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ERBB2

ERBB2,即人表皮生長因子受體2,別名NEU、HER-2、CD340、HER2,由人類ERBB2基因編碼,該基因位于7號染色體上。ERBB2蛋白是一種跨膜酪氨酸激酶受體,屬于HER家族成員之一,由胞外配體結合域、跨膜結構域和胞內結構域三部分組成。其作用機制涉及與其他HER家族成員結合形成異二聚體,增強激酶的激活作用并介導下游信號通路的活性,如MAPK和PI3K/AKT信號通路。
ERBB2的異常激活,包括基因擴增、過表達和突變,與多種癌癥的發(fā)生發(fā)展有關,如乳腺癌、非小細胞肺癌、胃癌和子宮內膜腫瘤等。ERBB2基因擴增與乳腺癌的預后不良相關,已成為近30%乳腺癌患者生物治療的重要靶點。

熱銷產品

Recombinant Human Epidermal growth factor receptor (EGFR), partial (Active) (CSB-MP007479HU)

驗證數據

CSB-MP007479HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-MP007479HU

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.

CSB-MP007479HU

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.

CSB-MP007479HU

Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.

CSB-MP007479HU

Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.

CSB-MP007479HU

Activity
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.

CSB-MP007479HU

The purity of EGFR was greater than 95% as determined by SEC-HPLC

EGFR Recombinant Monoclonal Antibody (CSB-RA159341MA1HU)

驗證數據

CSB-RA159341MA1HU

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA159341MA1HU

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA159341MA1HU

Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ERBB2 Antibodies

ERBB2 for Homo sapiens (Human)

ERBB2 for H, M, R

ERBB2 for H

ERBB2 Proteins

ERBB2 Proteins for Mus musculus (Mouse)

ERBB2 Proteins for Rattus norvegicus (Rat)

ERBB2 Proteins for Homo sapiens (Human)

ERBB2 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

ERBB2 Proteins for Mesocricetus auratus (Golden hamster)

ERBB2 ELISA Kit

ERBB2 ELISA Kit for Homo sapiens (Human)



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