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ROBO1

ROBO1全稱為Roundabout同源受體1,也被稱為DUTT2或SAX3,屬于軸突導(dǎo)向受體家族。ROBO1蛋白通過(guò)結(jié)合Slit配體調(diào)控神經(jīng)細(xì)胞遷移和軸突導(dǎo)向,在胚胎發(fā)育中參與神經(jīng)網(wǎng)絡(luò)構(gòu)建,在腫瘤血管生成和癌細(xì)胞轉(zhuǎn)移中同樣發(fā)揮重要作用。ROBO1/Slit信號(hào)異常與肺癌、乳腺癌和結(jié)直腸癌等惡性腫瘤的侵襲性增強(qiáng)相關(guān),其過(guò)表達(dá)可能通過(guò)激活MAPK和PI3K-AKT通路促進(jìn)腫瘤細(xì)胞存活與遷移。ROBO1基因缺失或突變還與先天性心臟病、智力發(fā)育障礙存在關(guān)聯(lián)。目前針對(duì)ROBO1的藥物開(kāi)發(fā)聚焦于抗腫瘤領(lǐng)域,已有研究團(tuán)隊(duì)開(kāi)發(fā)出特異性單克隆抗體,在動(dòng)物模型中顯示出抑制腫瘤轉(zhuǎn)移的效果。ROBO1的雙重作用機(jī)制使其在神經(jīng)修復(fù)和抗腫瘤領(lǐng)域都具有潛在開(kāi)發(fā)價(jià)值。

熱銷產(chǎn)品

ROBO1 Recombinant Monoclonal Antibody (CSB-RA896760MA1HU)

驗(yàn)證數(shù)據(jù)

CSB-RA896760MA1HU

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA896760MA1HU

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA896760MA1HU

Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-RA896760MA1HU

Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-RA896760MA1HU

Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,025 events was performed.

Human Roundabout homolog 1(ROBO1) ELISA kit (CSB-EL020054HU)

驗(yàn)證數(shù)據(jù)

CSB-EL020054HU 標(biāo)準(zhǔn)曲線

貨號(hào):CSB-EL020054HU

規(guī)格:96T/48T

靈敏度:0.039 ng/mL

檢測(cè)范圍:0.156 ng/mL-10 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ROBO1 Antibodies

ROBO1 for Homo sapiens (Human)

ROBO1 Proteins

ROBO1 Proteins for Homo sapiens (Human)

ROBO1 ELISA Kit

ROBO1 ELISA Kit for Homo sapiens (Human)



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