(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized MSLN at 2 μg/ml can bind Anti-MSLN rabbit monoclonal antibody, the EC₅₀ of the MSLN protein is 2.657-3.177 ng/ml.

Activity
Measured by its binding ability in a functional ELISA. Immobilized MUC16(CSB-MP704410HU3c7) at 10 μg/ml can bind MSLN, the EC₅₀ is 460.7-662.2 ng/ml.

Western blot
All lanes: MSLN antibody at 8µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 69, 72, 68 kDa
Observed band size: 69 kDa

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA015044ESR1HU at dilution of 1:100