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Human Glutaminyl-peptide cyclotransferase(QPCT) ELISA kit

  • 中文名稱:
    人谷氨酰胺酰肽環轉移酶(QPCT)酶聯免疫試劑盒
  • 貨號:
    CSB-EL019135HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人谷氨酰胺酰肽環轉移酶(QPCT)酶聯免疫試劑盒(CSB-EL019135HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的QPCT含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于基礎科研中QPCT蛋白表達水平檢測,例如細胞模型或動物模型中酶活性調控機制研究、疾病相關生物標志物探索以及藥物干預后分子通路變化的評估,為神經科學、腫瘤學及代謝疾病領域的分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    5730422A13Rik ELISA Kit; EC ELISA Kit; GCT ELISA Kit; Glutaminyl cyclase ELISA Kit; Glutaminyl peptide cyclotransferase ELISA Kit; Glutaminyl tRNA cyclotransferase ELISA Kit; Glutaminyl-peptide cyclotransferase ELISA Kit; Glutaminyl-tRNA cyclotransferase ELISA Kit; Glutamyl cyclase ELISA Kit; QC ELISA Kit; qpct ELISA Kit; QPCT_HUMAN ELISA Kit; sQC ELISA Kit
  • 縮寫:
    QPCT
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human QPCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 92
    Range % 87-100
    1:2 Average % 103
    Range % 99-105
    1:4 Average % 93
    Range % 87-97
    1:8 Average % 91
    Range % 89-98
  • 回收率:
    The recovery of human QPCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 92-98
    EDTA plasma (n=4) 90 86-94
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 1.846 1.775 1.811 1.676
    10 1.469 1.466 1.468 1.333
    5 1.156 1.244 1.200 1.065
    2.5 0.878 0.854 0.866 0.731
    1.25 0.583 0.623 0.603 0.468
    0.625 0.374 0.388 0.381 0.246
    0.312 0.242 0.251 0.247 0.112
    0 0.134 0.135 0.135  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.
  • 基因功能參考文獻:
    1. The addition of QC activity to core diagnostic CSF biomarkers may be of specific interest in clinical cases with discordant imaging and biochemical biomarker results. PMID: 28587659
    2. these findings demonstrate a significant association between common single nucleotide polymorphism within QPCT gene and schizophrenia risk in a Han Chinese population. PMID: 26572640
    3. the genetic risk of QPCT gene for schizophrenia also exists in the Han Chinese population. PMID: 26492838
    4. observations provide evidence for an involvement of QC in Alzheimer's disease pathogenesis and cognitive decline by QC-catalyzed pGlu-Abeta formation PMID: 24164736
    5. this study demonistrated that QPCT mRNAand protein from mononuclear cells correlated with theseverity of dementia. PMID: 23207485
    6. Upregulation of QPCT expression is associated with thyroid carcinomas. PMID: 23183267
    7. This study presents a first comparison of two mammalian QCs (human and mouse) containing typical, conserved post-translational modifications. PMID: 21671571
    8. The resilts of this studt provided histopathological evidence for QC being a prerequisite for pE-Abeta pathology in vivo and further underline the therapeutic potential of QC inhibition in Alzheimer Disease. PMID: 21301857
    9. Upon binding to PBD150, a large loop movement in gQC allows the inhibitor to be tightly held in its active site primarily by hydrophobic interactions. PMID: 21288892
    10. This study demonstrated that glutaminyl cyclase expression and pE-Abeta formation in subcortical brain regions( Edinger-Westphal nucleus, locus coeruleus and nucleus basalis Meynert) affected in Alzheimer's disease. PMID: 20383514
    11. Human isoQC proteins displayed a broad substrate specificity and preference for hydrophobic substrates, similar to the related QC. PMID: 19804409
    12. Functional expression of human glutaminyl cyclase in Pichia pastoris reveals the presence of a disulfide bond with an important role in the stabilization of active protein structure. PMID: 12196024
    13. human glutaminyl cyclase is a metal-dependent transferase PMID: 14522962
    14. Glutaminyl cyclase was expressed as a fully active 37 kDa enzyme with kcat/Km values of 14.3, 9.3, and 2.4 mM(-1)s(-1) for the substrates Gln-Gln, Gln-NH(2), and Gln-t-butyl ester, respectively PMID: 14680951
    15. Genetic variations in QPCT are important factors affecting the bone mineral density of adult women that contribute to susceptibility for osteoporosis. PMID: 15231017
    16. structure of human QC in free form and bound to a substrate and three imidazole-derived inhibitors PMID: 16135565
    17. Our results suggest that QPCT may be the QTL gene at chromosome 2p for spine BMD variation in the Chinese population PMID: 17687619
    18. combined steady-state enzyme kinetic and X-ray structural analyses of 11 single-mutation human QCs to investigate the roles of the H-bond network in catalysis PMID: 18072935
    19. The computations outline the important role of Trp329 in helping the substrate binding process and stabilizing the cyclization transition state. PMID: 18470930
    20. Golgi apparatus retention implies a "housekeeping" protein maturation machinery conducting glycosylation and pyroglutamyl formation. For these isoenzymes, apparently similar strategies evolved to retain the proteins in the Golgi complex. PMID: 18486145

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Glutaminyl-peptide cyclotransferase family
  • 數據庫鏈接:

    HGNC: 9753

    OMIM: 607065

    KEGG: hsa:25797

    STRING: 9606.ENSP00000344829

    UniGene: Hs.79033



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