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PODXL

PODXL,全名為Podocalyxin-like,常見別名為CD34相關蛋白。該蛋白主要表達于血管內皮細胞,具有調節細胞粘附和遷移的功能。PODXL通過影響細胞表面電荷分布,參與血管的穩定性和通透性調節。在生理狀態下,它有助于維持正常的血管結構和功能。然而,當PODXL表達異常時,可能與多種疾病的發生發展相關,如癌癥轉移、動脈粥樣硬化等。近年來,針對PODXL的藥物研發取得了一定進展,例如通過抑制PODXL的表達或活性,來阻止腫瘤細胞的侵襲和轉移。此外,一些抗血管生成藥物也顯示出與PODXL相互作用的潛力,為相關疾病的治療提供了新的思路。

熱銷產品

Recombinant Human Podocalyxin (PODXL), partial (CSB-YP518830HU)

驗證數據

CSB-YP518830HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-YP518830HU

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.

CSB-YP518830HU

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.

PODXL Monoclonal Antibody (CSB-MA134471A0m)

驗證數據

CSB-MA134471A0m

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

CSB-MA134471A0m

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

CSB-MA134471A0m

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

CSB-MA134471A0m

Western Blot
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
All lanes: PODXL antibody at 1.28µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

CSB-MA134471A0m

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

CSB-MA134471A0m

Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

CSB-MA134471A0m

Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA134471A0m

Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA134471A0m

Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA134471A0m

Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA134471A0m

Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA134471A0m

Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)

PODXL Antibodies

PODXL for Homo sapiens (Human)

PODXL Proteins

PODXL Proteins for Homo sapiens (Human)

PODXL Proteins for Gallus gallus (Chicken)

PODXL Proteins for Oryctolagus cuniculus (Rabbit)

PODXL Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

PODXL Proteins for Mus musculus (Mouse)

PODXL Proteins for Rattus norvegicus (Rat)

PODXL ELISA Kit

PODXL ELISA Kit for Homo sapiens (Human)

PODXL ELISA Kit for Rattus norvegicus (Rat)



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