在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Carbonic anhydrase 1(CA1) ELISA kit

  • 中文名稱:
    人碳酸酐酶1(CA1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL004364HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人碳酸酐酶1(CA1)酶聯免疫試劑盒(CSB-EL004364HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CA1含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL,靈敏度為0.78 pg/mL。適用于科研領域中對CA1在腫瘤微環境、代謝調控或病理生理機制中的功能研究,也可用于探索其作為潛在生物標志物的應用價值。為體外研究CA1的生物學作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CA 1 ELISA Kit; CA I ELISA Kit; CA-I ELISA Kit; CA1 ELISA Kit; CAB ELISA Kit; CAH1_HUMAN ELISA Kit; CAI ELISA Kit; CAN ELISA Kit; Car 1 ELISA Kit; Car1 ELISA Kit; Carbonate dehydratase I ELISA Kit; Carbonic anhydrase 1 ELISA Kit; Carbonic anhydrase A ELISA Kit; Carbonic anhydrase B ELISA Kit; Carbonic anhydrase B; formerly ELISA Kit; Carbonic anhydrase I ELISA Kit; Carbonic dehydratase ELISA Kit; ECK0125 ELISA Kit; JW0122 ELISA Kit; yadF ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    98

    Range %

    91-105

    1:2

    Average %

    87

    Range %

    83-93

    1:4

    Average %

    95

    Range %

    88-102

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of human CA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    100

    96-106

    EDTA plasma (n=4)

    95

    88-101

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.147

    2.134

    2.140

    1.959

    100

    1.588

    1.667

    1.627

    1.446

    50

    0.969

    1.028

    0.998

    0.817

    25

    0.646

    0.629

    0.637

    0.456

    12.5

    0.433

    0.421

    0.427

    0.245

    6.25

    0.311

    0.323

    0.317

    0.136

    3.12

    0.252

    0.244

    0.248

    0.067

    0

    0.178

    0.185

    0.181

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
  • 基因功能參考文獻:
    1. Anti-CA I and II antibody levels were investigated using ELISA in serum samples from 30 patients with Acute Myeloid Leukemia (AML) and 30 healthy peers. Anti-CA I and II antibody titers in the AML group were significantly higher compared with the control group (p=0.0001 and 0.018, respectively). PMID: 28270370
    2. These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively PMID: 28613396
    3. These results showed that flavonoids especially malvin and oenin effectively inhibited hCA I and II isoenzymes. PMID: 28445001
    4. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base. PMID: 28544359
    5. Here are presented solved the first crystal structures of carbonic anhydrase 1and carbonic anhydrase 2 in complex with polmacoxib, at 2.0 A and 1.8 A, respectively. PMID: 27475498
    6. Neuronal carbonic anhydrase I (CA1) appears to be associated with the endoplasmic reticulum subcellular structure. PMID: 27809276
    7. CA1 is highly expressed in the sera of stage I non-small cell lung cancer patients PMID: 26232327
    8. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on Carbonic Anhydrase activity. PMID: 27413740
    9. CA1 is a potential oncogene and that it contributes to abnormal cell calcification, apoptosis and migration in breast cancer. PMID: 26459317
    10. We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
    11. The gene encoding CA1 is susceptible to ankylosing spondylitis and plays a role in the process of bone formation. PMID: 22838845
    12. Structure-activity relationships study showed that indolylchalcone derivatives have higher inhibitory activities than pyrido[2,3-d]pyrimidine derivatives on hCA I and hCA II. PMID: 25165709
    13. Myocardial carbonic anhydrase 1/2 activation is significantly elevated in diabetic ischemic cardiomyopathy. PMID: 24670789
    14. may be involved in the pathogenesis of Abdominal aortic aneurysm PMID: 23557951
    15. The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
    16. A significant correlation was found between positive carbonic anhydrase I staining and oral squamous cell carcinoma for more advanced clinical stage and larger tumor size, but not for positive lymph node metastasis, distal metastasis, and recurrence. PMID: 22416960
    17. carbonic anhydrase I, phosphoglycerate kinase 1 and apolipoprotein A-I appeared to be the most significant variations of proteins in patients with osteopenia and osteoarthritis PMID: 22619369
    18. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
    19. Overexpression of CA1 in the synovial tissues of ankylosing spondylitis (AS) patients may promote improper calcification and bone resorption in AS. PMID: 21143847
    20. single mutation of an amino acid not considered essential to catalysis (Phe91Asn) in carbonic anhydrase I, but is near substrate binding pocket, led to increase of catalytic activity; mutant CA I also showed higher affinity for sulfonamide inhibitors PMID: 20624682
    21. The X-ray crystallographic structure of the CA I Michigan 1 variant isozyme is reported, both in the presence and absence of a second bound zinc ion coordinated to His 64, His 200, and Arg 67. PMID: 12009884
    22. concentrations in nondialyzed chronic kidney disease patients, and the relationship with acidosis, zinc, anemia, and iron supplementation PMID: 14675565
    23. cytosolic CA I, II, and XIII are downregulated in neoplastic colorectal mucosa compared to normal colorectal mucosa PMID: 15836783
    24. Isothiocyanato sulfonamide thioureas inhibit this enzyme. PMID: 15837325
    25. X-ray crystallographic structure for the adduct of an activator with hCA I. Binding site interactions of activator L-Histidine with active site amino acids PMID: 16870440
    26. study presents the X-ray structure of the foscarnet adduct with CA1 together with the factors governing recognition of such small molecules by a metalloenzyme active site PMID: 17314045
    27. Decreased levels of carbonic anhydrase 1 isozyme is associated with type II diabetes. PMID: 17464559

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Alpha-carbonic anhydrase family
  • 數據庫鏈接:

    HGNC: 1368

    OMIM: 114800

    KEGG: hsa:759

    STRING: 9606.ENSP00000256119

    UniGene: Hs.23118



主站蜘蛛池模板: 中文字幕无线观看不卡网站| 欧美高清freexxxx性| 97影院在线午夜| 国产精品jk白丝在线播放| 亚洲日韩色欲色欲com| 99热在线精品国产观看| 久久国产精品无码一区二区三区 | 国产一区二区三精品久久久无广告 | 无码av无码天堂资源网影音先锋 | 成 人免费va视频| 国产69精品久久久久乱码免费| 久久和欧洲码一码二码三码| 亚洲综合激情另类专区| 色鬼7777久久| 忘忧草社区中文字幕www| 国产精品久久久久久tv| 秋霞av鲁丝片一区二区| 国产精品免费一区二区三区四区| 亚洲精品国产精品无码国模| 国产亚洲曝欧美曝妖精品| 99久久免费国产精精品| 亚洲鲁丝片一区二区三区| 粗壮挺进人妻水蜜桃成熟漫画| 国产成人精品s8视频| 激情久久av一区av二区av三区| 最近免费中文字幕大全免费版视频| 综合无码精品人妻一区二区三区| 亚洲国产中文在线视频| 国产麻花豆剧传媒精品mv在线| 国产免费看又黄又大又污的胸| 无码精品久久久天天影视| 欧美牲交a欧美牲交aⅴ免费| 午夜无码乱码在线观看| 久久性色欲av免费精品观看| 免费看又色又爽又黄的国产软件| 精品国产乱码久久久久久影片 | 一本色道久久88精品综合| 日日噜噜夜夜狠狠va视频v| 国产欧美二区综合| 奇米影视色777四色在线首页| 777午夜福利理论电影网|