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AKT1

AKT1,即蛋白激酶Bα,是一種在細(xì)胞內(nèi)信號傳導(dǎo)中發(fā)揮關(guān)鍵作用的絲氨酸/蘇氨酸激酶。AKT1廣泛存在于多種組織和細(xì)胞類型中,是PI3K/AKT信號通路的核心組分。其作用機(jī)制是通過PI3K(磷脂酰肌醇3激酶)產(chǎn)生的磷脂酰肌醇-3,4,5-三磷酸(PIP3)的招募,進(jìn)而被激活。激活后的AKT1能夠磷酸化多種底物蛋白,調(diào)控細(xì)胞的生長、存活、代謝和凋亡等過程。AKT1參與的信號通路包括PI3K/AKT/mTOR通路,這一通路在細(xì)胞增殖、蛋白質(zhì)合成、細(xì)胞代謝和細(xì)胞存活中起著至關(guān)重要的作用。AKT1還與細(xì)胞周期調(diào)控、細(xì)胞應(yīng)激反應(yīng)和細(xì)胞遷移等過程相關(guān)聯(lián)。在生物學(xué)意義上,AKT1對于維持細(xì)胞內(nèi)環(huán)境的穩(wěn)定和適應(yīng)外界變化至關(guān)重要。其功能異常與多種疾病有關(guān),尤其是與腫瘤的發(fā)生發(fā)展密切相關(guān),是癌癥治療中的一個潛在靶點。

熱銷產(chǎn)品

Recombinant Rat RAC-alpha serine/threonine-protein kinase (Akt1) (CSB-EP001553RA)

驗證數(shù)據(jù)

CSB-EP001553RA

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

AKT1 Recombinant Monoclonal Antibody (CSB-RA917625A0HU)

驗證數(shù)據(jù)

CSB-RA917625A0HU

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue, Rat heart tissue
All lanes: AKT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

CSB-RA917625A0HU

IHC image of CSB-RA917625A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

CSB-RA917625A0HU

Immunofluorescence staining of Hela Cells with CSB-RA917625A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

AKT1 Antibodies

AKT1 for Homo sapiens (Human)

AKT1 Proteins

AKT1 Proteins for Homo sapiens (Human)

AKT1 Proteins for Mus musculus (Mouse)

AKT1 Proteins for Rattus norvegicus (Rat)

AKT1 Proteins for Oryza sativa subsp. indica (Rice)

AKT1 Proteins for Oryza sativa subsp. japonica (Rice)

AKT1 Proteins for Arabidopsis thaliana (Mouse-ear cress)

AKT1 Proteins for Drosophila melanogaster (Fruit fly)

AKT1 Proteins for Xenopus laevis (African clawed frog)

AKT1 ELISA Kit

AKT1 ELISA Kit for Homo sapiens (Human)



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