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中文名稱:人再生蛋白(NEO1)酶聯免疫試劑盒
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貨號:CSB-EL015712HU
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人再生蛋白(NEO1)酶聯免疫試劑盒(CSB-EL015712HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NEO1含量。NEO1 即神經纖毛蛋白 - 1,它屬于細胞表面受體,在胚胎發育、血管生成等生理過程中發揮重要作用。其研究機制主要聚焦在與配體相互作用,調節細胞信號通路,進而影響細胞的增殖、遷移和存活,目前在腫瘤等疾病的治療研究中受關注。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,該產品適用于體外研究NEO1在細胞遷移、組織修復模型中的調控機制,或用于評估藥物干預、基因編輯等實驗條件下目標蛋白的表達特征,為再生醫學基礎研究、疾病模型構建及生物標志物探索提供精準的定量分析工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:Immunoglobulin superfamily DCC subclass member 2 ELISA Kit; NEO1 ELISA Kit; NEO1_HUMAN ELISA Kit; Neogenin ELISA Kit; Ngn (gene name) ELISA Kit
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縮寫:NEO1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:78 pg/mL-5000 pg/mL
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靈敏度:19.5 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human NEO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 94-104 1:2 Average % 89 Range % 83-97 1:4 Average % 92 Range % 87-97 1:8 Average % 111 Range % 106-114 -
回收率:
The recovery of human NEO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-99 EDTA plasma (n=4) 105 103-111 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. v pg/ml OD1 OD2 Average Corrected 5000 2.084 2.020 2.052 1.933 2500 1.403 1.454 1.429 1.310 1250 1.051 1.098 1.075 0.956 625 0.712 0.721 0.717 0.598 312 0.358 0.365 0.362 0.243 156 0.245 0.254 0.250 0.131 78 0.177 0.178 0.178 0.059 0 0.115 0.122 0.119 -
數據處理:
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貨期:3-5 working days
引用文獻
- Netrin-1 Promotes Visceral Adipose Tissue Inflammation in Obesity and Is Associated with Insulin Resistance A Mentxaka,Nutrients,2022
- Inhibition of neogenin fosters resolution of inflammation and tissue regeneration Martin Schlegel.et al,journal of clinical investigation,2018
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■ 常見問題解答
靶點詳情
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功能:Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.
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基因功能參考文獻:
- Overexpression of Neo1 significantly reduced the GC cell sensitivity to cisplatin and increased the cell viability, motility and adhesion ability, and while silencing of Neo1 showed contrary results. PMID: 30064133
- Endogenous NTN4/NEO1 maintain neuroblastoma growth via both pro-survival and pro-migratory molecular signaling. PMID: 28038459
- Environmental and endogenous proteases may contribute to cancer development by depleting DCC and neogenin. PMID: 27716118
- these results suggested that GD3 recruits gamma-secretase to lipid/rafts, allowing efficient cleavage of neogenin. PMID: 27288875
- The hemizygous 15q deletion unmasks the recessive functional polymorphism in NEO1 which plays a pivotal role in cortical interneuron development; study provides the first evidence linking NEO1 with autism spectrum disorders in humans PMID: 26518331
- Neogenin influences both cadherin dynamics and junctional tension. PMID: 27029596
- Ntn4 promotes the proliferation and motility of gastric cancer cells which is mediated by its receptor Neo and through further activation of multi-oncogenic pathways PMID: 25909166
- The present study demonstrates that neogenin may be a tumor suppressor in breast cancer. Neogenin may serve as a potential diagnostic marker and therapeutic target for breast cancer. PMID: 25998984
- These results show how repulsive guidance molecule acts as the central hub that links BMP2 and NEO1 and physically connects these fundamental signaling pathways. PMID: 25938661
- Neogenin expression is inversely associated with breast cancer grade. PMID: 25416629
- high-expression of neogenin-1 promotes gastric cancer proliferation and motility PMID: 24930499
- miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin. PMID: 24657544
- these results highlight Neogenin1 as a novel downstream effector of the Sonic Hedgehog pathway in medulloblastoma PMID: 23775842
- two RGMB ectodomains conformationally stabilize the juxtamembrane regions of two NEO1 receptors in a pH-dependent manner. PMID: 23744777
- neogenin forms a ternary complex with both MT2 and HJV at the plasma membrane. The complex facilitates HJV cleavage by MT2, and release of the cleaved HJV from the cell occurs after a retrograde trafficking through the TGN/Golgi compartments. PMID: 22893705
- pattern of distribution suggests that a functional netrin-4-neogenin pathway might be restricted to syncytiotrophoblasts, mesenchymal cells, and villous endothelial cells PMID: 22705235
- the proposed role of neogenin as a tumor suppressor in gliomas PMID: 22666451
- identification of neogenin-binding site on the repulsive guidance molecule A PMID: 22396795
- Netrin-1 can promote the potential of proliferation and invasion of extravillous trophoblasts in vitro through its receptors neogenin and UNC5B. PMID: 19570425
- Neogenin FN5, which does not bind hemojuvelin in isolation, exhibits a highly electropositive surface, which may be involved in interactions with negatively-charged polysaccharides or phospholipids in the membrane bilayer. PMID: 20971194
- neogenin negatively regulates the functions of BMP and that this effect of neogenin is mediated by the activation of RhoA. PMID: 21149453
- results show that different isoforms of hemojuvelin may play unique physiological roles through defined interactions with distinct signaling proteins such as BMP2 and neogenin, and demonstrate that some of these interactions are defective. PMID: 18287331
- This study supports the hypothesis that, as in the embryo, neogenin regulates fundamental signalling pathways important for neurogenesis in the adult mouse and human forebrain. PMID: 20575069
- neogenin and restin have roles in proproliferation/survival action on ovarian cancer cells PMID: 12833147
- neogenin may play a role in mammary carcinogenesis as well as morphogenesis PMID: 16324219
- HJV-induced BMP signaling and hepcidin expression are not altered by neogenin overexpression or by inhibition of endogenous neogenin expr PMID: 18326817
- the neogenin intracellular domain regulates gene transcription via nuclear translocation PMID: 18391016
- neogenin-mediated HJV release occurs after the HJV-neogenin complex is internalized from the cell surface PMID: 18445598
- Results show that the serine/threonine kinase death-associated protein kinase (DAPK) is involved in the signal transduction of neogenin. PMID: 18583991
- Results support that hepatic neogenin possesses two functions, mediation of cellular HJV release, and stimulation of HJV-enhanced hepcidin expression. PMID: 19564337
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亞細胞定位:Cell membrane; Single-pass type I membrane protein.
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蛋白家族:Immunoglobulin superfamily, DCC family
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組織特異性:Widely expressed and also in cancer cell lines.
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數據庫鏈接:
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