在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Ketohexokinase(KHK) ELISA kit

Human Ketohexokinase(KHK) ELISA kit

  • 中文名稱：
    人己酮糖激酶(KHK)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012157HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人己酮糖激酶(KHK)酶聯免疫試劑盒（CSB-EL012157HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的KHK含量。KHK即己酮糖激酶，是催化果糖代謝第一步反應的關鍵酶。研究顯示其與代謝綜合征等多種疾病密切相關。其研究機制主要聚焦于KHK對果糖代謝通路的調控，以及通過影響肝臟脂質合成等途徑，在疾病發(fā)生發(fā)展中所起的作用。試劑盒檢測范圍為18.75 mU/mL-1200 mU/mL，適用于細胞能量代謝研究、動物模型代謝評估及體外藥物干預實驗等科研領域，可為果糖代謝相關分子機制探索、代謝綜合征病理研究及潛在治療靶點篩選提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
  • 縮寫：
    KHK
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 mU/mL-1200 mU/mL
  • 靈敏度：
    4.68 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-94
    1:2Average %84
    Range %80-88
    1:4Average %95
    Range %90-99
    1:8Average %99
    Range %93-103
  • 回收率：
    The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-102
    EDTA plasma (n=4)9086-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    12002.547 2.461 2.504 2.352
    6002.143 2.076 2.110 1.958
    3001.403 1.449 1.426 1.274
    1500.814 0.765 0.790 0.638
    750.304 0.325 0.315 0.163
    37.50.226 0.218 0.222 0.070
    18.750.182 0.169 0.176 0.024
    00.156 0.147 0.152 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
  • 基因功能參考文獻：
    1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
    2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
    3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
    4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
    5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
    6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
    7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
    8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
    9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
    10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
    11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742

    顯示更多

    收起更多

  • 相關疾病：
    Fructosuria (FRUCT)
  • 蛋白家族：
    Carbohydrate kinase PfkB family
  • 組織特異性：
    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • 數據庫鏈接：

    HGNC: 6315

    OMIM: 229800

    KEGG: hsa:3795

    STRING: 9606.ENSP00000260598

    UniGene: Hs.567297



主站蜘蛛池模板： 丰满多毛的大隂户毛茸茸| 亚洲一区av无码专区在线观看| 无码av一区二区三区无码| 国模少妇一区二区三区| 又粗又猛又黄又爽无遮挡| 国语对白刺激精品视频| 久久毛片免费看一区二区三区| 日韩中文字幕在线专区| 无码国内精品久久综合88| 韩国精品一区二区无码视频| 日本一区二区更新不卡| 人人做人人爽国产视| 午夜免费啪在线观看视频| 人妻av中文字幕无码专区| 欧美老熟妇乱大交xxxxx| 污18禁污色黄网站免费| 免费视频好湿好紧好大好爽| 精品无人码麻豆乱码1区2区 | 99在线在线视频观看| 丁香色欲久久久久久综合网| 亚洲日本va午夜中文字幕久久| 蜜臀少妇人妻在线| 99久久久精品免费观看国产| 成人性生交大片免费看96| 成在人线av无码免费高潮喷水| 无码少妇一区二区浪潮av| 国产丶欧美丶日本不卡视频| 少妇内射兰兰久久| 国产成人片无码免费视频软件 | 国产精品久久一区二区三区| 中文人妻无码一区二区三区信息| 天堂网www在线资源最新版 | 国内精品乱码卡一卡2卡| 国产人妻一区二区三区四区五区六| 福利姬液液酱喷水| 亚洲性无码一区二区三区| 中文字幕韩国三级理论| 顶级欧美熟妇xx| 国产成人无码av在线影院| 香草乱码一二三四区别| 精品久久久久久|