Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5)-VLPs

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5)-VLPs
貨號：

CSB-MP664660MO
規格：
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Marchf5
Uniprot No.：

Q3KNM2
別名：

Membrane-associated RING finger protein 5;Membrane-associated RING-CH protein V MARCH-V;;RING-type E3 ubiquitin transferase MARCHF5
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

Mammalian cell
表達區域：

1-278aa
氨基酸序列

MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it's possible to develop.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from PBS, 6% Trehalose, pH 7.4.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
基因功能參考文獻：
1. These findings suggest that MITOL plays a protective role against apoptosis in cardiomyocytes. PMID: 27444773
2. MARCH5 binds MAVS only during viral stimulation when MAVS forms aggregates, and these interactions require the RING domain of MARCH5 and the CARD domain of MAVS. PMID: 26246171
3. March5 is involved in maintaining mouse-ESC (mESC) pluripotency via catalysis of Prkar1a polyubiquitination and inhibition of the Raf/MEK/ERK pathway. PMID: 26033541
4. A fine balance of Mfn1 levels is maintained by MARCH5-mediated quality control on acetylated Mfn1. PMID: 24722297
5. MARCH5 is an authentic E3 ubiquitin ligase and catalyzes K63-linked polyubiquitination of TANK. MARCH5 modulates TLR7 signaling via releasing the inhibitory action of TANK toward TRAF6. PMID: 21625535
6. Results indicate that MITOL plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway. PMID: 19741096
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亞細胞定位：

Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
數據庫鏈接：

KEGG: mmu:69104

STRING: 10090.ENSMUSP00000024078

UniGene: Mm.175989