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Human Actin, cytoplasmic 2(ACTG1) ELISA kit

  • 中文名稱:
    人肌動(dòng)蛋白 γ1 (ACTG1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL001222HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人肌動(dòng)蛋白 γ1 (ACTG1)酶聯(lián)免疫試劑盒(CSB-EL001222HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ACTG1含量。試劑盒檢測(cè)范圍為25 pg/mL-1600 pg/mL,為細(xì)胞生物學(xué)研究、疾病模型構(gòu)建及生物標(biāo)志物篩選提供可靠工具;適用于體外培養(yǎng)細(xì)胞功能研究、組織特異性表達(dá)分析及蛋白互作機(jī)制探索等科研場(chǎng)景,助力研究者深入解析細(xì)胞骨架相關(guān)調(diào)控機(jī)制及其在病理生理過程中的作用。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    ACT ELISA Kit; ACTB ELISA Kit; ACTG ELISA Kit; ACTG_HUMAN ELISA Kit; actg1 ELISA Kit; Actin ELISA Kit; Actin; cytoplasmic 2 ELISA Kit; Actin; gamma 1 ELISA Kit; Actin; gamma 1 propeptide ELISA Kit; Actin; gamma ELISA Kit; BRWS2 ELISA Kit; cytoplasmic 2 ELISA Kit; Cytoskeletal gamma actin ELISA Kit; Deafness; autosomal dominant 20 ELISA Kit; Deafness; autosomal dominant 26 ELISA Kit; DFNA20 ELISA Kit; DFNA26 ELISA Kit; epididymis luminal protein 176 ELISA Kit; Gamma-actin ELISA Kit; HEL-176 ELISA Kit; N-terminally processed ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ACTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-99  
    1:2 Average % 105  
    Range % 101-108  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 98  
    Range % 94-102  
  • 回收率:
    The recovery of human ACTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-98  
    EDTA plasma (n=4) 86 80-91  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1600 2.143 2.284 2.214 2.074  
    800 1.441 1.522 1.482 1.342  
    400 0.832 0.807 0.820 0.680  
    200 0.493 0.506 0.500 0.360  
    100 0.301 0.317 0.309 0.169  
    50 0.237 0.245 0.241 0.101  
    25 0.189 0.183 0.186 0.046  
    0 0.139 0.140 0.140    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 基因功能參考文獻(xiàn):
    1. A novel actin gamma 1 (ACTG1) de novo mutation is identified in two sporadic, juvenile, Chinese non-syndromic hearing loss (NSHL) cases using targeted high-throughput sequencing. PMID: 29357087
    2. Results identified ACTG1 with significant high expression in skin cancer tissue and suggested that ACTG1 can regulate the cell proliferation and migration through ROCK signaling pathway. PMID: 28727228
    3. a novel heterozygous missense mutation P32S in the ACTG1 gene was identified in a small family with autosomal dominant nonsyndromic hearing loss. The present findings expand our understanding of the phenotypes associated with ACTG1. Specifically, the results of the present study emphasized that mutations in ACTG1 result in a diverse spectrum of onset ages, including congenital in addition to post-lingual onset. PMID: 29620237
    4. Data reports a recurrent de novo mutation in ACTG1 causes isolated ocular coloboma PMID: 28493397
    5. We have identified a three-generation pedigree segregating a novel mutation in the ACTG1 gene that causes Baraitser-Winter Syndrome with extremely variable expressivity, leading to an initial diagnosis of isolated AD hearing loss in two members. PMID: 27096712
    6. we expand the clinical spectrum of the ACTG1-associated Baraitser-Winter cerebrofrontofacial syndrome demonstrating the mild end of the facial and brain manifestations. PMID: 27240540
    7. Data indicate that F-actin is significantly elevated in septic shock, and F-actin and the F:G-actin ratio are potential biomarkers for the diagnosis of septic shock. PMID: 26754286
    8. a novel mutation in ACTG1 was found to be co-segregated with hearing loss and the genetic cause of autosomal dominant nonsyndromic hearing impairment in a Chinese family PMID: 26832775
    9. Three unrelated cases of rare ACTG1 variants in fetal microlissencephaly have been described. PMID: 26188271
    10. In this study we sought to clarify the detailed mutational spectrum, clinical features, and genotype-phenotype correlations for ACTG1 gene associated autosomal dominant sensorineural hearing loss PMID: 25792668
    11. The data, for the first time, link ASAP3 with ACTG1 in the regulation of cytoskeletal maintenance and cell motility PMID: 24284654
    12. The actin/MKL1 signalling pathway influences cell growth and gene expression through large-scale chromatin reorganization and histone post-translational modifications. PMID: 24762104
    13. the novel variant p.M305T in ACTG1 (DFNA20/26) was selected as a disease-causing variant. PMID: 23506231
    14. These results showed the biphasic F-actin dynamics in herpes simplex virus 1 neuronal infection and confirmed the association of F-actin with the changes in the expression and activity of cofilin 1. PMID: 22623803
    15. knockdown of gamma-actin significantly reduced speed of motility and severely affected the cell's ability to explore, which was, in part, due to a loss of cell polarity PMID: 21908715
    16. Cytoplasmic G-actin concentration is a critical parameter for determining the extent of stimulus-induced G-actin assembly and cell extension. PMID: 21502360
    17. actin participates in transcription elongation by recruiting Cdk9,a catalytic subunit of P-TEFb, for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling PMID: 21378166
    18. RNA polymerase II accumulates in the promoter-proximal region of the dihydrofolate reductase and gamma-actin genes. PMID: 12612070
    19. the first description of a mutation in cytoskeletal, or nonmuscle, actin; with an autosomal dominant, progressive, sensorineural hearing loss phenotype PMID: 13680526
    20. A missense mutation in the gamma actin 1 gene causes autosomal dominant hearing loss. PMID: 14684684
    21. Our data are consistent with the idea that DP assembles into nascent junctions from both diffusible and particulate pools in a temporally overlapping series of events triggered by cell-cell contact and regulated by actin and DP-IF interactions. PMID: 16365169
    22. Espins do not activate the Arp2/3 complex in vitro, and bundle assembly is not indicative of in-vitro nucleation activity.Our results suggest a novel way to build actin bundles at specific sites in cells. PMID: 16569662
    23. A major factor in the deafness caused by gamma-actin mutations is an altered ability of the actin filaments to be properly regulated by actin-binding proteins rather than an inability to polymerize. PMID: 16690605
    24. the present results do not indicate that mutations in ACTG1 are a frequent cause of autosomal-dominant postlingual sensorineural hearing impairment in Norway nor Denmark. PMID: 16773128
    25. actin and vimentin filaments can interact directly through the tail domain of vimentin PMID: 16901892
    26. During cell membrane blebbing actin recruitment is involved in cortex assembly. PMID: 17088428
    27. In both AA and AL renal amyloidosis groups there were significant positive correlations between immunostaining of TGFbeta-1 and alpha-SMA. PMID: 17285762
    28. IQGAP1 regulates Salmonella invasion through interactions with actin, Rac1, and Cdc42 PMID: 17693642
    29. In this report we have used mutagenesis of the Talin1 I/LWEQ module to show that the amino acids responsible for dimerization are necessary for F-actin binding, stabilization and cross-linking of actin filaments, and focal adhesion targeting. PMID: 17722883
    30. analysis of the structural basis of profilin-actin complexes during filament elongation by Ena/VASP PMID: 17914456
    31. In this study, a novel missense mutation (c.364A>G; p.I122V) co-segregated with the affected individuals in the family and did not exist in the unaffected family members and 150 unrelated normal controls. PMID: 18804074
    32. Both RPEL peptides of the MAL protein bind to the G-actin hydrophobic cleft and to subdomain 3. PMID: 19008859
    33. Two novel ACTG1 missense mutations are associated with DFNA20/26 hearing impairment. PMID: 19477959
    34. audiometric phenotype of the Dutch DFNA20/26 family with a novel mutation in ACTG1 was largely consistent with previous reports on DFNA20/26. All suffered from hearing loss.This is the first known DFNA20/26 family that has experienced tinnitus. PMID: 19548389

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  • 相關(guān)疾病:
    Deafness, autosomal dominant, 20 (DFNA20); Baraitser-Winter syndrome 2 (BRWS2)
  • 亞細(xì)胞定位:
    Cytoplasm, cytoskeleton.
  • 蛋白家族:
    Actin family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 144

    OMIM: 102560

    KEGG: hsa:71

    STRING: 9606.ENSP00000331514

    UniGene: Hs.514581



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