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LAT

LAT,全名為“激活T細胞的接頭蛋白”,也被稱為LAT1、pp36、IMD52。該蛋白在T細胞抗原受體(TCR)信號轉導通路中發揮關鍵作用,通過磷酸化后,將多個銜接蛋白和下游信號分子募集到信號復合物中,從而激活T細胞。LAT蛋白與多種疾病相關,如免疫缺陷癥等。在藥物研發方面,針對LAT蛋白的藥物正在研發中,旨在治療相關疾病。

熱銷產品

Phospho-LAT (Y191) Recombinant Monoclonal Antibody (CSB-RA012767A191phHU)

驗證數據

CSB-RA012767A191phHU

Western Blot
Positive WB detected in:Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate(treated with EGF or Pervanadate)
All lanes: Phospho-LAT antibody at 2.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 KDa
Observed band size: 38 KDa

CSB-RA012767A191phHU

IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-RA012767A191phHU

IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-RA012767A191phHU

Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CSB-RA012767A191phHU

Immunoprecipitating Phospho-LAT in A549 whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of CSB-RA012767A191phHU in A549 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA012767A191phHU(3μg)+ A549 whole cell lysate(1mg)
Lane 3: A549 whole cell lysate (20μg)

LAT Antibodies

LAT for Homo sapiens (Human)

LAT Proteins

LAT Proteins for Mus musculus (Mouse)

LAT Proteins for Rattus norvegicus (Rat)

LAT Proteins for Homo sapiens (Human)

LAT Proteins for Mycobacterium bovis



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