在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Homeobox protein engrailed-2(EN2) ELISA kit

  • 中文名稱:
    人鋸齒狀同源異型盒蛋白2(EN2)酶聯免疫試劑盒
  • 貨號:
    CSB-EL007660HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人鋸齒狀同源異型盒蛋白2(EN2)酶聯免疫試劑盒(CSB-EL007660HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EN2含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產品適用于體外研究EN2蛋白在疾病模型中的表達變化,如腫瘤生物學機制探索、基因功能研究或藥物干預效果評估等領域,為科研人員提供穩定可靠的分析工具,助力于癌癥相關分子標記物篩選及分子通路研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    EN2 ELISA Kit; Homeobox protein engrailed-2 ELISA Kit; Homeobox protein en-2 ELISA Kit; Hu-En-2 ELISA Kit
  • 縮寫:
    EN2
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Epigenetics and Nuclear Signaling
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human EN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %92
    Range %86-96
    1:4Average %97
    Range %96-105
    1:8Average %94
    Range %89-98
  • 回收率:
    The recovery of human EN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8380-87
    EDTA plasma (n=4)9286-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.466 2.365 2.416 2.271
    101.759 1.658 1.709 1.564
    51.028 1.007 1.018 0.873
    2.50.615 0.604 0.610 0.465
    1.250.391 0.402 0.397 0.252
    0.6250.258 0.268 0.263 0.118
    0.3120.221 0.224 0.223 0.078
    00.144 0.145 0.145  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 基因功能參考文獻:
    1. The EN2 transcription factor is a novel ovarian cancer biomarker. It demonstrates prognostic value, correlating with worse Overall Survival and Progression-free Survival. PMID: 30285763
    2. we confirmed that engrailed-2 is the target of hsa-miR-27b in human cytomegalovirus-infected glioma cells PMID: 28343438
    3. The critical role of miR212 in inhibiting prostate tumor growth was verified in xenograft models of prostate cancer. These findings highlighted the role of miR212 in PCa progression. More importantly, we speculate that EN-2 is a direct target gene of miR212. PMID: 28713997
    4. We identified a novel heterozygous missense variant (c.425C>G, p.Pro142Arg) in the Engrailed 2 (EN2) gene in two patients. The G variant has never been reported in public databases and was absent in 100 Thai patients with ASD and 435 Thai controls. A case-control study showed that the G allele of c.425C>G was significantly associated with ASD PMID: 27755371
    5. EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer (PCa) cells by up-regulating EN2, which leads to PCa development and progression PMID: 28943214
    6. Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer PMID: 27921232
    7. the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells. PMID: 26948025
    8. EN2 may be a candidate oncogene in bladder cancer by activating the PI3K/Akt pathway and inhibiting PTEN. PMID: 25812440
    9. Engrailed-2 (EN-2) is a developmentally regulated homeobox gene that is essential for Purkinje cell maturation and normal cerebellar development and patterning. PMID: 25290267
    10. The mean EN2 levels in prostate cancer patients were 3.76-fold higher than those in non-prostate patients after prostate massage. PMID: 25003607
    11. The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation. PMID: 24532139
    12. En2 identified as a new regulator of T-cell differentiation whose disruption induces a malignant T-cell fate in chronic myelogenous leukemia progression. PMID: 24675889
    13. Two patients with underdeveloped arcuate fasciculus had novel, nonsynonymous variants in MID1 and EN2 genes regulating axon guidance pathway. PMID: 24321989
    14. EN2 levels are increased in affected individuals with an A-C/G-T genotype, supporting EN2 as an autism spectrum disorder susceptibility gene. PMID: 24520327
    15. urinary EN2 has the potential to be a more sensitive and specific protein biomarker for non-muscle-invasive bladder cancer than currently available tests. PMID: 23434148
    16. Urinary EN2 may be useful as a non-invasive early biomarker for prostate cancer detection in genetically high-risk individuals PMID: 23792811
    17. Normal EN-2 downregulation may not occur in some individuals with autism, such that the postnatal persistence of EN-2 overexpression may contribute to autism cerebellar abnormalities. PMID: 23423141
    18. The WNT2 gene and EN2 gene may act in concert to influence the language development in autism. PMID: 22522212
    19. findings show EN2 was expressed and secreted by prostate cancer (PC) cell lines and PC tissue but not by normal prostate tissue or stroma; presence of EN2 in urine was highly predictive of PC PMID: 21364037
    20. This study demonistrated that in Han chinese the association between the EN2 genetic polymorphism and autism. PMID: 20523082
    21. Positive genetic correlation of EN2 with autism in the Indian population. PMID: 20050924
    22. In human, failure to form a mesencephalon and metencephalon, with cerebellar hypoplasia, results drom a mutation or deletion in EN2(Engrailed-2) gene. PMID: 11815869
    23. EN2 exon 1 variant transmission disequilibrium test shows no association with autistic disorder nor association between language and stereotypic behaviour quantitative traits. PMID: 12525552
    24. An association between a cerebellar patterning gene and autism spectrum disorder suggests a role for EN2 as a susceptibility locus and supports a neurodevelopmental defect hypothesis in the etiology of autism. PMID: 15024396
    25. RNA interference studies suggest that EN2 expression is required for the maintenance of the transformed phenotype of a human breast tumor cell line PMID: 16007149
    26. Loss and gain of function experiments firmly establish that En1/2 is a true survival factor for dopaminergic neurons in vivo. PMID: 17267560
    27. Heterogeneous association between engrailed-2 and autism in the CPEA network PMID: 17948868
    28. a preferential transmission of the rs3824068 A-allele to AUTISM affected offspring PMID: 17948901
    29. Engrailed 2 gene is associated with autistic disorder in Chinese children. PMID: 18424904
    30. The majority of regulated genes were down-regulated in engrailed-2 cerebella from transgenic mice, consistent with the known repressive function of Engrailed-2. PMID: 18562208
    31. variations of the engrailed-2 gene are implicated in the development of young-onset Parkinson's disease PMID: 19270442
    32. The data of this study indicated that the A-C haplotype is functional and, together with the association and LD mapping results, supports EN2 as a likely autism susceptibility gene and the A-C haplotype as a possible risk allele. PMID: 19615670

    顯示更多

    收起更多

  • 相關疾病:
    Genetic variations in EN2 may be associated with susceptibility to autism.
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    Engrailed homeobox family
  • 數據庫鏈接:

    HGNC: 3343

    OMIM: 131310

    KEGG: hsa:2020

    STRING: 9606.ENSP00000297375

    UniGene: Hs.134989



主站蜘蛛池模板: 夜夜国产亚洲视频香蕉| 自拍偷自拍亚洲精品牛影院| 乱中年女人伦av三区| 久久精品国产99久久久古代| 国产精品美女乱子伦高| 久久人午夜亚洲精品无码区| 九九视频免费精品视频| 久久精品99久久香蕉国产色戒| 日本午夜免费啪视频在线| 国产超碰女人任你爽| 粗大的内捧猛烈进出视频| 女人爽到高潮免费看视频| 欧美孕妇变态孕交粗暴| 三级无码在钱av无码在钱| 精品无码国产污污污免费| 色妞色视频一区二区三区四区| 国产亚洲精品福利在线无卡一| 亚洲中文精品久久久久久| 噜噜噜亚洲色成人网站∨| 成人国产欧美大片一区| 亚洲国产成人久久久网站 | 97精品国产一区二区三区四区 | 国产区精品福利在线社区| 国产精品熟女高潮视频| 亚洲国产精品久久久天堂麻豆宅男| 久久精品国产精品亚洲38| 男人添女人囗交做爰视频| 久久精品中文闷骚内射| 少妇一边呻吟一边说使劲| av天堂亚洲区无码先锋影音| 性做久久久久久免费观看| 国产精品无码综合区| 人妻熟女久久久久久久| 久久av无码αv高潮αv喷吹| 玩弄人妻少妇500系列网址| 日本高清在线天码一区播放| 精品一区二区三区在线视频| 中文字幕丰满乱孑伦无码专区| 国产毛片不卡野外视频| 日韩免费无码专区精品观看| 国产免费内射又粗又爽密桃视频|