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Human Activity-regulated cytoskeleton-associated protein(ARC) ELISA kit

  • 中文名稱:
    人細(xì)胞骨架活性調(diào)節(jié)蛋白(ARC)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL001981HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人細(xì)胞骨架活性調(diào)節(jié)蛋白(ARC)酶聯(lián)免疫試劑盒(CSB-EL001981HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ARC含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,該產(chǎn)品專為科研場景設(shè)計(jì),可應(yīng)用于細(xì)胞骨架相關(guān)機(jī)制研究,如探究ARC在細(xì)胞遷移、神經(jīng)可塑性或疾病模型(如神經(jīng)退行性疾病、腫瘤轉(zhuǎn)移)中的表達(dá)調(diào)控,也可用于藥物干預(yù)或基因編輯后ARC水平變化的動(dòng)態(tài)監(jiān)測,為體外實(shí)驗(yàn)提供可靠的定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    activity regulated cytoskeleton associated protein ELISA Kit; activity regulated gene 3.1 protein homolog ELISA Kit; Activity-regulated cytoskeleton-associated protein ELISA Kit; Activity-regulated gene 3.1 protein homolog ELISA Kit; Arc ELISA Kit; ARC/ARG3.1 ELISA Kit; ARC_HUMAN ELISA Kit; Arg 3.1 ELISA Kit; Arg3.1 ELISA Kit
  • 縮寫:
    ARC
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 107
    Range % 101-113
    1:2 Average % 105
    Range % 102-109
    1:4 Average % 88
    Range % 85-94
    1:8 Average % 95
    Range % 92-98
  • 回收率:
    The recovery of human ARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-103
    EDTA plasma (n=4) 88 86-92
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.805 2.720 2.763 2.652
    800 1.396 1.515 1.456 1.345
    400 0.786 0.842 0.814 0.703
    200 0.481 0.471 0.476 0.365
    100 0.286 0.275 0.281 0.170
    50 0.182 0.186 0.184 0.073
    25 0.134 0.132 0.133 0.022
    0 0.109 0.112 0.111  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

 常見問題解答
Q:


1) What are the recommended minimum dilutions for human plasma and serum?
2) Is the kit compatible in human CSF or do you have any data indicating that the kit is capable of detecting ARC protein in CSF?
3) Is the kit compatible with samples from non-human primates? Do you have any data from biofluids in NHPs where the kit has had reactivity with NHP ARC protein?

A:
Thanks for your inquiry!
There is no need to dilute the normal human serum and plasma sample generally.
And I will send you manual reference by email later.
As for the CSF and NHPS samples, so sorry that we have no detection because the samples are difficult to collect. Here advice yo to arrange preliminary experiment.
Appreciated to your kind understanding.

靶點(diǎn)詳情

  • 功能:
    Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation.
  • 基因功能參考文獻(xiàn):
    1. The ARC complex revealed association with verbal and total IQ in child. PMID: 28671113
    2. Study identified several rare mutations that reduced ARC expression in schizophrenia patients. Furthermore, DNA methylation of ARC in schizophrenia patients may be associated with a downregulation of ARC mRNA expression. These findings suggest that multiple rare ARC variants and DNA methylation of ARC might contribute to the pathogenesis of schizophrenia. PMID: 27464451
    3. missense mutations in TRIAD3 abolished the interaction of TRIAD3A with Arc, disrupting Arc ubiquitination, and consequently Arc degradation. PMID: 27995769
    4. ARC confers resistance to sunitinib in renal cell carcinoma through alternate angiogenesis pathways PMID: 26995091
    5. We tested whether ARC variants are associated with six measures of cognitive functioning in 670 healthy subjects in the Norwegian Cognitive NeuroGenetics (NCNG) by extracting data from its Genome-Wide Association Study (GWAS). PMID: 26516611
    6. this is the first report associating an ARC SNP with schizophrenia and supports recent large-scale GWAS findings implicating the ARC complex in schizophrenia risk PMID: 26474411
    7. we propose that the peculiar characteristics of Arc protein, such as its optimal expression after ongoing experience or familiar behavior could explain how familiarization and recognition memories are stored and preserved in the mammalian brain--{REVIEW} PMID: 26380114
    8. mRNA levels of ARC and SGK1 did not differ significantly between the schizophrenia or control samples. PMID: 26038830
    9. Arc is a flexible multi-domain protein that exists in monomeric and oligomeric forms, compatible with a diverse, hub-like role in plasticity-related processes. PMID: 25748042
    10. Degradation of Arc by clathrin-localized Triad3A regulates the availability of synaptic AMPA receptors. PMID: 24945773
    11. we report a novel ARC single nucleotide polymorphism associated with a reduced risk of developing Alzheimer disease. PMID: 22622366
    12. analysis of dynamic, multifaceted control of Arc synthesis during mAchR signaling PMID: 22584581
    13. Strong fibrinogen and Abeta deposition is demonstrated in small- and medium-sized vessels, but not in large cerebral arteries, of 24-month-old transgenic arcAbeta mice. PMID: 22302811
    14. Arc Expression Is Increased in medial frontal cortex of patients with Alzheimer's Disease. PMID: 22036569
    15. Arc/MS2 mRNA assembles into particles that move independently, bidirectionally, and intermittently. These observations identify several phases of Arc mRNA movement that serve as potential points for regulating Arc mRNA localization. PMID: 17120280

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  • 亞細(xì)胞定位:
    Extracellular vesicle membrane; Lipid-anchor. Cell junction, synapse, postsynaptic cell membrane; Lipid-anchor. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Early endosome membrane. Cell projection, dendrite. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cell projection, dendritic spine. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族:
    ARC/ARG3.1 family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 648

    OMIM: 612461

    KEGG: hsa:23237

    STRING: 9606.ENSP00000349022

    UniGene: Hs.40888



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