Recombinant Rat Dynamin-like 120 kDa protein, mitochondrial (Opa1)

in vitro E.coli expression system

中文名稱：

大鼠Opa1重組蛋白
貨號：

CSB-CF651905RA
規(guī)格：
來源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Opa1
Uniprot No.：

Q2TA68
別名：

Opa1; Dynamin-like 120 kDa protein, mitochondrial; Optic atrophy protein 1 homolog
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
表達(dá)區(qū)域：

195-960
氨基酸序列

ATDHGSESDKHYRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKG IHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIF PRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEG CTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIIL CIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMK ALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWK MVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPK HWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAW ETLQDEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRS ISDKQQWDAAIYFMEEALQGRLKDTENAIENMIGPDWKKRWIYWKNRTQEQCVHNETKNE LEKMLKVNDEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCN LCRRGFYYYQRHFIDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVL EDFAEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes. Plays a role in remodeling cristae and the release of cytochrome c during apoptosis. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Plays a role in mitochondrial genome maintenance.; Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.; Isoforms that contain the alternative exon 4b (present in isoform 2 and isoform 3, but not in isoform 1) are required for mitochondrial genome maintenance, possibly by anchoring the mitochondrial nucleoids to the inner mitochondrial membrane.
基因功能參考文獻(xiàn)：
1. evidences have been obtained that ROS-dependent apoptosis is associated with an alteration of mitochondrial cAMP/PKA signal that causes degradation/proteolysis of Sirt3 that, in turn, promotes acetylation and proteolytic processing of OPA1 PMID: 27890624
2. Retinal ischemia-reperfusion (I/R) or hypoxia-reoxygenation (H/R) injury induces Opa1 long isoforms loss. PMID: 26530815
3. Data indicate that the expression of the dynamin-related GTPase OPA1 increased significantly in the pretreated groups, especially in the pre-exercised ischemia group compared with the non-treated groups. PMID: 24633199
4. Chronic muscle use increases the ratio of opa1 leading to reticular mitochondria, whereas muscle disuse and aging result in a decrease in this ratio, culminating in fragmented mitochondria. PMID: 23494933
5. data suggest that OPA1 cleavage is a likely convergence point for mitochondrial dysfunction and imbalances in mitochondrial fission and fusion induced by oxidative or nitrosative stress. PMID: 23220553
6. OPA1 plays a role in synaptic maturation and dendritic growth through mitochondrial dynamics in neuronal functioning. PMID: 23543485
7. These findings suggest that mitochondrial preservation after inhibition of NOS-2 may be useful for protecting retinal ganglion cells against glaucomatous damage. PMID: 21220562
8. The data suggest an important and specific function of the OPA1 protein, not only in the optic nerve forming ganglion cells but also in the intrinsic signal processing of the inner retina. PMID: 15505078
9. OPA1 is predominantly expressed in retinal ganglion cells of the normal rat retina and axons of the optic nerve. These findings may explain the selective vulnerability of retinal ganglion cells to OPA1 loss of function. PMID: 15912498
10. Mammalian mitochondrial function and morphology is regulated through processing of OPA1 in a DeltaPsi-dependent manner. PMID: 16778770
11. expression analyses of OPA1 in the rat auditory and vestibular organ PMID: 17828551
12. data are the first to demonstrate OPA1 cleavage during neuronal apoptosis and they implicate caspases as indirect regulators of OPA1 processing in degenerating neurons PMID: 19046944
13. These results indicate that OPA1 release from mitochondria triggered by acute intraocular pressure elevation is inhibited by blockade of glutamate receptor activation. PMID: 19122832
14. Apoptotic cell death via reduction of OPA1 and mitochondrial fusion may contribute to heart failure progression. PMID: 19493956
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亞細(xì)胞定位：

Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space. Mitochondrion membrane.
蛋白家族：

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
組織特異性：

Expressed in brain as well as retinal ganglion, starbust amacrine and horizontal cells of the retina. Absent from nerve fibers and photoreceptor cells of the retina.
數(shù)據(jù)庫鏈接：

KEGG: rno:171116

UniGene: Rn.225901