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Human Ras-related protein Rab-35(RAB35) ELISA kit

  • 中文名稱:
    人Ras相關蛋白Rab-35(RAB35)酶聯免疫試劑盒
  • 貨號:
    CSB-EL019188HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人Ras相關蛋白Rab-35(RAB35)酶聯免疫試劑盒(CSB-EL019188HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RAB35含量。RAB35屬于RAB蛋白家族成員,在細胞內吞、運輸等過程起關鍵作用。其研究機制主要圍繞調節膜泡運輸,如參與細胞有絲分裂時胞質分裂。異常的RAB35功能與腫瘤、神經系統疾病等病理過程相關,為相關疾病治療提供潛在靶點。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,可用于探究RAB35在腫瘤細胞侵襲轉移中的動態變化、神經突觸形成相關分子機制,或評估藥物干預對特定信號通路中RAB35表達水平的影響,為功能研究與靶點驗證提供可靠工具。滿足細胞生物學、分子機制研究及疾病模型構建等科研需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    GTP binding protein RAY ELISA Kit; GTP-binding protein RAY ELISA Kit; H ray ELISA Kit; OTTHUMP00000240256 ELISA Kit; OTTHUMP00000240257 ELISA Kit; OTTHUMP00000240258 ELISA Kit; OTTHUMP00000240259 ELISA Kit; RAB1C ELISA Kit; Rab35 ELISA Kit; RAB35 member RAS oncogene family ELISA Kit; RAB35; member RAS oncogene family ELISA Kit; RAB35_HUMAN ELISA Kit; Ras related protein rab 1c (GTP binding protein ray) ELISA Kit; Ras related protein Rab 1C ELISA Kit; Ras related protein Rab35 ELISA Kit; Ras-related protein Rab-1C ELISA Kit; Ras-related protein Rab-35 ELISA Kit; RAY ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.7 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human RAB35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %89-96
    1:2Average %95
    Range %91-99
    1:4Average %97
    Range %92-102
    1:8Average %90
    Range %85-96
  • 回收率:
    The recovery of human RAB35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-102
    EDTA plasma (n=4)9490-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.852 2.752 2.802 2.630
    15002.200 2.100 2.150 1.978
    7501.371 1.271 1.321 1.149
    3750.790 0.780 0.785 0.613
    187.50.531 0.521 0.526 0.354
    940.413 0.403 0.408 0.236
    470.247 0.237 0.242 0.070
    00.172 0.171 0.172  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
  • 基因功能參考文獻:
    1. A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. PMID: 29662076
    2. The results provide evidence that MICAL1 plays an essential role in the activation of ROS/Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion. PMID: 27430308
    3. The involvement of Rab35 in diverse and apparently unrelated cellular functions can be explained by the central role of this GTPase in regulating phosphoinositides and F-actin, both on endosomes and at the plasma membrane[review]. PMID: 27329675
    4. Data suggest that Rab35, interacting with TBC1D10A, functions in vascular endothelial cells as a negative regulator of histamine-evoked, Ca2+-dependent Weibel-Palade body exocytosis, most likely acting through the downstream effectors ACAP2 and Arf6. (Rab35 = rab GTP-binding protein 53; TBC1D10A = TBC1 domain family member 10A; ACAP2 = centaurin beta2; Arf6 = ADP-ribosylation factor 6) PMID: 28566286
    5. Short-term EGF stimulation if of lung tumor cells can increase the interaction between RUSC2 and GIT2, prolonged stimulation leads to a decrease of their interaction through activating Rab35. PMID: 27238570
    6. Here the authors report that EspG interacts specifically with the small GTPases ARF6 and Rab35 during infection. PMID: 27261256
    7. Authors propose that the precise spatial and temporal activation of Rab35 acts as a major switch for OCRL recruitment on newborn endosomes, post-scission PtdIns(4,5)P2 hydrolysis, and subsequent endosomal trafficking. PMID: 26725203
    8. Rab35 serves as a clathrin-mediated endocytosis detector. Loss of Rab35 input leads to elevated Arf6-GTP and shifts the sorting of clathrin-independent endocytosis cargo proteins to lysosomes. PMID: 25988331
    9. the activation-inactivation cycles of Rab35 and ARF6 are required for the uptake of zymosan and that ACAP2 is an important component that links Rab35/ARF6 signaling during phagocytosis of zymosan. PMID: 26229970
    10. Two somatic RAB35 mutations found in human tumors generate alleles that constitutively activate PI3K/AKT signaling, suppress apoptosis, and transform cells in a PI3K-dependent manner. PMID: 26338797
    11. mutual regulation with arf6 of cell adhesion and migration PMID: 23264734
    12. Rab35 acts as a downstream target of Dvl2 and mediates cell migration. PMID: 23353182
    13. Activated ARF6 reduces Rab35 loading into the endocytic pathway. PMID: 22226746
    14. whereas connecdenn 1 and 2 activate Rab35 for endosomal trafficking, connecdenn 3 uniquely activates Rab35 for its role in actin regulation PMID: 22072793
    15. analysis of DENND1B-S complexed with its substrate Rab35 PMID: 22065758
    16. the phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) 5-phosphatase OCRL, which is mutated in Lowe syndrome patients, is an effector of the Rab35 GTPase in cytokinesis abscission PMID: 21706022
    17. A Small Ras-like GTPase protein Ray was indicated to modulate p53 transcriptional activity of PRPK. PMID: 16600182
    18. localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway; plays an essential role during the terminal steps of cytokinesis PMID: 16950109
    19. EPI64C and Rab35 regulate a recycling pathway in T cells and contribute to immunological synapse formation, most likely by participating in TCR transport to the immunological synapse PMID: 18450757
    20. study found that Rab35 regulates the assembly of actin filaments in filopodia formation; effect was mediated by the actin-bundling protein fascin, which directly associated with active Rab35 PMID: 19729655

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  • 亞細胞定位:
    Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome.
  • 蛋白家族:
    Small GTPase superfamily, Rab family
  • 數據庫鏈接:

    HGNC: 9774

    OMIM: 604199

    KEGG: hsa:11021

    STRING: 9606.ENSP00000229340

    UniGene: Hs.524788



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