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 86-94    
    1:2 Average % 100    
    Range % 95-104    
    1:4 Average % 97    
    Range % 86-102    
    1:8 Average % 102    
    Range % 94-107    
  • 回收率：
    The recovery of human TREML1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 84-97    
    EDTA plasma (n=4) 96 87-100    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.396 2.304 2.350 2.196    
    2000 2.017 1.976 1.997 1.843    
    1000 1.536 1.500 1.518 1.364    
    500 1.006 0.995 1.001 0.847    
    250 0.569 0.551 0.560 0.406    
    125 0.355 0.349 0.352 0.198    
    62.5 0.275 0.266 0.271 0.117    
    0 0.151 0.157 0.154 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Cell surface receptor that may play a role in the innate and adaptive immune response.
  • 基因功能參考文獻：
    1. Study characterized the expression of TREM genes in cerebellum and temporal cortex and determined TREML1 and TREM2 to be the only reliably expressed TREM genes in these brain regions; identified rs9357347 as a putative regulatory variant that is associated with protection from Alzheimer's disease and with increased TREML1 and TREM2 brain levels. PMID: 27939925
    2. Levels of sTLT-1 in plasma from patients with potential acute coronary syndrome were compared with an age-matched control group with similar risk factors for cardiovascular disease. PMID: 25147325
    3. Platelet-derived soluble TLT-1 is a potent endogenous regulator of sepsis-associated inflammation. PMID: 22551551
    4. Serum sTREM-1 level measurements may be helpful in diagnosing and predicting outcome for non-tuberculous mycobacterial lung disease. PMID: 22283904
    5. These results suggest that during inflammation, sTLT-1 may mediate hemostasis by enhancing actin polymerization, resulting in increased platelet aggregation and adherence to the endothelium. PMID: 20093931
    6. initial characterization of TREM-like transcript (TLT)-1 results indicate it is likely that TLT-1 regulates the signaling of the TREM family receptors(TLT-1) PMID: 12393607
    7. In contrast to previously characterized immunoreceptor tyrosine-based inhibition motif (ITIM) receptors, TLT1 enhances rather than inhibits calcium signaling in rat RBL-2H3 cells, a property dependent on the SHP-2 recruiting classical Tyr281 ITIM. PMID: 15128762
    8. A soluble fragment of the TLT-1 extracellular domain is found in serum of humans and mice and that an isoform of similar mass is released from platelets following activation with thrombin. PMID: 16505478
    9. Define a platelet specific function for TLT1 in regulation of aggregation. PMID: 17549298
    10. Phenotypic heterogeneity in the Gray platelet syndrome extends to the expression of TREM family member, TLT-1. PMID: 18612537
    11. antibody against the extracellular domain inhibits platelet aggregation in vitro PMID: 19230638
    12. TLT-1 plays a protective role during inflammation by dampening the inflammatory response and facilitating platelet aggregation at sites of vascular injury PMID: 19436112

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Sequestered in cytoplasmic vesicles in resting platelets (PubMed:15100151). Transported to the cell surface after stimulation by thrombin (PubMed:15100151). Soluble fragments can be released into the serum by proteolysis (PubMed:16505478).
  • 組織特異性：
    Detected in platelets, monocytic leukemia and in T-cell leukemia.
  • 數據庫鏈接：

    HGNC: 20434

    OMIM: 609714

    KEGG: hsa:340205

    STRING: 9606.ENSP00000402855

    UniGene: Hs.117331



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