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Recombinant Staphylococcus aureus Histidine protein kinase saeS (saeS)

  • 中文名稱:
    金黃色釀膿葡萄球菌saeS重組蛋白
  • 貨號(hào):
    CSB-CF643036FLF
  • 規(guī)格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產(chǎn)品詳情

  • 基因名:
    saeS
  • Uniprot No.:
  • 別名:
    saeS; SAOUHSC_00714; Histidine protein kinase SaeS; Sensor protein SaeS; Staphylococcus exoprotein expression protein S
  • 種屬:
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白長度:
    full length protein
  • 表達(dá)區(qū)域:
    1-351
  • 氨基酸序列
    MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFI NPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQ QEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDM THIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCNEIDAFYQYRTPLE RILTNLLDNALKFSNVGSRIDINISENEDQDTIDIAISDEGIGIIPELQERIFERTFRVE NSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Member of the two-component regulatory system SaeR/SaeS involved in the regulation of staphylococcal virulence factors in a strain-dependent fashion. Probably functions as a membrane-associated protein kinase that upon sensing the appropriate signal, autophosphorylates and in turn activates the cytosolic response regulator SaeR. SaeR/SaeS activates the expression of exoproteins involved in adhesion and invasion of host cells, including hemolysins (hla, hlb), coa, Dnase, spa and cell wall-associated proteins (emp, eap, fnbA). Acts probably downstream of the agr system in the regulatory cascade of virulence factors.
  • 基因功能參考文獻(xiàn):
    1. the crystal structure of the DNA-binding domain of SaeR (SaeR(DBD)) was solved at 2.5 A resolution. The structure reveals that SaeR(DBD) exists as a monomer and has the canonical winged helix-turn-helix module. [SaeR] PMID: 26249357
    2. Our results indicate that the function of the linker peptide in SaeS is a highly evolved feature with very optimized amino acid sequences PMID: 25849574
    3. The SaeS protein is the histidine kinase responsible for phosphorylation of the response regulator SaeR. RNA-Seq results indicated that sae regulates many genes that may affect biofilm formation PMID: 25853849
  • 亞細(xì)胞定位:
    Cell membrane; Multi-pass membrane protein.
  • 數(shù)據(jù)庫鏈接:


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