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ACLY

ACLY,即ATP檸檬酸裂合酶,別名包括ATP-citratelyase、ACL、ATPCL、CLATP等。ACLY由人類ACLY基因編碼,該基因位于17號染色體的q21.2區域。ACLY是一種關鍵的代謝酶,催化ATP依賴的檸檬酸和輔酶A(CoA)轉化為草酰乙酸和乙酰輔酶A(Acetyl-CoA)的反應。乙酰輔酶A是脂肪酸代謝、膽固醇生物合成以及蛋白質乙酰化和異戊二烯化修飾的關鍵前體。在生物學意義上,ACLY通過連接糖代謝和脂肪酸合成,對細胞的代謝調控和能量平衡具有重要作用。ACLY在多種類型的癌癥中表達上調,其抑制能夠誘導癌細胞增殖停滯,因此被認為是癌癥治療的潛在靶點。

熱銷產品

Recombinant Human ATP-citRate synthase (ACLY), partial (CSB-EP001158HU)

驗證數據

CSB-EP001158HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ACLY Recombinant Monoclonal Antibody (CSB-RA712206A0HU)

驗證數據

CSB-RA712206A0HU

Western Blot
Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate
All lanes: ACLY antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 121, 120, 92 kDa
Observed band size: 120 kDa

CSB-RA712206A0HU

Immunofluorescence staining of HepG2 Cells with CSB-RA712206A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CSB-RA712206A0HU

Overlay histogram showing Hela cells stained with CSB-RA712206A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ACLY Antibodies

ACLY for Homo sapiens (Human)

ACLY Proteins

ACLY Proteins for Homo sapiens (Human)



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