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CD40

CD40,即腫瘤壞死因子受體超家族成員5(TNFRSF5),別名Bp50、CD40L等。CD40由人類CD40基因編碼,廣泛表達于免疫細胞,特別是B細胞、樹突狀細胞(DC)和單核細胞。CD40是一種單鏈型跨膜蛋白,包括一個胞外區域、一個跨膜區域和一個胞漿區域,其胞外區域可以與其配體CD40L(CD40配體)結合,觸發信號傳導。CD40在連接先天免疫和適應性免疫方面發揮著重要作用,其功能擴展到B細胞激活、抗原呈遞和炎癥調節等多個層面。CD40對于體液免疫應答、免疫記憶的形成以及免疫調節都具有重要意義。CD40信號傳導也在免疫治療研究中被廣泛研究,以開發新的治療方法來增強或抑制免疫系統的特定響應。

熱銷產品

Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) (CSB-MP004936HU1)

驗證數據

CSB-MP004936HU1

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-MP004936HU1

Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/ml can bind CD40L (CSB-MP004937HU3), the EC50 is 3.112-3.858 ng/ml.

CSB-MP004936HU1

Activity
Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.

CSB-MP004936HU1

The purity of CD40 was greater than 95% as determined by SEC-HPLC

CD40 Recombinant Monoclonal Antibody (CSB-RA004936MA1HU)

驗證數據

CSB-RA004936MA1HU

Immunofluorescence staining of A549 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

CSB-RA004936MA1HU

Immunofluorescence staining of PC-3 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

CSB-RA004936MA1HU

Overlay Peak curve showing THP-1 cells surface stained with CSB-RA004936MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,012 events was performed.

CD40 Antibodies

CD40 for Homo sapiens (Human)

CD40 Proteins

CD40 Proteins for Mus musculus (Mouse)

CD40 Proteins for Homo sapiens (Human)

CD40 Proteins for Bos taurus (Bovine)

CD40 Proteins for Callithrix jacchus (White-tufted-ear marmoset)

CD40 Proteins for Equus caballus (Horse)

CD40 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

CD40 Proteins for Sus scrofa (Pig)

CD40 ELISA Kit

CD40 ELISA Kit for Mus musculus (Mouse)