Dog arginine vasopressin (AVP) ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬抗利尿激素/血管加壓素/精氨酸加壓素 (AVP)酶聯(lián)免疫試劑盒（CSB-EL002466DO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的AVP含量。AVP即精氨酸加壓素，在人體水鹽平衡、血壓調(diào)節(jié)、垂體激素釋放等生理過(guò)程中發(fā)揮重要作用。其研究機(jī)制主要圍繞AVP受體展開(kāi)，探索通過(guò)調(diào)節(jié)受體活性來(lái)干預(yù)相關(guān)疾病，如心力衰竭、多尿癥等，為開(kāi)發(fā)針對(duì)性藥物提供方向。試劑盒檢測(cè)范圍為0.78 pg/mL-50 pg/mL，適用于科研領(lǐng)域探究犬類(lèi)內(nèi)分泌系統(tǒng)功能、心血管疾病模型、脫水或水腫相關(guān)代謝研究，以及藥理學(xué)實(shí)驗(yàn)中藥物對(duì)體液調(diào)節(jié)通路的影響評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

AVP
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類(lèi)型：

serum, plasma
檢測(cè)范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.2 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	96
	Range %	92-101
1:4	Average %	86
	Range %	81-92
1:8	Average %	90
	Range %	84-97

回收率：

The recovery of dog AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	107	103-111

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.