Bovine lipopolysaccharides (LPS) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛脂多糖/內(nèi)毒素(LPS)酶聯(lián)免疫試劑盒（CSB-EQ027975BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖，是革蘭氏陰性菌細(xì)胞壁的主要成分。其具有免疫原性，可激活免疫系統(tǒng)。研究機(jī)制主要圍繞其與免疫細(xì)胞表面受體結(jié)合，觸發(fā)信號傳導(dǎo)通路，導(dǎo)致炎癥介質(zhì)釋放等，在感染性疾病、炎癥相關(guān)研究中有重要意義。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于動物模型（如牛、小鼠等）中LPS水平的動態(tài)監(jiān)測，例如評估細(xì)菌感染后內(nèi)毒素釋放量、炎癥反應(yīng)程度或藥物干預(yù)效果等研究；可為體外實(shí)驗或動物實(shí)驗提供可靠的數(shù)據(jù)支持，助力科研人員在免疫學(xué)、微生物學(xué)及藥理學(xué)等領(lǐng)域探索LPS相關(guān)分子機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LPS
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-104
1:2	Average %	102
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	90-99

回收率：

The recovery of bovine LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	89	84-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.