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  4. Human Insulin-like growth factor-binding protein 6(IGFBP6) ELISA kit

Human Insulin-like growth factor-binding protein 6(IGFBP6) ELISA kit

  • 中文名稱：
    人胰島素樣生長因子結合蛋白6(IGFBP6)酶聯免疫試劑盒
  • 貨號：
    CSB-EL011100HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人胰島素樣生長因子結合蛋白6(IGFBP6)酶聯免疫試劑盒（CSB-EL011100HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的IGFBP6含量。IGFBP6是一種重要靶點。它能與胰島素樣生長因子結合，尤其對IGF-II有高親和力。其背景方面涉及細胞生長、增殖等生理過程。研究機制主要圍繞其對IGF-II活性的抑制，以此影響腫瘤細胞的生長、遷移等，在腫瘤等疾病研究中有潛在價值。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，靈敏度為0.195 ng/mL。該產品適用于體外研究IGFBP6在腫瘤微環境調控、代謝紊亂機制、細胞增殖與凋亡等相關領域的表達變化，為探索IGF信號通路在疾病發生發展中的作用提供可靠工具，尤其適用于需要高靈敏度檢測微量樣本的科研場景，如臨床前模型的組織分析或體液生物標志物研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IBP 6 ELISA Kit; IBP-6 ELISA Kit; IBP6 ELISA Kit; IBP6_HUMAN ELISA Kit; IGF binding protein 6 ELISA Kit; IGF-binding protein 6 ELISA Kit; IGFBP 6 ELISA Kit; IGFBP-6 ELISA Kit; IGFBP6 ELISA Kit; Insulin like growth factor binding protein 6 ELISA Kit; Insulin-like growth factor-binding protein 6 ELISA Kit
  • 縮寫：
    IGFBP6
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma,tissue homogenates
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    6.168

    6.334

    6.306

    6.272

    6.359

    6.242

    SD

    0.035

    0.038

    0.042

    0.048

    0.054

    0.051

    CV(%)

    3.403

    3.625

    4.019

    4.611

    5.136

    4.916

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:5

    Average %

    96

    Range %

    92-103

    1:10

    Average %

    90

    Range %

    86-98

    1:20

    Average %

    93

    Range %

    89-99

    1:40

    Average %

    91

    Range %

    85-98

  • 回收率：

    The recovery of human IGFBP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    95

    89-102

    EDTA plasma (n=4)

    92

    88-99

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    50

    3.109

    3.089

    3.099

    2.949

    25

    2.418

    2.425

    2.421

    2.271

    12.5

    1.645

    1.665

    1.655

    1.505

    6.25

    1.056

    1.034

    1.045

    0.895

    3.13

    0.666

    0.635

    0.650

    0.500

    1.56

    0.379

    0.363

    0.371

    0.221

    0.78

    0.277

    0.262

    0.269

    0.119

    0

    0.154

    0.147

    0.150

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
    IGFBP6的最新研究表明，它可能參與了神經系統的發育和功能。一項研究發現，IGFBP6可以促進神經元的生成和神經元的細胞周期進程。另外，IGFBP6也被發現可以通過影響細胞的能量代謝和凋亡過程來調節癌細胞的增殖和生存。
  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration.
  • 基因功能參考文獻：
    1. A decrease of IGFBP6 expression can involve a decrease in the expression of N-cadherin and transcription factor Slug. PMID: 29577195
    2. IGFBP-6 could inhibit invasion and migration of colorectal carcinoma cells PMID: 29439316
    3. IGFBP6 might be an important regulator and prognostic factor for glioma. PMID: 27650075
    4. Results suggest that insulin like growth factor binding protein 6 (IGFBP6) may be an independent prognostic biomarker for nasopharyngeal carcinoma (NPC). PMID: 27623076
    5. We identified a novel SNP variant, Chr12:g.53494591T>C. c.T430C (p.S144P) in the insulin-like growth factor binding protein-6 (IGFBP6) gene. This variant was shared by all affected family members, but not by unaffected members. The c.T430C (p.S144P) variant of IGFBP6 was identified as the likely causal variant associated with increased risk of familial disc degeneration in the studied pedigree. PMID: 28829625
    6. We found that IGFBP6 and SATB2 were significantly down-regulated in HIV-infected CEM*174 cells and 3 different cohorts of HIV/AIDS patients while their promoters were predominantly hyper-methylated compared with normal controls. PMID: 26039376
    7. IGFBP6 attenuation in ACTH-secreting pituitary adenomas is associated with tumor growth, through activation of PI3K-AKT-mTOR pathway PMID: 24379119
    8. CCL-18 and IGFBP-6 were identified as new potential serum biomarkers for prostate cancer. PMID: 24747338
    9. When the concentrations of IGFBP-6 or KNG1 were greater than 98.5 pg/ml or 88.5 ng/ml, respectively, they predicted the proliferative vitreoretinopathy prognosis. PMID: 23808406
    10. Data indicate that IGFBP-6 binds to prohibitin-2 on the cell membrane, and knockdown of the latter abrogates IGFBP-6-induced migration. PMID: 24003225
    11. Review of the IGF system in physiology and disease with a focus on the regulation and actions of IGFBP-6, and its potential roles in cancer cells. PMID: 23126425
    12. These results suggest that the expression of IGFBP-6 in vascular endothelial cells is up-regulated by hypoxia and IGFBP-6 inhibits angiogenesis in vitro and in vivo PMID: 21618524
    13. IGFBP-6 acts as a inhibitory mediator of thyroid hormone effects in osteoblast differentiation PMID: 21997736
    14. TCDD at low concentrations may have a negative effect on cell apoptosis and down-regulate gene expression of IGFBP-6 in SaOS-2 cells. PMID: 18724896
    15. Hh pathway is aberrant activation in colorectal carcinoma cell line. Its inhibitor may be effectual agent for colorectal cancer chemoprevention. Gli1 maintained cell survival by binding promoter regions and facilitating transcription of IGFBP6 and Bcl-2. PMID: 21940310
    16. When IGFBP-6 gene expression was downregulated, cell proliferation was inhibited and apoptotic cell death was increased. PMID: 21820463
    17. Kininogen-1 and IGFBP-6 are expressed in serum and vitreous humor in proliferative vitreoretinopathy patients. PMID: 21054968
    18. IGFBP-6 inhibits osteoblastic differentiation mediated by vitamin d3 by directly binding the vitamin d receptor and inhibiting its function PMID: 21458526
    19. These results indicate that IGFBP-6 promotes Rh30 rhabdomyosarcoma chemotaxis in an IGF-independent manner, and that MAPK signaling pathways and their cross-talk play an important role in this process. PMID: 20432455
    20. Inhibition of human osteoblast marker gene expression by retinoids is mediated in part by insulin-like growth factor binding protein-6. PMID: 11914024
    21. may function as an antiproliferative molecule suppressing mitogenic effects of insulin-like growth factors on Malassez cells PMID: 12558805
    22. CCI-779 acts additively with IGFBP-6 to reduce rhabdomyosarcoma growth both in vitro and in vivo. PMID: 14710364
    23. C-domain of IGFBP-6 consists of a thyroglobulin type 1 fold comprising an alpha-helix followed by a loop, a three-stranded antiparallel beta-sheet incorporating a second loop, and a disulfide-bonded flexible third loop. PMID: 15308688
    24. A close correlation exists between residues of the carboxyl-terminal domain of IGFBP6 undergoing conformational change in 15N NMR spectroscopy studies with those that disappeared or broadened upon insulin-like growth factor (IGF-II) binding. PMID: 15366928
    25. The IGFBP-6 protein is synthesized as propeptides with a hydrophobic leader sequence, removal of which yields a mature protein composed of 3 recognizable domains of similar size. PMID: 15797461
    26. transgenic human IGFBP-6 mice may be considered a new tool for studies of the involvement of the brain IGF system in metabolism control and obesity. PMID: 15889232
    27. p38 MAPK is involved in IGFBP-6-induced IGF-independent rhabdomyosarcoma cell migration PMID: 17519236
    28. Toxic effect of TCDD on osteogenesis through altering IGFBP6 gene expression in osteoblasts is reported. PMID: 17978469
    29. IGFBP-6 is translocated to the nucleus with functional consequences, and different members of the IGFBP family have specific nuclear import mechanisms. PMID: 18039785
    30. These studies provide evidence that overexpression of IGFBP-6 suppresses human and murine osteoblast differentiation, that IGFBP-6 and LMP-1 physically interact, and supports the conclusion that this interaction may be functionally relevant. PMID: 18395833
    31. bexarotene increased the occupancy of the identified enhancer element in IGFBP-6 gene by RXRalpha, RARbeta, cJun, cFos, and p300 PMID: 18957410
    32. Data show that an increase in JNK activation in the presence of NFkappaB inhibition significantly increased the expression of IGFBP6. PMID: 18982452
    33. IGFBP-6 is the effector of tumor suppressor activity of SEMA3B. PMID: 18985860

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  • 亞細胞定位：
    Secreted.
  • 數據庫鏈接：

    HGNC: 5475

    OMIM: 146735

    KEGG: hsa:3489

    STRING: 9606.ENSP00000301464

    UniGene: Hs.274313



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