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Fish transforming growth factor, beta 1 (TGFB1) ELISA kit

說明書下載

產品詳情

產品描述：

魚轉化生長因子β1(TGFB1)酶聯免疫試劑盒（CSB-EL023446FI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TGFB1含量。TGFB1 即轉化生長因子 -β1，在細胞生長、分化、凋亡等多種生物學進程中發揮關鍵作用。其異常表達與腫瘤、纖維化等多種疾病密切相關。研究機制主要聚焦于其信號通路，通過調節下游基因表達影響細胞行為，為相關疾病治療提供潛在靶點。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研領域探究環境脅迫、病原感染或營養代謝對魚類免疫系統的影響，也可用于水產養殖中魚類健康狀態評估及免疫相關分子通路研究，為水生動物免疫學、病理學及生態毒理學研究提供可靠的分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TGFB1
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	92
	Range %	85-105
1:4	Average %	97
	Range %	90-102
1:8	Average %	95
	Range %	90-105

回收率：

The recovery of fish TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	94	85-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.