在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

CDK6

CDK6,即細胞周期蛋白依賴性激酶6,別名細胞分裂蛋白6、血小板來源的絲氨酸/蘇氨酸激酶等。它是一種由CDK6基因編碼的蛋白激酶,屬于CMGC蛋白激酶家族成員,對細胞周期G1期的進展和G1/S轉換具有重要作用。CDK6與D型細胞周期蛋白結合,形成復合體,通過磷酸化視網膜母細胞瘤蛋白(Rb),激活E2F轉錄程序,促進細胞進入S期進行DNA復制。CDK6在生物學上的意義主要體現在其對細胞周期的調控作用。它的活性對于維持細胞的正常增殖至關重要,同時CDK6也參與了細胞分化和腫瘤細胞的增殖。在腫瘤細胞中,CDK6的異常激活與多種癌癥的發生發展有關,包括白血病、淋巴瘤和實體瘤等。此外,CDK6抑制劑如Palbociclib、Ribociclib和Abemaciclib已在臨床上用于治療某些類型的乳腺癌,并正在被研究用于治療其他癌癥類型。

熱銷產品

CDK6 Recombinant Monoclonal Antibody (CSB-RA555745A0HU)

驗證數據

CSB-RA555745A0HU

Western Blot
Positive WB detected in: JK whole cell lysate(20μg), K562 whole cell lysate(20μg), SY5Y whole cell lysate(20μg), HepG2 whole cell lysate(20μg), COLO205 whole cell lysate(20μg)
All lanes: CDK6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 120s

CSB-RA555745A0HU

Immunofluorescence staining of U251 cell with CSB-RA555745A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-RA555745A0HU

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-RA555745A0HU

Immunofluorescence staining of Hela cell with CSB-RA555745A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-RA555745A0HU

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-RA555745A0HU

IHC image of CSB-RA555745A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

CSB-RA555745A0HU

IHC image of CSB-RA555745A0HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

CSB-RA555745A0HU

Immunoprecipitating CDK6 in K562whole cell lysate
Lane 1: CSB-RA555745A0HU(3μg)+ K562 whole cell lysate(220μg)
Lane 2: K562 whole cell lysate(30μg)
Lane 3:Rabbit control IgG instead of CSB-RA010605A0HU?in?K562?whole?cell?lysate
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/40000)

CDK6 Antibodies

CDK6 for Homo sapiens (Human)

CDK6 Proteins

CDK6 Proteins for Mus musculus (Mouse)