(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Immunofluorescence?staining?of?Hela?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L).

Immunofluorescence?staining?of U251?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L).

Overlay Peak curve showing Jurkat cells stained with CSB-RA751251MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100.Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.