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Chicken secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名稱:
    雞分泌型免疫球蛋白A(SIgA)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E10097Ch
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    雞分泌型免疫球蛋白A(SIgA)酶聯(lián)免疫試劑盒(CSB-E10097Ch)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的SIgA含量。SIgA即分泌型免疫球蛋白 A,是黏膜免疫的關(guān)鍵效應(yīng)分子。它可阻止病原體附著、中和毒素,在呼吸道、胃腸道等黏膜表面發(fā)揮重要保護(hù)作用。研究聚焦其產(chǎn)生機(jī)制、與疾病關(guān)聯(lián)等,以探尋基于 SIgA 的疾病防治手段。試劑盒檢測(cè)范圍為0.234 μg/mL-60 μg/mL,可滿足實(shí)驗(yàn)動(dòng)物模型、疫苗開發(fā)等研究中黏膜免疫應(yīng)答的動(dòng)態(tài)監(jiān)測(cè)需求,例如評(píng)估腸道病原感染后的免疫狀態(tài)、飼料添加劑對(duì)黏膜免疫的影響或疫苗免疫效果分析;為禽類免疫學(xué)、獸醫(yī)學(xué)及比較醫(yī)學(xué)領(lǐng)域的科研工作者提供可靠的檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    SIgA
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    0.234 μg/mL-60 μg/mL
  • 靈敏度:
    0.058 μg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 86  
    Range % 82-89  
    1:400 Average % 98  
    Range % 92-104  
    1:800 Average % 97  
    Range % 93-101  
    1:1600 Average % 88  
    Range % 84-92  
  • 回收率:
    The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 104 98-108  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    60 0.345 0.346 0.346    
    15 0.565 0.593 0.579    
    3.75 0.856 0.872 0.864    
    0.94 1.158 1.197 1.178    
    0.234 1.367 1.289 1.328    
    0 1.881 1.825 1.853    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

 常見問題解答
Q:

For the kit CSB-E10097Ch,may I know if it can be tested with tissue samples, specifically small intestine?
Would you have this information available to share?

A:
Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.


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