在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Serotonin N-acetyltransferase(AANAT) ELISA kit

Human Serotonin N-acetyltransferase(AANAT) ELISA kit

  • 中文名稱：
    人芳烷基胺N-乙酰轉移酶(AANAT)酶聯免疫試劑盒
  • 貨號：
    CSB-EL001022HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人芳烷基胺N-乙酰轉移酶(AANAT)酶聯免疫試劑盒（CSB-EL001022HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的AANAT含量。AANAT（褪黑素合成酶）是褪黑素合成過程中的關鍵酶，受光周期調控，參與調節晝夜節律和多種生理過程。研究機制涉及褪黑素合成、晝夜節律調節及睡眠等生理功能的調控。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，科研人員可將其應用于神經生物學、時間生物學或內分泌學領域的機制研究，例如探究不同光照周期下 AANAT 的節律性表達、評估褪黑激素通路相關疾病的動物模型或細胞模型中酶活性變化，以及篩選影響生物鐘調控的小分子化合物。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    AA-NAT ELISA Kit; Aanat ELISA Kit; Aralkylamine N-acetyltransferase ELISA Kit; Arylalkylamine N acetyltransferase ELISA Kit; Serotonin acetylase ELISA Kit; Serotonin N-acetyltransferase ELISA Kit; SNAT ELISA Kit; SNAT_HUMAN ELISA Kit
  • 縮寫：
    AANAT
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AANAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 103-109
    1:2 Average % 96
    Range % 92-98
    1:4 Average % 91
    Range % 86-96
    1:8 Average % 93
    Range % 89-97
  • 回收率：
    The recovery of human AANAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 99-108
    EDTA plasma (n=4) 92 87-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.141 2.302 2.222 2.121
    1000 1.626 1.711 1.669 1.568
    500 1.248 1.358 1.303 1.202
    250 0.791 0.760 0.776 0.675
    125 0.531 0.503 0.517 0.416
    62.5 0.311 0.331 0.321 0.220
    31.25 0.190 0.198 0.194 0.093
    0 0.097 0.105 0.101 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
  • 基因功能參考文獻：
    1. Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction. PMID: 28699174
    2. Data show that arylalkymine N-acetyltransferase (AANAT) levels and melatonin synthesis change after transient receptor potential channel 4 (TRPV4 channel) stimulation in ciliary body epithelial cells. PMID: 28368307
    3. these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences. PMID: 27339900
    4. There is dysregulation of the AANAT/ASMT/melatonin --> melatonin receptor axis in cholangiocarcinoma, which inhibited melatonin secretion and subsequently enhanced CCA growth. PMID: 21778461
    5. The expression of AANAT in epithelial cells of striated ducts in human submandibular glands. PMID: 21437622
    6. The functional expression of human SNA protein was closely associated with the elevated synthesis of N-acetylserotonin and melatonin in transgenic rice plants. PMID: 20586889
    7. Identified 17 sequence changes in AANAT gene of patients with major depression. Show evidence of the association of genetic variability in the AANAT gene with susceptibility to major depression. PMID: 20459461
    8. There is a significant increase in AANAT allele positivity at the single nucleotide polymorphism (alanine 129--> threonine) at between patients with DSPS & controls. AA-NAT could be a susceptibility gene for DSPS. PMID: 12736803
    9. Data suggest that the -263G/C single nucleotide polymorphism of arylalkylamine-N-acetyl-transferase (AA-NAT) may be an important determinant of the late/short sleep pattern. PMID: 15332344
    10. Single nucleotide polymorphisms in the AANAT gene identified thus far cannot explain the observed interindividual differences for nocturnal melatonin profiles in the subjects investigated. PMID: 18569588
    11. AANAT polymorphisms were not associated with adolescent idiopathic scoliosis. PMID: 18794762
    12. Observational study of genotype prevalence and gene-disease association. (HuGE Navigator) PMID: 17503170

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Acetyltransferase family, AANAT subfamily
  • 組織特異性：
    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
  • 數據庫鏈接：

    HGNC: 19

    OMIM: 600950

    KEGG: hsa:15

    STRING: 9606.ENSP00000250615

    UniGene: Hs.431417



主站蜘蛛池模板： 粗大的内捧猛烈进出视频| 国产婷婷精品av在线| a毛片免费全部播放| 欧洲精品无码一区二区三区在线播放| 18禁成人黄网站免费观看久久| 老师粉嫩小泬喷水视频90| 精品熟女日韩中文十区| 亚洲人午夜射精精品日韩| 国产精品国产三级国产an| 四虎永久在线精品免费观看| 日本高清色倩视频在线观看| 亚洲综合av永久无码精品一区二区| 久久久久国产精品人妻aⅴ院| 久久精品成人免费观看| 婷婷综合缴情亚洲| 青青草原亚洲| 朝鲜美女黑毛bbw| 亚洲这里只有久热精品伊人| 男人的天堂免费一区二区视频| 欧美野性肉体狂欢大派对| 女子spa高潮呻吟抽搐| 女人下面毛多水多视频| 无码天堂亚洲国产av麻豆| 日日日日做夜夜夜夜无码| 欧美午夜一区二区福利视频| 国内揄拍国产精品人妻门事件 | 131美女爱做视频国产福利| 欧美老熟妇牲交| 欧美午夜理伦三级在线观看| 亚洲综合久久无码色噜噜赖水| 久久天天躁狠狠躁夜夜爽| 激情五月婷婷| 亚洲乱亚洲乱妇无码| 亚洲人成网线在线播放| 亚洲国产成人一区二区三区| 九九久久精品无码专区| 欧美成人va免费大片视频| 92精品成人国产在线观看| 亚洲精品一线二线三线无人区 | 中文字幕v亚洲日本在线| 日韩欧美国产一区精品|