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  4. Human Metallothionein,MT ELISA Kit

Human Metallothionein,MT ELISA Kit

  • 中文名稱：
    人金屬硫蛋白(MT)酶聯免疫試劑盒
  • 貨號：
    CSB-E09060h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人金屬硫蛋白(MT)酶聯免疫試劑盒（CSB-E09060h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、尿液、唾液樣本中的MT含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，靈敏度為0.813 pg/mL。適用于科研領域中對MT蛋白表達動態的探究，例如重金屬暴露模型的毒性機制研究、氧化應激相關信號通路解析、疾病動物模型或細胞實驗中MT的調控規律驗證等。試劑盒兼容多種樣本類型，為跨體系、多維度實驗設計提供靈活支持，助力基礎研究中對MT生理功能及分子機制的深入探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CES 1 ELISA Kit; CES1 ELISA Kit; Metallothionein 1A ELISA Kit; Metallothionein 1S ELISA Kit; Metallothionein 2 ELISA Kit; Metallothionein 2A ELISA Kit; Metallothionein IA ELISA Kit; Metallothionein II ELISA Kit; Metallothionein-1A ELISA Kit; Metallothionein-IA ELISA Kit; Metallothionein2 ELISA Kit; MGC32848 ELISA Kit; MT 1A ELISA Kit; MT 2 ELISA Kit; MT 2A ELISA Kit; MT IA ELISA Kit; MT II ELISA Kit; MT-1A ELISA Kit; MT-IA ELISA Kit; MT1 ELISA Kit; MT1A ELISA Kit; MT1A_HUMAN ELISA Kit; MT1S ELISA Kit; MT2 ELISA Kit; MT2A ELISA Kit; MTC ELISA Kit
  • 縮寫：
    MT
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, urine, saliva
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    0.813 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 線性度：

     

    To assess the linearity of the assay, samples were spiked with high concentrations of human MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

     

  • 標準曲線：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

     

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MT antibody.
      • Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial HRP-conjugated MT antibody (6 ml/bottle) ---Bind to the MT in the samples or standards and react with the substrate to make the solution chromogenic.
      • Substrate A (1 x 7 ml)
      • Substrate B (1 x 7 ml)
      • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

I want to know the isoforms detected in the CSB-E09060h Human metallothionein ELISA Kit (Colorimetric).

A:
Thanks for your inquiry.
CSB-E09060h
This kit is designed according to: https://www.uniprot.org/uniprot/P04731.

靶點詳情

  • 最新研究進展：
    MT，全名為金屬硫蛋白，是一種含硫的蛋白質，可結合不同的金屬離子。最近的研究表明，MT在多種疾病的發生和發展中發揮著重要作用，包括腫瘤、神經退行性疾病和心血管疾病等。MT還與氧化應激和免疫反應等生物學過程相關。
  • 功能：
    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • 基因功能參考文獻：
    1. Full-length recombinant MT isoform 1a and it's isolated domain fragments were alkylated, changing protein conformation. PMID: 29518586
    2. MT1A is aberrantly silenced by DNA methylation of 5' MT1A CpG island in melanoma. PMID: 28764861
    3. Using a combination of electrospray ionization mass spectrometry (ESI-MS), circular dichroism (CD), and emission spectroscopy, this study reports that the Cu(i) to human apo-MT1A binding mechanism is highly pH-dependent. PMID: 28466911
    4. Disruption of metallothionein I and II genes in mouse produced viable mice with increased susceptibility to cadmium poisoning. PMID: 8290567
    5. Selective cysteine modification of metal-free human metallothionein 1a and its isolated domain fragments: Solution structural properties revealed via ESI-MS. PMID: 28187517
    6. blockade of metallothioneins 1 and 2 constitutes a promising approach for the treatment of conditions which result in muscle atrophy. PMID: 27956698
    7. Neonatal phthalate ester exposure induced placental MTs, FATP1 and HFABP mRNA expression PMID: 26867681
    8. The present study was undertaken to explore further the interrelationship between p53 and metallothioneins. PMID: 27049123
    9. MTF1 heads a hierarchy of zinc sensors, and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular zinc levels (e.g. ZnT1) alters zinc buffering capacity and total cellular zinc content. PMID: 26824222
    10. These results clearly suggest that MT-1 may be involved in AD pathogenesis. PMID: 26836194
    11. Zn(ii) and Cd(ii) metalation of the human MT1a takes place through two distinct pathways. PMID: 26583802
    12. We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
    13. The Zinc and Cadmium exchange kinetics between human MT1A and carbonic anhydrase were examined using time-dependent electrospray ionization mass spectrometry. PMID: 26401817
    14. Modeling of the reactions showed that at both physiological (7.4) and acidic (5.8) pHs, zinc binding and cadmium exchanges occur essentially randomly between two MT1A fragments. PMID: 26167879
    15. Data indicate the calculated equilibrium zinc binding constants of each of the 7 zinc metallothionein 1A species ranged from a high of (log(KF)) 12.5 to a low of 11.8. PMID: 25208334
    16. Low MT1A expression is associateed with lung carcinogenesis. PMID: 23947958
    17. The metal-free, apo-alpha-MT also adopts a folded structure in the presence of the As(3+) even though there is no As(3+) bound. PMID: 24140052
    18. Increased metallothionein expression reflects steroid resistance in renal allograft recipients. PMID: 23763497
    19. Polymorphisms in the MT1A gene may influence excretion of urine uric acid and N-acetyl-beta-D-glucosamine in chronic lead-exposed workers. PMID: 23429061
    20. MT-1A is epigenetically regulated by PU.1 during monocytic differentiation. PMID: 23501100
    21. During the titration with Zn(2+), the electrospray ionization mass spectrometry data show that several metalated species coexist until the fully saturated proteins are formed. PMID: 23506369
    22. MT1A rs11076161 was associated with B-Cd concentrations and Cd-induced kidney toxicity at high exposure levels. PMID: 22995156
    23. A positive correlation between MT and Ki-67 expression was observed for all the studied cases but was even stronger in the metatypic subtype of basal cell carcinoma. PMID: 23042264
    24. Variations in the ability of LAT1/DMT1/MTF1/MT1a to process and transport Hg may not play a significant role in the etiology of autism. PMID: 21798283
    25. Possible role of MT as a marker of cell stress and homeostasis restoration in Graves'disease. PMID: 22090273
    26. The expression of metallothioneins MT1A and MT1X was significantly downregulated during differentiation of Caco-2 cells treated with high levels of zinc. PMID: 21103883
    27. MT-1A, -1F, -1G, -1X and -2A isoforms are significantly down-regulated in proliferating keloid fibroblasts. PMID: 20812968
    28. Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220
    29. Metallothionein, potential interaction partner for ECRG2, might be involved in regulation of cell proliferation and apoptosis and in various physiological processes. PMID: 12970870
    30. metallothionein measured in renal specimens from cadaver kidneys was restricted to tubular cells with no differences between controls and patients with death due to chronic diseases PMID: 15812196
    31. The overexpression of human MT1A gene dynamically affected cell viability, and the effect was influenced by zinc and cadmium ions. PMID: 16087360
    32. MT-II mRNA expression may be involved in cell proliferation in the livers of patients with chronic HCV infection. PMID: 16107899
    33. +647 MT1a genetic polymorphism may be essential for longevity in women. PMID: 16955215
    34. analysis of glial fibrillary acidic protein, metallothionein, and MHC II expression in human, rat and mouse cells PMID: 17008879
    35. The findings define a pathway for cellular metal acquisition. The results suggest a function of MT in intercellular communication. PMID: 17111383
    36. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is metallothionein. PMID: 17373731
    37. The partially metallated and metal-free metallothionein-1a species are stable intermediates and thus may have a potential role in the currently undefined function of metallothionein. PMID: 17388808
    38. The goals of this study were to define the expression of the isoforms of MT 1, 2, 3 at both mRNA and protein levels, in normal prostate, benign prostatic hyperplasia (BPH) and malignant PC-3 cells. PMID: 18208603
    39. the association of the +647 A/C MT1A polymorphism with diabetes mellitus 2 and cardiovascular complications PMID: 18249147
    40. the interleukin-6 and metallothionein 1a genes act in a concerted manner to control zinc-regulated gene expression PMID: 18316168
    41. Metal exchange in metallothioneins: a novel structurally significant Cd(5) species in the alpha domain of MT1A. PMID: 18429853
    42. This study provides the necessary data establishing unambiguously the noncooperative nature of cadmium binding to both isolated domains and the combined beta-domains (beta-rhMT) of human MT-1a. PMID: 18533113
    43. Lupus nephritis is associated with significant alterations in renal MT-I+II expression. Important prognostic information can be deduced from the renal MT-I+II expression profile in systemic lupus erythematosus patients with nephritis. PMID: 18601746
    44. MT-IA mRNA expression in HPBLs may be used as a biomarker for renal dysfunction in occupational cadmium exposure. PMID: 19359654
    45. HIF-1alpha expression qualified as an independent prognostic and characterised an aggressive cancer phenotype associated with an increased expression of MT. PMID: 19529947

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  • 蛋白家族：
    Metallothionein superfamily, Type 1 family
  • 數據庫鏈接：

    HGNC: 7393

    OMIM: 156350

    KEGG: hsa:4489

    STRING: 9606.ENSP00000290705

    UniGene: Hs.655199



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