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TSPO

TSPO(轉運蛋白)全稱為線粒體外膜轉位蛋白,也被稱為外周型苯二氮?受體或18 kDa線粒體膜蛋白。TSPO廣泛分布于神經、免疫及內分泌系統中,主要參與膽固醇跨線粒體膜運輸,調控類固醇激素合成,并與線粒體功能、細胞凋亡密切相關。TSPO表達異常與阿爾茨海默病、帕金森病等神經退行性疾病相關,在腦損傷區域或腫瘤組織中常出現高表達現象。近年來TSPO作為生物標志物被用于神經炎癥和膠質瘤的PET影像診斷,其特異性配體PK11195已在臨床中應用。針對焦慮癥和創傷后應激障礙,以TSPO為靶點的藥物如埃替拉侖已進入II期臨床試驗,顯示出調節神經類固醇合成的潛力。在癌癥領域,靶向TSPO的藥物可通過誘導腫瘤細胞線粒體膜電位崩潰促進凋亡,目前多項基于TSPO調控的抗癌方案正在臨床前驗證階段。

熱銷產品

TSPO Recombinant Monoclonal Antibody (CSB-RA025168A0HU)

驗證數據

CSB-RA025168A0HU

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: PBR antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19, 11 KDa
Observed band size: 19 KDa

CSB-RA025168A0HU

IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-RA025168A0HU

IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-RA025168A0HU

Immunofluorescence staining of PC3 cells with CSB-RA025168A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CSB-RA025168A0HU

Immunoprecipitating PTGS2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA025168A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA025168A0HU (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

CSB-RA025168A0HU

Overlay histogram showing HepG2 cells stained with CSB-RA025168A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

TSPO Antibodies

TSPO for Homo sapiens (Human)

TSPO Proteins

TSPO Proteins for Rattus norvegicus (Rat)

TSPO Proteins for Bos taurus (Bovine)

TSPO Proteins for Homo sapiens (Human)

TSPO Proteins for Mus musculus (Mouse)

TSPO Proteins for Arabidopsis thaliana (Mouse-ear cress)

TSPO Proteins for Sus scrofa (Pig)

TSPO Proteins for Ovis aries (Sheep)



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