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Human Nerve growth factor,NGF ELISA kit

  • 中文名稱:
    人神經生長因子(NGF)酶聯免疫試劑盒
  • 貨號:
    CSB-E04683h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人神經生長因子(NGF)酶聯免疫試劑盒(CSB-E04683h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物.樣本中的NGF含量。神經生長因子(NGF)是一種神經營養因子,對神經系統發育和功能至關重要。其研究機制涉及NGF與受體結合,激活信號通路,調節神經元生長、存活和功能。NGF在神經退行性疾病、疼痛管理等領域具有潛在治療價值。試劑盒檢測范圍為6.86 pg/ml-5000 pg/ml.,靈敏度為6.86 pg/ml.。適用于神經生物學、疾病機制研究及藥物開發等科研領域。通過本試劑盒可定量分析不同實驗條件下(如疾病模型、藥物處理或基因干預)樣本中 NGF 的動態變化,為探究神經系統相關疾病的分子機制、篩選潛在治療靶點或評估藥物對神經保護作用提供可靠工具,適用于實驗室常規分子生物學研究需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Beta nerve growth factor ELISA Kit; Beta NGF ELISA Kit; Beta-nerve growth factor ELISA Kit; Beta-NGF ELISA Kit; HSAN5 ELISA Kit; MGC161426 ELISA Kit; MGC161428 ELISA Kit; Nerve growth factor (beta polypeptide) ELISA Kit; Nerve growth factor ELISA Kit; Nerve growth factor beta ELISA Kit; Nerve growth factor beta polypeptide ELISA Kit; Nerve growth factor beta subunit ELISA Kit; NGF ELISA Kit; NGF_HUMAN ELISA Kit; NGFB ELISA Kit; NID67 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
  • 檢測范圍:
    6.86 pg/ml-5000 pg/ml.
  • 靈敏度:
    6.86 pg/ml.
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    90-100

    1:2

    Average %

    94

    Range %

    90-98

    1:4

    Average %

    92

    Range %

    87-100

    1:8

    Average %

    89

    Range %

    84-95

  • 回收率:

    The recovery of human NGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    85-97

    EDTA plasma (n=4)

    92

    88-99

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.813

    2.864

    2.839

    2.687

    1666.7

    2.346

    2.351

    2.349

    2.197

    555.6

    1.722

    1.752

    1.737

    1.585

    185.2

    1.133

    1.148

    1.141

    0.989

    61.7

    0.670

    0.683

    0.677

    0.525

    20.6

    0.483

    0.491

    0.487

    0.335

    6.86

    0.274

    0.283

    0.279

    0.127

    0

    0.150

    0.153

    0.152

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form.
  • 基因功能參考文獻:
    1. the effects of secretome-enriched conditioned medium (CM) of neuronally differentiated PC12 cells on the neuronal differentiation of human mesenchymal stem cells via NGF like mechanism, is reported. PMID: 29527653
    2. Single nucleotide polymorphisms in NGF gene (rs6330) and NGFR gene (rs2072446 and rs734194) are associated with ischemic stroke. The NGF rs6330*T and NGFR rs2072446*T minor alleles might be nominated as a risk factor for developing ischemic stroke and NGFR rs734194*G minor allele as a protective against this disease at least in Armenian population PMID: 29499660
    3. Neurotrophic factors and hippocampal activity in PTSD PMID: 29799860
    4. SNRPA may contribute to GC progression via NGF and could be a prognostic biomarker for GC. PMID: 30039889
    5. Results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth. PMID: 28465581
    6. The results suggest an interaction between NGF, GDNF and MMP-9 during the transition to malignancy in prostate cancer (PC). Also this interaction may involve in regulating PC cell differentiation, tumor invasion, progression, and the agressiveness of PC. PMID: 28237042
    7. the anti-tumor activity of oleuropein against hepatocellular carcinoma could be attributed to influencing the pro-NGF/NGF balance via affecting MMP-7 activity without affecting the gene expression of NGF PMID: 29476769
    8. co-expression patterns of NGF and heme oxygenase-1 might be used as prognostic indicators for gastric carcinoma patients PMID: 28679437
    9. This review will briefly address the peripheral and central sensitization mechanisms of airway neurons and will then focus on NGF signaling and its role in cough hypersensitivity.[review] PMID: 28494216
    10. First-trimester plasma nerve growth factor is lower in patients who subsequently develop preeclampsia. PMID: 27513943
    11. This study show evidence of variation in plasmatic beta-NGF expression during the progression of dementia. PMID: 27802234
    12. NGF is functionally linked to beta-catenin, promoting the migration of human ovarian cancer cells via the WNT/beta-catenin pathway. PMID: 27835587
    13. Varicella zoster virus DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent. PMID: 27683235
    14. Many studies indicate that the only presence of NGF is unable to generate cell carcinogenesis, both in normal neuronal and non-neuronal cells/tissues. However, it cannot be excluded the possibility that the co-expression of NGF and pro-carcinogenic molecules might open to different consequence. [review] PMID: 27439311
    15. the effect and underlying mechanisms of NGF/BDNF on the production of NPW in PC12 cells and hypothalamus, is reported. PMID: 28249734
    16. These data support a role for islet NGF in fine-tuning insulin secretion. PMID: 27424144
    17. The results of this study indicated that dysmenorrhea pain severity is partly genetically determined by the chromosome 1p13.2, near the nerve growth factor locu. PMID: 27454463
    18. Findings suggest that IL-1beta and TNF-alpha regulate Nerve Growth Factor expression and production in synovial macrophages and fibroblasts in osteoarthritic joints. PMID: 28677145
    19. These results advance our knowledge of the conformational properties of proNGF and NGF and help provide a rationale for the diverse biological effects of NGF and proNGF at the molecular level. PMID: 28798232
    20. The results of this study suggest that proNGF protein levels may augment the diagnostic accuracy of currently used CSF biomarker panels. PMID: 26825093
    21. Neuroimmune-endocrine events may lead to overactivity of sympathetic nervous system that triggers cascade of pathologic conditions in ovary in polycystic ovary syndrome (PCOS). Data suggest women with PCOS exhibit reduction of CRH and NGF; reduction of CRH/NGF may be under influence of sympathetic nervous system and may reflect deficit of neuronal stress-adaptation in PCOS patients. (CRH = corticotropin releasing hormone) PMID: 27908212
    22. The rare nerve growth factor-beta (NGFB) mutation R221W causes a selective loss of thinly myelinated fibers and especially unmyelinated C-fibers. Carriers of this mutation show altered pain sensation. PMID: 27146986
    23. NGF expression was positively correlated with disease severity and visceral hypersensitivity in irritable bowel syndrome patients. PMID: 27862119
    24. a stage-related modulation of beta-NGF and its receptors in the inflammatory process of OA PMID: 28253191
    25. BDNF and NGF serum levels are reduced in the early and moderate glaucoma stages, suggesting the possibility that both factors could be further investigated as potential circulating biomarkers for the early detection of glaucoma. PMID: 28068360
    26. overexpression of ARMS blocked NGF-mediated secretion, without affecting basal secretion, a decrease in ARMS resulted in potentiation. Similar effects were observed with synembryn-B, a protein that interacts directly with ARMS. Downstream of ARMS and synembryn-B are Galphaq and Trio proteins, which modulate the activity of Rac1 in response to NGF PMID: 26966186
    27. Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
    28. Results indicate that NGF inhibited CRT translocation induced by mitoxantrone. NGF effect on CRT translocation could have consequences in immunotherapy, potentially lessening the effectiveness of this type of treatment. PMID: 28260038
    29. NGF stimulates generation of neurons, but not neuronal progenitors from embryonic stem cells and affects the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. PMID: 28364186
    30. NGF attenuates these responses-both in vivo and in vitro. Therefore, NGF therapy may represent a novel approach for the management of diabetic keratopathy. PMID: 27978558
    31. NGF signaling pathway provides a potential target for developing molecularly targeted therapies PMID: 27792755
    32. these data suggest a positive feedback loop through which NGF-mediated upregulation of p75(NTR) can contribute to the chemo-resistance of Triple negative breast cancer cells. PMID: 27577679
    33. Based on these current developments, the present review provides not only a broad overview of the biological effects of NGF-TrkA-p75NTR on cancer cells and their microenvironment, but also explains why NGF and its receptors are going to evoke major interest as promising therapeutic anti-cancer targets in the coming decade. PMID: 27264679
    34. NGF/CD133 might be an effective and potent therapeutic target for pancreatic cancer metastasis, particularly in perineural invasion PMID: 27654574
    35. The analysis of covariance (ANCOVA) indicated that the mean serum GDNF and NTF3 levels of ADHD patients were significantly higher than that of controls. However, serum BDNF and NGF levels did not show any significant differences between groups. PMID: 27561780
    36. All patients had serum neurotrophin (NT-3, BDNF, NGF) concentrations determined. PMID: 27367919
    37. Serum NGF does not differentiate between recurrent acute pancreatitis and chronic pancreatitis. PMID: 27020638
    38. NGF FLIPs TrkA onto the death TRAIL in neuroblastoma cells PMID: 26962689
    39. Major depression patients had similar serum NGF to controls. PMID: 27008247
    40. data demonstrate the involvement and modulation of nerve growth factor and its receptors in chronic obstructive pulmonary disease and in its staging PMID: 26408608
    41. The present study showed that IL-17, in addition to stimulating an inflammatory response, negatively regulates the action of NGF and NGF R in the polar forms of the leprosy. PMID: 26616164
    42. Locally increased estrogen levels and inflammation may cause increased NGF production in the uterus of patients with adenomyosis. PMID: 25519715
    43. The present study is the first to thoroughly assess the enhancement of neural differentiation of bone marrow mesenchymal stem cells following transfection with bFGF and NGF. PMID: 26572749
    44. Data suggest MMP7 (matrix metalloproteinase 7) in follicular fluid cleaves proNGF (pro-nerve growth factor) in ovarian follicle; both MMP7 and proNGF appear to be products of granulosa cells; processing of proNGF to NGF appears to regulate apoptosis. PMID: 26457789
    45. NGF promotes renal fibrosis via TGF-beta1-signaling activation, suggesting that in kidney diseases high NGF serum levels could contribute to worsen renal fibrosis. PMID: 26066770
    46. This study suggests that the circadian rhythm in the esophagus may be important for the mediation of and/or the response to erosive damage in GERD patients. PMID: 26337663
    47. Intratumoral nerve growth factor expression is not associated with perineural invasion in patients with resected extrahepatic cholangiocarcinoma. PMID: 26547754
    48. NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid PMID: 26569118
    49. NGF-induced tyrosine kinase independent TrkA signaling through CD44 is sufficient to maintain tumor aggressiveness in breast cancer PMID: 25840418
    50. Urinary NGF, but not BDNF, levels decreased significantly after hyaluronic acid therapy. PMID: 24614892

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  • 相關疾病:
    Neuropathy, hereditary sensory and autonomic, 5 (HSAN5)
  • 亞細胞定位:
    Secreted. Endosome lumen.
  • 蛋白家族:
    NGF-beta family
  • 數據庫鏈接:

    HGNC: 7808

    OMIM: 162030

    KEGG: hsa:4803

    STRING: 9606.ENSP00000358525

    UniGene: Hs.2561



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