在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Elafin(PI3) ELISA kit

  • 中文名稱:
    人彈性蛋白酶抑制劑(PI3)酶聯免疫試劑盒
  • 貨號:
    CSB-EL017952HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人彈性蛋白酶抑制劑(PI3)酶聯免疫試劑盒(CSB-EL017952HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PI3含量。PI3信號通路是一種細胞內信號轉導途徑,響應細胞外信號,調節多種細胞功能,如代謝、增殖、細胞存活、生長和血管生成等。其研究機制涉及PI3K(磷脂酰肌醇3-激酶)和AKT(蛋白激酶B)等關鍵基因,通過磷酸化下游靶標調控細胞生長、存活和凋亡等生命活動。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,靈敏度為1.95 pg/mL。該產品廣泛應用于基礎醫學研究領域,如炎癥相關信號通路解析、蛋白酶 - 抗蛋白酶系統平衡機制探索,以及疾病模型中 PI3 動態變化的定量分析,為呼吸系統疾病、免疫調節及組織損傷修復等方向的科研工作提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cementoin ELISA Kit; ELAF_HUMAN ELISA Kit; Elafin ELISA Kit; Elafin/Skalp ELISA Kit; Elastase Specific Inhibitor ELISA Kit; Elastase-specific inhibitor ELISA Kit; ES 1 ELISA Kit; ESI ELISA Kit; Peptidase inhibitor 3 ELISA Kit; Peptidase inhibitor 3; skin derived ELISA Kit; PI 3 ELISA Kit; PI-3 ELISA Kit; PI3 ELISA Kit; Pre elafin ELISA Kit; Protease inhibitor 3 skin derived ELISA Kit; Protease inhibitor 3; skin derived (SKALP) ELISA Kit; Protease inhibitor WAP3 ELISA Kit; SKALP ELISA Kit; Skin derived Anti leukoproteinase ELISA Kit; Skin-derived antileukoproteinase ELISA Kit; Trappin 2 ELISA Kit; WAP four disulfide core domain 14 ELISA Kit; WAP four disulfide core domain protein 14 ELISA Kit; WAP four-disulfide core domain protein 14 ELISA Kit; WAP3 ELISA Kit; WFDC14 ELISA Kit
  • 縮寫:
    PI3
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    7.8 pg/mL-500 pg/mL
  • 靈敏度:
    1.95 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    62.974

    63.991

    62.090

    61.964

    63.482

    61.586

    SD

    0.042

    0.035

    0.037

    0.052

    0.047

    0.050

    CV(%)

    5.364

    4.425

    4.768

    6.710

    5.972

    6.477

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:20

    Average %

    89

    Range %

    85-93

    1:40

    Average %

    100

    Range %

    95-105

    1:80

    Average %

    87

    Range %

    83-91

    1:160

    Average %

    105

    Range %

    101-109

  • 回收率:

    The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    86

    83-89

    EDTA plasma (n=4)

    99

    95-104

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.783

    2.901

    2.842

    2.678

    250

    1.852

    1.798

    1.825

    1.661

    125

    1.210

    1.205

    1.208

    1.044

    62.5

    0.779

    0.789

    0.784

    0.620

    31.2

    0.483

    0.478

    0.481

    0.317

    15.6

    0.331

    0.354

    0.343

    0.179

    7.8

    0.279

    0.268

    0.274

    0.110

    0

    0.165

    0.163

    0.164

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1).
  • 基因功能參考文獻:
    1. Elafin decreased in active patients with inflammatory bowel disease and was negatively correlated with disease activity, suggesting that elafin may play a protective role PMID: 29084078
    2. Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome PMID: 28187039
    3. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
    4. Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia PMID: 28802561
    5. significantly higher expression in preterm prelabor ruptured fetal membranes than in membranes from term birth PMID: 27580179
    6. Our study revealed that high elafin levels identified in smoking- and asthma-related microarray data sets and an epidemiologic study significantly reduced the risk of asthma. PMID: 26932165
    7. The expression of elafin in fallopian tubes of ectopic pregnancies is reduced. PMID: 25356943
    8. The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor. PMID: 25559229
    9. PI3 expression was downregulated in 24% of DCIS and 83% of invasive breast tumors. PI3 was also downregulated in 33% of ovarian cystadenomas, 43% of borderline ovarian tumors, and 86% of invasive ovarian carcinomas. PMID: 25551582
    10. The imbalance between neutrophil elastase and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer. PMID: 25195861
    11. Higher urinary elafin levels are associated with an increased risk of micro- and macroalbuminuria, acute kidney injury and chronic kidney disease, and death after hematopoietic stem cell transplantation. PMID: 25388519
    12. cutaneous elafin does not seem to discriminate acute graft-versus-host disease from drug hypersensitivity rash lesions, but there may be an association between cutaneous elafin expression and poor prognosis for patients with cutaneous GVHD PMID: 25405322
    13. Data indicate that elafin overexpression is associated with poor overall survival. PMID: 24469047
    14. Low expression of elafin gene correlated with significantly reduced time to relapse, and when combined with high expression of elastase gene was associated with decreased survival in breast cancer patients PMID: 23320734
    15. Elafin has important roles in normal homoeostasis and at sites of inflammation. These roles include antiprotease and antimicrobial activity. PMID: 19906197
    16. Results confirm the association between SNP rs2664581 and enhanced risk of developing acute respiratory distress syndrome. PMID: 24617927
    17. Tubal epithelium from women with hydrosalpinx has lower levels of elafin expression. PMID: 23885101
    18. Elafin expression in the small intestinal epithelium was lower in patients with active celiac disease compared with control patients. In vitro, elafin significantly slowed the kinetics of the deamidation of the 33-mer peptide to its more immunogenic form. PMID: 24710505
    19. Production of HBD2 and elafin by vaginal epithelial cells is sensitive to E, suggesting that innate immune protection varies in the vagina across the menstrual cycle. PMID: 23398087
    20. Elafin expression may establish the diagnosis of pre-engraftment syndrome and graft versus host disease. PMID: 23866880
    21. Trappin-2 and elafin significantly reduce HSV-2 viral attachment to, and replication in genital epithelial cells. PMID: 23637403
    22. The N-terminus of elafin is critical for intranuclear localization and anti-HIV-1 activity. PMID: 23300756
    23. Studies indicate that Trappin-2/Elafin possessing anti-microbial properties against different classes of pathogens including viruses, fungi and bacteria. PMID: 22634606
    24. The results suggest that decreased expression of elastin and LOX and increased expression of elafin in the cardinal ligaments may contribute to pelvic organ prolapse. PMID: 19087518
    25. The endometrium of women with hydrosalpinx has an increased number of neutrophils and lower expression of elafin, an elastase inhibitor and natural antimicrobial molecule. PMID: 21392745
    26. these results collectively suggest that, in melanoma cells, Foxa2 expression is silenced and therefore elafin is maintained unexpressed to facilitate cell proliferation in the disease melanoma. PMID: 21466784
    27. ability to modulate the expression of some P.aeruginosa virulence factors PMID: 20932308
    28. These results suggest that elafin can maintain airway epithelium integrity by protecting airway epithelial cells and enhancing the anti-inflammatory capability of airway. PMID: 19823954
    29. increased production at the secretory phase of the menstrual cycle relative to the proliferative phase PMID: 19824918
    30. Elafin has a role in graft-versus-host disease of the skin PMID: 20371463
    31. Elafin may serve as a determinant of poor survival in serous ovarian carcinomas PMID: 20126474
    32. Overexpression of elafin in epithelial cells attenuated the damage of Pseudomonas aeruginosa biofilm. PMID: 19420899
    33. Elafin is a component of cervicovaginal secretions in pregnancy, and levels are diminished in bacterial vaginosis. PMID: 19723838
    34. Synthesized locally at mucosal sites. Efficiently inhibit target enzymes released into interstitium. Might be important in controlling excessive neutrophil elastase release in chronic obstructive pulmonary disease and cystic fibrosis. (review) PMID: 11667971
    35. regulation of the elafin gene is dependent on chromatin structure in keratinocytes PMID: 12542536
    36. results indicate that elafin may have a dual function, promoting up-regulation of local lung innate immunity while simultaneously down-regulating potentially unwanted systemic inflammatory responses in the circulation PMID: 12819058
    37. elafin regulates proteolytic enzymes during menstruation and contributes to the innate defense against uterine infection PMID: 12970320
    38. Serine elastase inhibition (elafin overexpression) appears to suppress inflammation, cardiac dilatation, and dysfunction after myocardial infarct. PMID: 14693682
    39. Gene augmentation of human elafin suggests a novel and extended anti-inflammatory role for this human neutrophil elastase inhibitor working as an effector of innate immunity to protect tissues against maladaptive inflammatory responses. PMID: 15034071
    40. The differential biological activity of elafin in different conditions suggests a role for this molecule in either LPS detoxification or activation of innate immune responses, depending on the external cellular environment. PMID: 15668324
    41. Oxidized elafin poorly inhibits the elastolytic activity of leukocyte elastase and proteinase 3. PMID: 16279952
    42. transglutaminase 2-mediated cross-linking of serine protease inhibitors elafin and trappin-2 to neutrophil serine proteinases preserves their inhibitory capacities PMID: 16300411
    43. The pleiotropic molecule elafin has significant potential in modulating antigen-presenting cell numbers and activity, and could be beneficial in mucosal protective strategies. PMID: 16424380
    44. The promoter sequences of PI3 have a high degree of variability. PMID: 16719916
    45. beneficial effects of pre-elafin could be mediated, at least in part, by its ability to increase G-CSF levels in the lung PMID: 16913840
    46. The transglutaminase substrate moiety of elafin plays an important role in anchoring elafin at its proper sites of action during UV-induced aging processes. PMID: 17139263
    47. Levels of mRNA and immunostaining of the antiproteases elafin and SLPI were enhanced strongly in inflamed versus noninflamed UC. Elafin and SLPI may be added to the list of defensin-like peptides with diminished induction in CD versus UC. PMID: 17200145
    48. full antipeptidase activity of pre-elafin is essential to protect against lung tissue lesions PMID: 17489739
    49. This review focuses on recent findings revealing that elafin has many biological functions as diverse as anti-bacterial, anti-fungal, anti-viral, anti-inflammatory and immuno-modulatory functions. PMID: 17964057
    50. We report here that pre-elafin inhibits a secreted peptidase to prevent Pseudomonas aeruginosa growth in vitro. PMID: 18025118

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:

    HGNC: 8947

    OMIM: 182257

    KEGG: hsa:5266

    STRING: 9606.ENSP00000243924

    UniGene: Hs.112341



主站蜘蛛池模板: 欧美亚洲另类自拍丝袜| 久久超碰精品一夜七次郎| 国产做a爰片久久毛片a片白丝| 国产福利一区二区麻豆| 夜精品a片一区二区三区无码白浆 漂亮人妻被中出中文字幕 | 国产尤物精品自在拍视频首页| 97久久人人超碰超碰窝窝| 国产一区二区三区乱码| 成人性生交大片免费看 | 亚洲h成年动漫在线观看网站| 人妻熟女一区二区aⅴ清水理纱 | 国产精品va在线观看无码| 天堂а√中文最新版在线| 国产超碰人人模人人爽人人喊| 巨乳美乳一区二区三区| 2021国产精品自在自线| 欧美色欧美亚洲另类二区| 无码国模大尺度视频在线观看| 日韩99在线 | 中文| 国产精品福利自产拍久久| 天堂网在线最新版www资源网| 特黄做受又粗又长又大又硬| 国产传媒精品1区2区3区| 国产精品福利一区二区| 欧洲熟妇牲交| 亚洲欧洲无码av不卡在线| 2019一級特黃色毛片免費看| 97久久超碰亚洲视觉盛宴| 成人麻豆日韩在无码视频| 动漫成人无码精品一区二区三区| 野外做受又硬又粗又大视频√| 特级av毛片免费观看| 成年午夜性影院免费观看| 97se亚洲精品一区二区 | 国产卡一卡二卡三| av无码久久久久久不卡网站| 吃奶摸下高潮60分钟免费视频| 日本大香伊一区二区三区| 我的公把我弄高潮了视频| 欧美制服丝袜人妻另类| 一边捏奶一边高潮视频|