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  4. Human Lipopolysaccharides,LPS ELISA Kit

Human Lipopolysaccharides,LPS ELISA Kit

  • 中文名稱：
    人脂多糖/內毒素(LPS)酶聯免疫試劑盒
  • 貨號：
    CSB-E09945h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人脂多糖/內毒素(LPS)酶聯免疫試劑盒（CSB-E09945h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖，是革蘭氏陰性菌細胞壁成分。它可激活機體免疫反應，啟動炎癥信號通路，在免疫調節、疾病發生發展中扮演重要角色。研究其作用機制有助于理解炎癥性疾病發病原理，為開發針對性治療方案提供依據。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于研究感染性疾病模型中內毒素釋放規律、炎癥信號通路激活機制，或用于藥物開發過程中化合物對LPS介導炎癥反應的調控作用評估，為免疫學、微生物學及藥理學等領域的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    LPS
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-94  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 94  
    Range % 89-99  
  • 回收率：
    The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-97  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.809 2.960 2.885 2.720  
    200 2.227 2.366 2.297 2.132  
    100 1.515 1.556 1.536 1.371  
    50 0.789 0.755 0.772 0.607  
    25 0.432 0.470 0.451 0.286  
    12.5 0.303 0.325 0.314 0.149  
    6.25 0.235 0.232 0.234 0.069  
    0 0.164 0.165 0.165 　  
  • 本試劑盒所含材料：
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

為什么不同的物種有不同的ELISA試劑盒。是因為LPS的變化嗎?我可以用人LPS ELISA檢測小鼠血漿中的LPS嗎?

A:
不建議跨物種檢測。因為不同物種基質是不同的。

靶點詳情

  • 最新研究進展：
    LPS是一種脂多糖，存在于細菌細胞壁中，可引發嚴重的炎癥反應。最近的研究表明，LPS可以激活多種免疫細胞，如單核細胞和樹突狀細胞，并誘導它們釋放多種炎癥介質。此外，LPS也被認為是引發多種疾病的重要因素之一，例如感染、腸道炎癥和自身免疫性疾病等。研究人員正在努力開發抑制LPS介導的免疫反應的新型治療藥物。


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