Recombinant Mouse Fatty-acid amide hydrolase 1 (Faah)

in vitro E.coli expression system

中文名稱：

小鼠Faah重組蛋白
貨號：

CSB-CF007938MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Faah
Uniprot No.：

O08914
別名：

Faah; Faah1; Fatty-acid amide hydrolase 1; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-579
氨基酸序列

MVLSEVWTALSGLSGVCLACSLLSAAVVLRWTRSQTARGAVTRARQKQRAGLETMDKAVQRFRLQNPDLDSEALLALPLLQLVQKLQSGELSPEAVLFTYLGKAWEVNKGTNCVTSYLTDCETQLSQAPRQGLLYGVPVSLKECFSYKGHASTLGLSLNEGVTSESDCVVVQVLKLQGAVPFVHTNVPQSMLSYDCSNPLFGQTMNPWKPSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSAFCGICGLKPTGNRLSKSGLKSCVYGQTAVQLSVGPMARDVDSLALCMKALLCEDLFRLDSTIPPLPFREEIYRSSRPLRVGYYETDNYTMPTPAMRRAVMETKQSLEAAGHTLVPFLPNNIPYALEVLSAGGLFSDGGCSFLQNFKGDFVDPCLGDLVLVLKLPRWFKKLLSFLLKPLFPRLAAFLNSMCPRSAEKLWELQHEIEMYRQSVIAQWKAMNLDVVLTPMLGPALDLNTPGRATGAISYTVLYNCLDFPAGVVPVTTVTAEDDAQMEHYKGYFGDMWDNILKKGMKKGIGLPVAVQCVALPWQEELCLRFMREVERLMTPEKRPS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the hydrolysis of endogenous amidated lipids like the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides such as the taurine-conjugated fatty acids (a structural class of central nervous system (CNS) metabolites), to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol).
基因功能參考文獻：
1. These data demonstrate that FAAH activity is required for leptin's hypophagic effects PMID: 29967158
2. Dual FAAH and TRPV1 blockage inhibits contextual fear memory. PMID: 28583049
3. Results suggest that fatty acid amide hydrolase (FAAH)-regulated N-acyl-taurines (NATs) signaling as a lipid-based mechanism of wound-healing control in mammalian skin, which might be targeted for chronic wound therapy. PMID: 27412859
4. Both inhibitors reduced several markers of macrophage activation, such as mRNA expression of inflammatory mediators, as well as cytokine and prostaglandin production, with however some differences between FAAH and NAAA inhibition. Our results support an important role for inhibition of NAE hydrolysis and NAAA inhibition in particular in controlling macrophage activation, and thus inflammation. PMID: 28065729
5. Inactivation of FAAH, the main degrading enzyme of anandamide and similar endocannabinoids, could lead to an increased decidual endocannabinoid tone with embryotoxic effects. PMID: 27731508
6. genetic deletion of FAAH may predispose animals to increased sensitivity to certain types of pain. PMID: 27178246
7. Impaired neurogenesis by HIV-1-Gp120 is rescued by genetic deletion of fatty acid amide hydrolase enzyme PMID: 24571443
8. Study identified FAAH as a novel player in the pathogenesis of lupus PMID: 26773143
9. Basal concentrations of anandamide was greater, and the severity of cystitis was reduced FAAH KO mice. Cystitis-associated increased peripheral sensitivity and enhanced bladder activity were attenuated in FAAH KO mice. PMID: 25374388
10. in FAAH(-/-) animals the number of microglia and the ratio of activated microglia and IL-1beta level were already higher in young animals PMID: 25534441
11. Results demonstrate that the supra-spinally-located FAAH enzyme is necessary for the analgesic action of paracetamol. PMID: 25448494
12. Data suggest that multitarget FAAH/Cox blockade may provide a transformative approach to inflammatory bowel disease (IBD) and other pathologies in which fatty acid amide hydrolase/cyclooxygenases (FAAH, Cox-1, and Cox-2) are overactive. PMID: 25757568
13. our results do not support a clear role of FAAH, CNR1 and NAPE-PLD in BD and lithium response. PMID: 24126189
14. FLAT does not serve as a global intracellular AEA carrier PMID: 24223930
15. FAAH inhibitors provide a new class of anti-spastic agents that may have utility in treating spasticity in multiple sclerosis. PMID: 23625705
16. FAAH is required for chronic stress to induce hyperactivity and structural amygdala remodeling. FAAH-mediated decreases in arachidonylethanolamine signaling after chronic stress. This loss is functionally relevant to the effects of chronic stress. PMID: 22776900
17. The endotoxin induced decrease in peripheral blood mononuclear cell FAAH activity is reversed by progesterone in a receptor-mediated fashion. PMID: 23906535
18. Data suggest that FAAH structure is modulated by membranes; FAAH preferentially binds to membranes containing both endocannabinoid/anandamide (AEA) and cholesterol; cholesterol modulates FAAH activity; FAAH co-localizes with AEA and cholesterol. PMID: 24215562
19. Suggest that organophosphate agents induce plasma hypertriglyceridemia in mouse through single or dual inhibition of FAAH or/and MAGL, apparently leading to overstimulation of cannabinoid signal regulating energy metabolism. PMID: 24361246
20. Pulmonary hypertension is prevented in FAAH-/- mice or by treating wild-type mice with a FAAH antagonist for 3 wk of hypoxia. PMID: 24167249
21. FAAH but not monoacyl glycerol lipase exerts important protective actions against 2-arachidonoyl glycerol-induced cellular damage. PMID: 23806692
22. FAAH deficiency is associated with the development of smaller atherosclerotic plaques with high neutrophil content, accompanied by an increased proinflammatory immune response. PMID: 23241405
23. The faah gene is a direct target of estrogen in the testis. PMID: 22802127
24. FAAH deletion, and the resulting increases in NAEs(anandamide and palmitoylethanolamide), predispose mice to ectopic lipid storage and hepatic insulin resistance by promoting centrally mediated hypothyroidism. PMID: 22912404
25. This review presented that the FAAH knockout mice show a correlation between 5-HT firing rate and PMID: 23089640
26. Data suggest that deletion of FAAH in astrocytes exacerbates inflammatory response to beta-amyloid in process involving peroxisome proliferator-activated receptor (PPAR) alpha, PPAR-gamma, and TRPV1 (transient receptor potential cation channel V1). PMID: 22321194
27. Blockade of neuronal FAAH reverses allodynia through the activation of both cannabinoid receptors and represents a promising target to treat inflammatory pain PMID: 21506952
28. Peripheral effects of FAAH deficiency on fuel and energy homeostasis: role of dysregulated lysine acetylation. PMID: 22442717
29. Messenger RNA and protein for FAAH was expressed in the mucosa of mouse bladder. PMID: 21930339
30. overexpression of fatty acid amide hydrolase shortens the duration of depolarization-induced suppression of excitation of synaptic transmission in cultured mouse hippocampal neurons. PMID: 21564090
31. Dual inhibition of alpha/beta-hydrolase domain 6 and fatty acid amide hydrolase increases endocannabinoid levels in neurons. PMID: 21665953
32. Results support the role of FAAH in the regulation of emotional reactivity and suggest that anandamide-mediated hyperactivation of CB1 is responsible for the emotional phenotype of FAAH(-/-) mice and for their enhanced serotonergic tone. PMID: 21042794
33. In pathological conditions associated with acute oxidative/nitrative stress FAAH plays a key role in controlling the tissue injury that is, at least in part, mediated by the activation of CB(1) receptors by endocannabinoids. PMID: 21070851
34. The result of this study suggested that FAAH genetic deletion enhances anxiolytic-like and antidepressant-like effects, paralleled by altered 5-HT transmission and postsynaptic 5-HT(1A) and 5-HT(2A/2C) receptor function. PMID: 20571484
35. This study showed that FAAH-/- mice display differential tolerance, dependence, and cannabinoid receptor adaptation after delta 9-tetrahydrocannabinol and anandamide administration. PMID: 20357755
36. oleamide shows an anti-inflammatory effect through inhibition of NF-kappaB activation PMID: 20298753
37. here FAAH may be involved in regulation of signaling mediated by other endocannabinoid receptors or by receptors for other fatty acid amide signaling molecules. PMID: 12770562
38. These findings support the role of fatty acid amides as possible modulators of sleep and indicate that the homeostatic mechanisms of sleep in FAAH (-/-) mice are not disrupted. PMID: 15453543
39. Mice lacking FAAH have normal hemodynamic profile. Increased responsiveness to anandamide-induced hypotension and cardiodepression is due to decreased degradation of anandamide rather than increased target organ sensitivity to CB1 agonists. PMID: 15821037
40. FAAH is a physiologic regulator of intestinal motility and a potential target for the development of drugs capable of reducing intestinal motility. PMID: 16143133
41. FAAH and GSH are determinants of anandamide-mediated cell death in the liver PMID: 16418162
42. FAAH is a key metabolic gatekeeper, regulating on-site anandamide tone to direct preimplantation events that determine the fate of pregnancy. PMID: 16886060
43. Multiple anandamide (AEA)-induced metabolites were observed in brains from FAAH(-/-) mice, including a major product with a mass shift of +165 Da (m/z 513). The structure of this product was determined to be O-phosphorylcholine (PC)-AEA. PMID: 16981687
44. FAAH inhibition represents a promising pharmacological approach to treat psychopathologies hallmarked by an inability to extinguish maladaptive behaviors, such as post-traumatic stress syndrome and obsessive-compulsive disorder. PMID: 17047668
45. FAAH null mutant mice showed higher preference for alcohol and voluntarily consumed more alcohol than wild-type littermates. PMID: 17164820
46. Mice lacking FAAH are more resistant to age-associated decline in cardiac function that wild type littermates. PMID: 17434980
47. FAAH(-/-) mice showed reduced anxiety both in the elevated plus maze and in the light-dark test. PMID: 17709120
48. Genetic loss of Faah results in elevated levels of anandamide, an endocannabinoid, in the male reproductive system, leading to compromised fertilizing capacity of sperm. This defect is rescued by superimposing deletion of cannabinoid receptor 1 (Cnr1). PMID: 18987328
49. Biochemical and biological properties of 4-(3-phenyl-[1,2,4] thiadiazol-5-yl)-piperazine-1-carboxylic acid phenylamide, a mechanism-based inhibitor of fatty acid amide hydrolase. PMID: 19095868
50. FAAH (-/-) mice displayed accelerated acquisition rates in an aversively-motivated, but not in the appetitively-motivated, Barnes maze task. PMID: 19524055
顯示更多

收起更多
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.
蛋白家族：

Amidase family
數據庫鏈接：

KEGG: mmu:14073

STRING: 10090.ENSMUSP00000041543

UniGene: Mm.256025