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  4. Human glypican 1 (GPC1) ELISA kit

Human glypican 1 (GPC1) ELISA kit

  • 中文名稱：
    人磷脂酰肌醇蛋白聚糖1(GPC1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL009703HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人磷脂酰肌醇蛋白聚糖1(GPC1)酶聯(lián)免疫試劑盒（CSB-EL009703HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿.樣本中的GPC1含量。GPC1 即磷脂酰肌醇蛋白聚糖 -1，是細(xì)胞表面硫酸乙酰肝素蛋白聚糖家族成員。其過(guò)度表達(dá)與腫瘤發(fā)生發(fā)展相關(guān)。研究機(jī)制上，它參與腫瘤細(xì)胞增殖、遷移及血管生成等過(guò)程，還會(huì)影響腫瘤微環(huán)境，有望成為腫瘤診斷和治療的潛在靶點(diǎn)。試劑盒檢測(cè)范圍為0.156 ng/ml-10 ng/ml.，為科研人員探索GPC1在腫瘤生物學(xué)、干細(xì)胞分化或免疫調(diào)節(jié)等領(lǐng)域的功能提供可靠數(shù)據(jù)。該產(chǎn)品嚴(yán)格適用于基礎(chǔ)研究場(chǎng)景，例如通過(guò)檢測(cè)不同處理?xiàng)l件下細(xì)胞或動(dòng)物模型樣本中的GPC1動(dòng)態(tài)變化，解析其與病理生理過(guò)程的相關(guān)性，或作為潛在生物標(biāo)志物的篩選工具，為后續(xù)分子機(jī)制研究奠定基礎(chǔ)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    FLJ38078 ELISA Kit; glypican ELISA Kit; Glypican proteoglycan 1 ELISA Kit; glypican1 ELISA Kit; GPC 1 ELISA Kit; Gpc1 ELISA Kit; GPC1_HUMAN ELISA Kit; Secreted glypican-1 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates.
  • 檢測(cè)范圍：
    0.156 ng/ml-10 ng/ml.
  • 靈敏度：
    0.039 ng/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GPC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    90-100

    1:2

    Average %

    89

    Range %

    85-93

    1:4

    Average %

    92

    Range %

    88-96

    1:8

    Average %

    85

    Range %

    80-90

  • 回收率：

    The recovery of human GPC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    103

    99-109

    EDTA plasma (n=4)

    91

    86-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.267

    2.127

    2.197

    2.064

    5

    1.786

    1.875

    1.831

    1.698

    2.5

    1.295

    1.342

    1.319

    1.186

    1.25

    0.782

    0.846

    0.814

    0.681

    0.625

    0.516

    0.506

    0.511

    0.378

    0.312

    0.368

    0.352

    0.360

    0.227

    0.156

    0.273

    0.285

    0.279

    0.146

    0

    0.135

    0.131

    0.133

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
  • 基因功能參考文獻(xiàn)：
    1. High GPC1 expression is associated with uterine cervical cancer. PMID: 29055044
    2. The results demonstrated that the GPC1 gene was re-expressed in pancreatic ductal adenocarcinoma (PDAC) mainly due to promoter hypomethylation, even for early-stage PDAC. High levels of GPC1 were associated with poorer pathological differentiation and worse biological behaviors. GPC1 could serve as an independent unfavorable prognostic factor in PDAC. PMID: 28440066
    3. Glypican-1 was validated as a novel substrate for ADAM17, with important function in adhesion, proliferation and migration of carcinoma cells. PMID: 27576135
    4. Exosomal miR signature is superior to exosomal GPC1 or plasma CA 19-9 levels in establishing a diagnosis of pancreatic ductal carcinoma and differentiating between PDAC and chronic pancreatitis. PMID: 28232049
    5. The role of glypican-1 in mediating the eNOS activation under shear stress might involve in protecting the endothelial function against disturbed flow. PMID: 27688027
    6. independent case-control study implicated that common SNPs in GPC1 gene contributed to biliary atresia susceptibility in Chinese population PMID: 27373597
    7. The C terminus is shown to be highly flexible in solution, but it orients the core protein transverse to the membrane, directing a surface evolutionarily conserved in Gpc1 orthologs toward the membrane, where it may interact with signaling molecules PMID: 26203194
    8. differences in expression among different subtypes of ameloblastomas PMID: 25046223
    9. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease in patients with early-and late-stage pancreatic cancer. PMID: 26106858
    10. GPC1 and FGFR1 are targets for regulation of their gene expression by miR-149. PMID: 24463821
    11. DNA from 4 HPV positive and 4 HPV negative fresh frozen primary HNSCC were subject to comprehensive genome-wide methylation profiling.Pathway analysis of 1168 methylated genes showed 8 signal transduction pathways (GPC1) of which 62% are hypermethylated. PMID: 23736812
    12. Crystal structure of N-glycosylated human glypican-1 core protein shows the structure of two loops evolutionarily conserved in vertebrate glypican-1 PMID: 22351761
    13. High glucose can decrease the expression of core protein Sydecan-1 and Glypican-1 in cultured human renal glomerular endothelial cells. PMID: 21265098
    14. Mutagenesis and enzymatic cleavage indicated that the potential N-glycosylation sites are invariably occupied. PMID: 21932778
    15. The N-unsubstituted glucosamine residues are formed during biosynthesis of glypican-1 and the content increased upon inhibition of polyamine synthesis. PMID: 19479373
    16. Augmented synthesis and differential localization of heparan sulfate proteoglycans in Duchenne muscular dystrophy. PMID: 11968010
    17. S-nitrosylation and Copper-dependent autocleavage of glypican 1 PMID: 12084716
    18. inhibition of Gpc-1 expression abrogates spermine uptake and intracellular delivery; ascorbate released NO, which degraded heparan sulfate side chains and liberated the bound spermine PMID: 12972423
    19. glypican-1 is required for efficient TGF-beta1 signaling in pancreatic cancer cells PMID: 15249209
    20. syndecan-1 and glypican-1 have roles in progression of ovarian cancer PMID: 15297422
    21. glypican 1 (GPC1) is deleted in both reported patients suffering from Albright hereditary osteodystrophy-like (AHO-like) syndrome PMID: 15547662
    22. Enhanced GPC1 expression correlates with BMP and activin receptors in pancreatic cancer. PMID: 17016645
    23. cancer cell- and host-derived GPC1 are crucial for full mitogenic, angiogenic, and metastatic potential of cancer cells PMID: 18064304
    24. Glypican-3 can be especially useful in the identification of poorly differentiated hepatocellular carcinoma. PMID: 18536657
    25. study concludes that Abeta can modulate the cellular expression of agrin and glypican-1, which may contribute to the accumulation of these heparan sulfate proteoglycans in Alzheimer's disease lesions PMID: 19166823

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  • 相關(guān)疾病：
    Associates (via the heparan sulfate side chains) with fibrillar APP amyloid-beta peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain (PubMed:15084524).
  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.; [Secreted glypican-1]: Secreted, extracellular space.
  • 蛋白家族：
    Glypican family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4449

    OMIM: 600395

    KEGG: hsa:2817

    STRING: 9606.ENSP00000264039

    UniGene: Hs.328232



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