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Your Good Partner in Biology Research

TOP1

TOP1作為拓撲異構酶,在DNA復制和轉錄過程中扮演著至關重要的角色。其獨特的酶結構使其能夠與DNA緊密結合,并通過催化DNA解旋和重連來解決復制和轉錄過程中產生的拓撲問題。TOP1的主要功能是確保DNA雙螺旋在復制和轉錄時的順暢進行,防止因拓撲約束而阻礙進程。在信號通路上,TOP1與其他復制和轉錄因子相互協作,響應DNA復制和轉錄的調控機制,共同維護基因組的穩定性和完整性。深入研究TOP1的結構、功能和信號通路,對于理解細胞生物學和基因組學的復雜機制具有重要意義,也為開發新的治療策略和藥物靶點提供了潛在的方向。

熱銷產品

TOP1 Recombinant Monoclonal Antibody

驗證數據

WB
CSB-RA792129A0HU WB

Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate
All lanes: TOP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa

FITC
CSB-RA792129A0HU FITC

Overlay histogram showing HepG2 cells stained with CSB-RA792129A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

IP
CSB-RA792129A0HU IP

Immunoprecipitating TOP1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA792129A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA792129A0HU(2μg)+ K562 whole cell lysate(500μg)
Lane 3: K562 whole cell lysate (10μg)

IHC
CSB-RA792129A0HU IHC-1

IHC image of CSB-RA792129A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC
CSB-RA792129A0HU IHC-2

IHC image of CSB-RA792129A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


TOP1 Antibodies

TOP1 for Homo sapiens (Human)

TOP1 Proteins

TOP1 Proteins for Homo sapiens (Human)



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