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USP7

USP7,即泛素特異性肽酶7,別名TEF1、HAUSP、HAFOUS,是一種去泛素化酶,屬于肽酶C19家族,其中包括泛素水解酶。
USP7可通過去泛素化目標(biāo)蛋白質(zhì),如p53(一種腫瘤抑制蛋白)和WASH(內(nèi)體蛋白質(zhì)回收所必需的),調(diào)節(jié)它們的活動,這通過抵消參與相應(yīng)過程的蛋白質(zhì)(如HDM2和TRIM27)的相反泛素連接酶活性來實現(xiàn)。
USP7在細(xì)胞周期、DNA修復(fù)、染色質(zhì)重塑和表觀遺傳調(diào)控中扮演關(guān)鍵角色。異常激活或過表達(dá)的USP7可能促進(jìn)腫瘤發(fā)生和病毒性疾病,使其成為治療干預(yù)的靶點。USP7還與多種疾病相關(guān),包括神經(jīng)發(fā)育障礙等。

熱銷產(chǎn)品

Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7),Partial (CSB-EP849973HU)

驗證數(shù)據(jù)

CSB-EP849973HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

USP7 Recombinant Monoclonal Antibody (CSB-RA192026A0HU)

驗證數(shù)據(jù)

CSB-RA192026A0HU

Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: HAUSP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 129, 127 kDa
Observed band size: 140 kDa

CSB-RA192026A0HU

IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

CSB-RA192026A0HU

IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

CSB-RA192026A0HU

Immunofluorescence staining of Hela Cells with CSB-RA192026A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

USP7 Antibodies

USP7 for Homo sapiens (Human)

USP7 Proteins

USP7 Proteins for Rattus norvegicus (Rat)

USP7 Proteins for Mus musculus (Mouse)

USP7 Proteins for Gallus gallus (Chicken)

USP7 Proteins for Homo sapiens (Human)

USP7 Proteins for Drosophila melanogaster (Fruit fly)



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