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CDH1

CDH1,即鈣粘蛋白1,別名E-cadherin、CD324、uvomorulin、ECAD和CDHE。它是一種鈣依賴性的細胞粘附分子,由人類基因組編碼,在上皮細胞中高度表達。CDH1的作用機制主要通過其跨膜結構域和細胞外鈣粘蛋白重復序列,介導細胞間的同型粘附。這種粘附對于維持上皮組織的完整性和形態至關重要。CDH1在組織發育、細胞遷移、增殖以及上皮屏障功能中發揮重要作用。CDH1的表達失調與多種疾病相關,尤其是癌癥的發生、發展、侵襲和轉移。CDH1的缺失或功能喪失會導致細胞間粘附減弱,增加細胞的運動性,從而促進腫瘤細胞的侵襲和轉移。CDH1被視為一種腫瘤抑制因子,其在多種癌癥治療和預后評估中具有潛在的應用價值。

熱銷產品

Recombinant Human Cadherin-1(CDH1),partial (Active) (CSB-MP005034HU1)

驗證數據

CSB-MP005034HU1

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-MP005034HU1

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 2μg/mL can bind Anti-CDH1 recombinant antibody(CSB-RA005034MA2HU),the EC50 is 5.151-6.358 ng/mL.

CDH1 Recombinant Monoclonal Antibody (CSB-RA005034MA1HU)

驗證數據

CSB-RA005034MA1HU

Western Blot
Positive WB detected in: Mouse Liver tissue lysate
All lanes: CDH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 80-135 kDa
Observed band size: 135 kDa

CSB-RA005034MA1HU

IHC image of CSB-RA005034MA1HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA005034MA1HU

Overlay Peak curve showing MCF7 cells stained with CSB-RA005034MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.

CDH1 Antibodies

CDH1 for Homo sapiens (Human)

CDH1 Proteins

CDH1 Proteins for Homo sapiens (Human)

CDH1 Proteins for Gallus gallus (Chicken)

CDH1 Proteins for Mus musculus (Mouse)

CDH1 Proteins for Xenopus laevis (African clawed frog)

CDH1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

CDH1 Proteins for Dictyostelium discoideum (Slime mold)

CDH1 Proteins for Pongo abelii (Sumatran orangutan)

CDH1 Proteins for Bos taurus (Bovine)

CDH1 Proteins for Rattus norvegicus (Rat)

CDH1 ELISA Kit

CDH1 ELISA Kit for Homo sapiens (Human)

CDH1 ELISA Kit for Mus musculus (Mouse)

CDH1 ELISA Kit for Rattus norvegicus (Rat)



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