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  4. Human Dickkopf-related protein 2(DKK2) ELISA kit

Human Dickkopf-related protein 2(DKK2) ELISA kit

  • 中文名稱：
    人Dickkopf相關(guān)蛋白2(DKK2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006921HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Dickkopf相關(guān)蛋白2(DKK2)酶聯(lián)免疫試劑盒（CSB-EL006921HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的DKK2含量。DKK2，全稱dickkopf WNT signaling pathway inhibitor 2，是一種分泌蛋白，能夠抑制β-聯(lián)蛋白介導(dǎo)的Wnt信號傳導(dǎo)，并具有促血管生成活性。其在多種腫瘤組織中表達上調(diào)，通過抑制殺傷性免疫細胞激活和減弱腫瘤血管生成來抑制腫瘤形成。研究顯示，DKK2的抑制作用與CD8+細胞毒性T淋巴細胞(CTL)活性和腫瘤血管生成減弱有關(guān)，是治療癌癥的有希望靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于體外培養(yǎng)細胞模型、動物組織或體液樣本的DKK2蛋白定量研究；為探索Wnt信號通路在癌癥、骨疾病、干細胞分化等領(lǐng)域的分子機制提供可靠工具，滿足基礎(chǔ)科研中對蛋白表達動態(tài)分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Dickkopf 2 ELISA Kit; Dickkopf gene 2 ELISA Kit; Dickkopf homolog 2 ELISA Kit; Dickkopf related protein 2 ELISA Kit; Dickkopf WNT signaling pathway inhibitor 2 ELISA Kit; Dickkopf-2 ELISA Kit; Dickkopf-related protein 2 ELISA Kit; Dickkopf2 ELISA Kit; DKK 2 ELISA Kit; Dkk-2 ELISA Kit; Dkk2 ELISA Kit; DKK2_HUMAN ELISA Kit; hDkk 2 ELISA Kit; hDkk-2 ELISA Kit; hDkk2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DKK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %88-98
    1:2Average %86
    Range %80-94
    1:4Average %96
    Range %92-102
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of human DKK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)9895-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.416 2.456 2.436 2.324
    101.579 1.685 1.632 1.520
    50.834 0.901 0.868 0.756
    2.50.515 0.545 0.530 0.418
    1.250.372 0.365 0.369 0.257
    0.6250.266 0.255 0.261 0.149
    0.3120.184 0.178 0.181 0.069
    00.113 0.111 0.112 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 基因功能參考文獻：
    1. our findings demonstrate that DKK2 functions as a tumor suppressor through inhibiting cell proliferation and inducing apoptosis via regulating Wnt signaling during breast tumorigenesis. PMID: 28467796
    2. Knock down of the dickkopf WNT signaling pathway inhibitor 2 (DKK2) resulted in a significant suppression of HOXD10, HOXD11 and HOXD13 while over-expression of DKK2 and stimulation with factors of the WNT signaling pathway. PMID: 27363011
    3. epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival. PMID: 28152305
    4. the genotyping and functional findings are consistent with the hypothesis that DKK2 is a tumor suppressor; by selectively retaining a transcriptionally inactive DKK2 allele, the reduction of DKK2 function results in unchecked Wnt/beta-catenin signaling, contributing to hepatocellular carcinoma oncogenesis. PMID: 27203079
    5. The present report suggested that DKK2 downregulation suppressed the proliferation and invasion of prostate cancer cells by inhibiting the Wnt/betacatenin signaling pathway. PMID: 27431620
    6. these findings reveal that the miR-187-5p-DKK2 pathway regulates Wnt/beta-catenin signaling, cell growth, and apoptosis. Our findings provide the first evidence of a role for miR-187-5p in promotion of B-cell ALL. PMID: 27296949
    7. Genetic variant in DKK2 gene is associated with gallbladder cancer. PMID: 26715268
    8. targeting of DKK2 by miR-154 leads to upregulation of beta-catenin expression and activation of the classical Wnt signaling pathway and cardiac fibroblasts. PMID: 27542661
    9. MiR-21 overexpression was significantly correlated with the DKK2-/beta-catenin- immunohistochemical phenotype. PMID: 23999978
    10. Data indicate that inhibiting dickkopf-related protein 2 (DKK2)with siDKK2 reduced the expression of DKK2, but had no effect on leptin expression. PMID: 25312721
    11. We found 1 DKK2 SNP to be significantly associated with alcohol-related harm in alcoholic subjects PMID: 24117450
    12. a new regulatory mechanism of miR-222 PMID: 23587485
    13. Our results provide strong evidence that DKK2 is a key player in Ewing sarcoma invasion and osteolysis and also in the differential phenotype of Ewing sarcoma cells. PMID: 23204234
    14. DKK2 may contribute to tumorigenesis in epithelial ovarian cancer(EOC) through the Wnt/beta-catenin signaling mechanisms. PMID: 22964660
    15. Elevated TGF-beta1 levels in osteoarthritic osteoblasts can stimulate DKK2 expression. PMID: 21312269
    16. In the study of the genetics of alcoholism, three SNPs in DKK2 (rs427983; rs419558; rs399087) demonstrated empirical significance. PMID: 20332099
    17. DKK2 is epigenetically silenced by methylation in higher grades and stages of renal cell carcinoma; results suggest that DKK2 inhibits renal cancer progression through apoptotic and cell cycle pathways PMID: 19755393
    18. Analysis of individual Dkk domains and chimeric Dkks shows that the carboxy-terminal domains of both Dkk1 & 2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. PMID: 12167704
    19. analysis of the structural basis of the interaction between Dkk and low density lipoprotein receptor-related protein (LRP) 5/6 PMID: 18524778

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Dickkopf family
  • 組織特異性：
    Expressed in heart, brain, skeletal muscle and lung.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2892

    OMIM: 605415

    KEGG: hsa:27123

    STRING: 9606.ENSP00000285311

    UniGene: Hs.211869



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