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Human Lymphatic vessel endothelial hyaluronic acid receptor 1(LYVE1) ELISA kit

  • 中文名稱:
    人淋巴管內皮透明質酸受體1(LYVE1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL013282HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人淋巴管內皮透明質酸受體1(LYVE1)酶聯免疫試劑盒(CSB-EL013282HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的LYVE1含量。LYVE1(淋巴管內皮透明質酸受體-1)是一種在淋巴內皮細胞特異性表達的受體,主要功能是結合透明質酸,參與淋巴管的形成和功能調節。研究顯示,LYVE1在淋巴管生成、免疫細胞遷移和腫瘤淋巴轉移中發揮重要作用。試劑盒檢測范圍為19.5 pg/mL-1250 pg/mL,靈敏度為4.88 pg/mL。適用于基礎科研領域,如探究腫瘤微環境中淋巴管新生機制、評估炎癥性疾病模型中的淋巴系統功能變化,或用于開發基于LYVE1表達水平的生物標志物研究,為相關疾病的分子機制解析提供實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cell surface retention sequence-binding protein 1 ELISA Kit; CRSBP 1 ELISA Kit; CRSBP-1 ELISA Kit; CRSBP1 ELISA Kit; extracellular link domain containing 1 ELISA Kit; extracellular link domain-containing 1 ELISA Kit; Extracellular link domain-containing protein 1 ELISA Kit; HAR ELISA Kit; Hyaluronic acid receptor ELISA Kit; Lymphatic endothelium specific hyaluronan receptor ELISA Kit; lymphatic vessel endothelial hyaluronan receptor 1 ELISA Kit; Lymphatic vessel endothelial hyaluronic acid receptor 1 ELISA Kit; LYVE 1 ELISA Kit; LYVE-1 ELISA Kit; LYVE1 ELISA Kit; LYVE1_HUMAN ELISA Kit; XLKD1 ELISA Kit
  • 縮寫:
    LYVE1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    19.5 pg/mL-1250 pg/mL
  • 靈敏度:
    4.88 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    151.559

    153.518

    157.724

    156.601

    152.398

    154.078

    SD

    0.035

    0.031

    0.032

    0.045

    0.040

    0.039

    CV(%)

    5.185

    4.545

    4.591

    6.494

    5.900

    5.702

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human LYVE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:250

    Average %

    94

    Range %

    90-98

    1:500

    Average %

    85

    Range %

    81-88

    1:1000

    Average %

    97

    Range %

    93-102

    1:2000

    Average %

    86

    Range %

    80-91

  • 回收率:

    The recovery of human LYVE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    107

    104-110

    EDTA plasma (n=4)

    95

    90-100

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1250

    2.826

    2.804

    2.815

    2.717

    625

    1.984

    1.998

    1.991

    1.893

    312.5

    1.214

    1.201

    1.208

    1.110

    156.25

    0.674

    0.698

    0.686

    0.588

    78.125

    0.400

    0.412

    0.406

    0.308

    39

    0.277

    0.282

    0.280

    0.182

    19.5

    0.189

    0.192

    0.191

    0.093

    0

    0.099

    0.096

    0.098

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    LYVE1是一種淋巴管內皮細胞受體,全名為淋巴管內皮細胞酪氨酸酰基化酶受體1。研究表明,LYVE1在淋巴管形成、淋巴液循環、免疫調節等生理過程中發揮重要作用。此外,LYVE1還與腫瘤轉移、炎癥、心血管疾病等多種疾病的發生和發展相關。
  • 功能:
    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 基因功能參考文獻:
    1. LYVE1 was up-regulated in the malignant Middle Cerebral Artery Infarction. PMID: 28828208
    2. this study shows up-regulation of LYVE-1 expression in the fetal circulation of conducting and exchange villi of HIV-infected pre-eclamptics PMID: 27529307
    3. Dendritic cells (DCs) docked to the basolateral surface of lymphatic vessels and transited to the lumen through hyaluronan-mediated interactions with the lymph-specific endothelial receptor LYVE-1. Targeted deletion of the gene Lyve1, antibody blockade or depletion of the DC hyaluronan coat not only delayed lymphatic trafficking of dermal DCs but also blunted their capacity to prime CD8(+) T cell responses in LNs. PMID: 28504698
    4. These findings reveal binding is dependent not just on clustering but also on the biochemical properties of LYVE-1 homodimers. They also mark LYVE-1 as the first Link protein superfamily member requiring covalent homodimerization for function and suggest the interchain disulfide acts as a redox switch in vivo. PMID: 27733683
    5. LYVE1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    6. Immunostaining analyses in psoriasis skin lesions suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis. PMID: 26966180
    7. These results demonstrate the prerequisite of a critical LYVE-1 threshold density and show that hyaluronan binding may be elicited in lymphatic endothelium by surface clustering with divalent LYVE-1 mAbs. PMID: 26823460
    8. We have established a novel, three-protein biomarker panel that is able to detect patients with early-stage pancreatic cancer in urine specimens:LYVE-1, REG1A, and TFF1 were selected as candidate biomarkers PMID: 26240291
    9. Data (including data from studies in knockout mice) suggest LYVE1 mediates adhesion of group A Streptococci (GAS) to lymphatic vesicular endothelium via capsular hyaluronan; this appears to be critical factor for lymphatic trafficking of GAS in vivo. PMID: 26352587
    10. High expression of LYVE-1 is associated with atherosclerotic arteries. PMID: 25318003
    11. High LYVE expression is associated with visceral pleural invasion and lymphatic thromboembolism in non-small-cell lung cancer. PMID: 25141859
    12. Data indicate that detection of lymphatic vascular invasion (LVI) can be optimized by specific D2-40 or LYVE-1 staining. PMID: 21974896
    13. High-low cell surface HA content of tumor cells through the interaction with LYVE-1 leads to adhesion differences. PMID: 23717428
    14. FGF2 binds to LYVE-1 with a higher affinity than any other known LYVE-1-binding molecules, such as hyaluronan or PDGF-BB. Glycosylation of LYVE-1 is important for FGF2 binding. PMID: 23264596
    15. CRSBP-1-associated fibrillar structures are identical to the ER network as evidenced by the co-localization of CRSBP-1 and BiP in these cells PMID: 22673514
    16. LYVE-1 may have value as predictor of outcome in neuroblastoma PMID: 22241180
    17. Significant correlation between LYVE-1 and Prox-1 expression was observed in non-small cell lung cancer. Expression was also correlated with lymph node metastasis. PMID: 21500548
    18. LYVE-1, VEGFR-3 and CD44 all play an important role in lymphangiogenesis, invasion and metastasis PMID: 21912471
    19. these data support interdependent roles for emmprin, LYVE-1 and BCRP in chemotherapeutic resistance for PEL PMID: 21660043
    20. Results suggest that LYVE-1 allows the adhesion of tumor cells through the interaction of HA on the tumor cell membrane with LYVE-1. PMID: 21291635
    21. Intratumoral lymph vessel density marked by LYVE-1 in laryngeal carcinoma was significantly correlated with tumor infiltration and metastasis. PMID: 17144490
    22. Data show that the density of LYVE-1/+ capillaries in skeletal muscle was observed to decrease significantly over the time course of the exercise intervention. PMID: 20863269
    23. findings indicated that LYVE-1 attenuation in sinusoidal endothelium was one of the manifestations of capillarization, and was associated with hepatic disease progression PMID: 19908110
    24. LYVE-1 indicates an increase of lymphangiogenesis in colorectal carcinoma. PMID: 19742347
    25. A study using specific markers of endothelium (CD31) and lymphatic endothelial cells (Lyve-1, Podoplanin) shows that not only angiogenesis but also lymphangiogenesis occurs within the nodule. PMID: 20011036
    26. new approach measuring lymphangiogenesis using LYVE-1, a novel, specific lymphatic marker in breast cancer tissue PMID: 11689016
    27. LYVE-1 is not exclusive to the lymph vessels PMID: 11719431
    28. LYVE-1 does not seem to be a reliable marker for proliferating infantile hemangiomas. PMID: 16424896
    29. findings indicate (1) the importance of LYVE-1, but not CD44, in regulation of HA metabolism in the maternal-fetal interface and fetal circulation, and (2) possible dual blood and lymphatic phenotypic characteristics in fetal endothelium PMID: 16569201
    30. Molecular data based on gene transcriptional profiles of a 3-gene set (GPC3, LYVE1, and survivin) allow a reliable diagnosis of early hepatocellular carcinoma. PMID: 17087938
    31. analysis of inflammation-induced uptake and degradation of the lymphatic endothelial hyaluronan receptor LYVE-1 PMID: 17884820
    32. DC-sign+ CD163+ macrophages expressing hyaluronan receptor LYVE-1 are located within chorion villi of the placenta. PMID: 18078989
    33. Presence of LYVE-1 in pancreatic islets and in some pancreatic endocrine tumors may suggest structure-function relationship of LYVE-1/lymphatic vessel in hormone synthesis and secretion. PMID: 18090227
    34. LYVE-1 immunohistochemistry appeared to be a useful method for detecting lymphatics invaded by cancer cells, and detailed examination of the submucosa around the tumor may be important for predicting LN metastasis. PMID: 18181096
    35. LYVE-1 expression and the lymphatic density of the muscularis propria are significantly greater than those of other layers of urothelium, including the epithelium, lamina propria, perivesical fat, and serosa PMID: 18342921
    36. LYVE-1 is expressed in a reticulum cell neoplasm in an axillary lymph node. This reticulum cell sarcoma is a lymphatic sinus lining cell sarcoma which might represent another subtype of reticulum cell sarcomas. PMID: 18386053
    37. The normal adult human choroid does not contain typical lymph vessels, but is endowed with a significant number of LYVE-1 positive macrophages. PMID: 18689706
    38. complexity in the regulation of LYVE-1 function and raise the possibility that this receptor, like CD44, may become active after appropriate unmasking in vivo. PMID: 19033446
    39. LYVE-1 immunostaining in some gastrointestinal carcinoids may support a structure-function relationship of lymphatic vessels/hyaluronan receptor for modulating synthesis and secretion of hormones and amines by carcinoid tumour cells. PMID: 19291537
    40. CRSBP-1 exhibits dual ligand (CRS-containing growth regulators (v-sis gene product and insulin-like growth factor binding protein-3, IGFBP-3) and hyaluronic acid) binding activity and plays a role in autocrine regulation of cell growth. PMID: 12912978

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  • 亞細胞定位:
    Membrane; Single-pass type I membrane protein. Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • 組織特異性:
    Mainly expressed in endothelial cells lining lymphatic vessels.
  • 數據庫鏈接:

    HGNC: 14687

    OMIM: 605702

    KEGG: hsa:10894

    STRING: 9606.ENSP00000256178

    UniGene: Hs.655332



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