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  4. Crotonyl-HIST1H2AG (K118) Antibody

Crotonyl-HIST1H2AG (K118) Antibody

  • 中文名稱：
    Crotonyl-HIST1H2AG (K118)兔多克隆抗體
  • 貨號：
    CSB-PA010389PA118crHU
  • 規格：
    ￥660
  • 圖片：
    • Immunofluorescence staining of Hela cells with CSB-PA010389PA118crHU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-HIST1H2AG (CSB-PA010389PA118crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
    • Immunocytochemistry analysis of CSB-PA010389PA118crHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate (treated by 30mM sodium crotonylate for 4h)
      All lanes: HIST1H2AG antibody at 0.3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 15 kDa
      Observed band size: 15 kDa
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) HIST1H2AG Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    HIST1H2AG
  • 別名：
    H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Peptide sequence around site of Crotonyl-Lys (118) derived from Human Histone H2A type 1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, ICC, IF, ChIP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:200-1:2000
    ICC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2A family
  • 數據庫鏈接：

    HGNC: 4737

    OMIM: 602787

    KEGG: hsa:8329

    STRING: 9606.ENSP00000352627

    UniGene: Hs.134999



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