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Your Good Partner in Biology Research

CHEK1

CHEK1,別名CHK1、OZEMA21,是一種由人類基因編碼的Ser/Thr蛋白激酶家族成員,對響應DNA損傷或未復制DNA存在的檢查點調節細胞周期停滯至關重要。CHEK1可整合來自ATM和ATR兩種參與DNA損傷應答的細胞周期蛋白的信號,這些蛋白在減數分裂前期I中也與染色質相關。CHEK1對CDC25A蛋白磷酸酶的磷酸化是必需的,以使細胞響應雙鏈DNA斷裂而延遲細胞周期進程。CHEK1參與DNA損傷檢查點信號傳導、G2/M轉換的有絲分裂細胞周期以及內細胞團細胞增殖等過程。它在染色質、核和復制叉中發揮作用,并在多種組織中表達,包括腎上腺髓質、早孕、性腺、直覺和來自神經嵴的軀干間充質。CHEK1的異常表達與多種癌癥相關,包括胰腺癌。

熱銷產品

CHEK1 Recombinant Monoclonal Antibody (CSB-RA176809A0HU)

驗證數據

CSB-RA176809A0HU

Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
All lanes: Chk1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44, 51 kDa
Observed band size: 55 kDa

CSB-RA176809A0HU

IHC image of CSB-RA176809A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

CSB-RA176809A0HU

Immunofluorescence staining of Hela Cells with CSB-RA176809A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CHEK1 Antibodies

CHEK1 for Homo sapiens (Human)

CHEK1 Proteins

CHEK1 Proteins for Homo sapiens (Human)

CHEK1 Proteins for Mus musculus (Mouse)

CHEK1 Proteins for Xenopus laevis (African clawed frog)

CHEK1 Proteins for Gallus gallus (Chicken)

CHEK1 Proteins for Rattus norvegicus (Rat)



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