在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Ketohexokinase(KHK) ELISA kit

Human Ketohexokinase(KHK) ELISA kit

  • 中文名稱：
    人己酮糖激酶(KHK)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012157HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人己酮糖激酶(KHK)酶聯免疫試劑盒（CSB-EL012157HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的KHK含量。KHK即己酮糖激酶，是催化果糖代謝第一步反應的關鍵酶。研究顯示其與代謝綜合征等多種疾病密切相關。其研究機制主要聚焦于KHK對果糖代謝通路的調控，以及通過影響肝臟脂質合成等途徑，在疾病發(fā)生發(fā)展中所起的作用。試劑盒檢測范圍為18.75 mU/mL-1200 mU/mL，適用于細胞能量代謝研究、動物模型代謝評估及體外藥物干預實驗等科研領域，可為果糖代謝相關分子機制探索、代謝綜合征病理研究及潛在治療靶點篩選提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
  • 縮寫：
    KHK
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 mU/mL-1200 mU/mL
  • 靈敏度：
    4.68 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-94
    1:2Average %84
    Range %80-88
    1:4Average %95
    Range %90-99
    1:8Average %99
    Range %93-103
  • 回收率：
    The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-102
    EDTA plasma (n=4)9086-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    12002.547 2.461 2.504 2.352
    6002.143 2.076 2.110 1.958
    3001.403 1.449 1.426 1.274
    1500.814 0.765 0.790 0.638
    750.304 0.325 0.315 0.163
    37.50.226 0.218 0.222 0.070
    18.750.182 0.169 0.176 0.024
    00.156 0.147 0.152 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
  • 基因功能參考文獻：
    1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
    2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
    3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
    4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
    5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
    6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
    7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
    8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
    9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
    10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
    11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742

    顯示更多

    收起更多

  • 相關疾病：
    Fructosuria (FRUCT)
  • 蛋白家族：
    Carbohydrate kinase PfkB family
  • 組織特異性：
    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • 數據庫鏈接：

    HGNC: 6315

    OMIM: 229800

    KEGG: hsa:3795

    STRING: 9606.ENSP00000260598

    UniGene: Hs.567297



主站蜘蛛池模板： 亚洲中文字幕无码久久| 国产白嫩漂亮美女在线观看| 午夜理论片在线观看免费| 最新 国产 精品 精品 视频| 性开放的欧美大片| 欧美拍拍视频免费大全| 中文字幕乱妇无码av在线| yy6080久久伦理一区二区| 欧美日产国产新一区| 亚洲国产香蕉碰碰人人| 国产精品久久久久久av| 热re99久久精品国产66热| 国产午夜aaaaa片在线影院| 日韩视频无码免费一区=区三区| 中文字幕精品一二三四五六七八| 国产午夜精品一区理论片飘花| 欧美牲交a欧美牲交aⅴ免费下载| 亚洲 综合 校园 欧美 制服| 五十路熟妇强烈无码| 国产精品一区二区av不卡| 中文字幕无码中文字幕有码| 99国产欧美另类久久久精品| 国产98在线 | 免费| 日日碰狠狠丁香久燥| 久久久中文久久久无码| 丁香五月开心婷婷激情综合| a毛片终身免费观看网站| 国产亚洲精品一区二区三区| 国产97超碰人人做人人爱| 亚洲高清乱码午夜电影网制服| 午夜丰满少妇性开放视频| 久久99精品久久久久婷婷| 久久一本久综合久久爱| 亚洲va中文字幕无码一二三区| 青青草视频在线观看| 欧美激情精品久久久久久| 99精品产国品一二三产区| 99久久人妻精品免费一区| 国产成a人片在线观看视频下载| 国产精品国产亚洲区艳妇糸列短篇 | 欧美性受xxxx黑人xyx性爽|