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Human Melatonin,MT ELISA Kit

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產品描述：

人褪黑素(MT)酶聯免疫試劑盒（CSB-E08132h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MT含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，兼容多種生物樣本，可應用于神經生物學、睡眠研究、內分泌調控機制等科研領域，例如探究晝夜節律紊亂模型中的激素水平變化，或評估藥物干預對褪黑素分泌的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

N/A

縮寫：

MT

種屬：

Homo sapiens (Human)

樣本類型：

serum, plasma, tissue homogenates

檢測范圍：

6.25 pg/mL-400 pg/mL

靈敏度：

1.56 pg/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Epigenetics and Nuclear Signaling

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	99
	Range %	92-106
1:4	Average %	88
	Range %	82-93
1:8	Average %	90
	Range %	84-94

回收率：

The recovery of human MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	103	95-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.451	2.357	2.404	2.323
200	1.627	1.616	1.622	1.541
100	1.046	1.003	1.025	0.944
50	0.437	0.473	0.455	0.374
25	0.274	0.285	0.280	0.199
12.5	0.189	0.196	0.193	0.112
6.25	0.143	0.141	0.142	0.061
0	0.082	0.079	0.081

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled MT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.