CEACAM5
CEACAM5,即癌胚抗原細(xì)胞黏附分子5,別名CD66e,由人類CEACAM5基因編碼,該基因位于染色體19q13.2,基因長度約為21kb,由9個外顯子和3個非編碼外顯子組成。CEACAM5通過糖基磷脂酰肌醇(GPI)與膜結(jié)合,胞外共有7個免疫球蛋白樣結(jié)構(gòu)域,包括一個可變(IgV)樣結(jié)構(gòu)域和6個IgC樣結(jié)構(gòu)域(A1-B3)。
CEACAM5在多種生物學(xué)過程中發(fā)揮作用,包括細(xì)胞黏附、細(xì)胞信號轉(zhuǎn)導(dǎo)和免疫調(diào)節(jié)等,并在許多惡性腫瘤細(xì)胞中高表達(dá),被廣泛用于癌癥的診斷和監(jiān)測。CEACAM5的表達(dá)異常與多種癌癥的發(fā)生發(fā)展相關(guān),尤其在結(jié)直腸癌、胃癌、肺癌和胰腺癌中過表達(dá),是潛在的癌癥治療靶點(diǎn)。
熱銷產(chǎn)品
驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA1HU), the EC50 is 0.8955-1.719 ng/mL.
驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CEACAM5 at 2μg/ml can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA4HU). The EC50 is 3.572-4.044 ng/mL.

The purity of CEACAM5 was greater than 95% as determined by SEC-HPLC
CEACAM5 Recombinant Monoclonal Antibody (CSB-RA005165MA3HU)
驗(yàn)證數(shù)據(jù)

Immunofluorescence staining of Hela cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).

Immunofluorescence staining of MCF7 cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA005165MA3HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was human IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.
CEACAM5 Antibodies
CEACAM5 for H
產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-MA000218 | CEACAM5 Monoclonal Antibody | Human | ELISA,IHC |
CEACAM5 for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-PA005033 | CEACAM5 Antibody | Human | WB, ELISA |
CSB-PA160116 | CEACAM5 Antibody | Human | ELISA,WB,IHC |
CSB-PA229032 | CEACAM5 Antibody | Human | ELISA,WB,IHC |
CSB-PA109108 | CEACAM5 Antibody | Human | ELISA,IHC |
CSB-PA097333 | CEACAM5 Antibody | Human | ELISA,IHC |
CSB-PA005165LA01HU | CEACAM5 Antibody | Human | ELISA, WB, IHC, IF |
CSB-PA005165LB01HU | CEACAM5 Antibody, HRP conjugated | Human | ELISA |
CSB-PA005165LC01HU | CEACAM5 Antibody, FITC conjugated | Human | |
CSB-PA005165LD01HU | CEACAM5 Antibody, Biotin conjugated | Human | ELISA |
CSB-MA995583 | CEACAM5 Monoclonal Antibody | Human | ELISA, IHC |
CSB-RA005165MA3HU | CEACAM5 Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
CSB-RA005165MA1HU | CEACAM5 (labetuzumab) Recombinant Monoclonal Antibody | Human | ELISA |
CEACAM5 Proteins
CEACAM5 Proteins for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP005165HU | Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K) | Yeast |
CSB-MP005165HU | Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K) (Active) | Mammalian cell |
CEACAM5 Proteins for Mus musculus (Mouse)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP005165MO CSB-EP005165MO CSB-BP005165MO CSB-EP005165MO-B |
Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CEACAM5 ELISA Kit
CEACAM5 ELISA Kit for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
---|---|---|---|
CSB-E04767h | Human carcinoembryonic antigen,CEA ELISA Kit | serum, plasma, tissue homogenates | 2 ng/mL |
CEACAM5 ELISA Kit for Mus musculus (Mouse)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
---|---|---|---|
CSB-E13925m | Mouse carcinoembryonic antign,CEA ELISA Kit | serum, plasma, tissue homogenates | 15.6 pg/mL |