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Recombinant Arabidopsis thaliana Ethylene receptor 1 (ETR1)

  • 中文名稱:
    擬南芥ETR1重組蛋白
  • 貨號:
    CSB-CF344583DOA
  • 規格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產品詳情

  • 基因名:
    ETR1
  • Uniprot No.:
  • 別名:
    ETR1; At1g66340; T27F4.9; Ethylene receptor 1; AtETR1; Protein ETHYLENE RESPONSE 1; Protein ETR1
  • 種屬:
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-738
  • 氨基酸序列
    MEVCNCIEPQWPADELLMKYQYISDFFIAIAYFSIPLELIYFVKKSAVFPYRWVLVQFGA FIVLCGATHLINLWTFTTHSRTVALVMTTAKVLTAVVSCATALMLVHIIPDLLSVKTREL FLKNKAAELDREMGLIRTQEETGRHVRMLTHEIRSTLDRHTILKTTLVELGRTLALEECA LWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG KYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA DQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH AIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH TLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI SVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS SGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA IPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV CMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN IRDVLSDLLEPRVLYEGM
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling. In the presence of ethylene, the auto-kinase activity of ETR1 is inhibited and the non-phosphorylated kinase domain binds tightly to the corresponding domain of EIN2.
  • 基因功能參考文獻:
    1. ETR1-receiver domain already exists as an active formation and executes its function through binding with the downstream factors without phosphorylation. PMID: 27486797
    2. The ETR1 receiver domain has multiple functions where different surfaces are involved in the control of different traits. PMID: 26160962
    3. Data show that the cytosolic domain of ETR1 plays a key role, interacting with all other receptors and several participants of the ethylene signaling pathway. PMID: 25451923
    4. Data show that Bacillus sp B55, which associated with Nicotiana attenuata roots, promotes growth of wild-type and, particularly, ethylene (ET)-insensitive (35)S-ethylene response1 (etr1) plants expressing the mutant Arabidopsis thaliana receptor ETR1-1. PMID: 23903320
    5. Histidine kinase activity of ETR1 is not required for but plays a modulating role in the regulation of ethylene responses. PMID: 22467798
    6. Transgenic and genetic analyses revealed that this organelle abnormality was not linked to etr1-1 or ethylene signaling, but linked to a second mutation in ARC3, which was further verified by genetic complementation analysis. PMID: 22228186
    7. A mutation in the kinase domain of ETR1 that prevents auto-phosphorylation (H353A), increases the affinity of the receptors for EIN2. PMID: 20591837
    8. Impaired perception of ethylene via ETR1 results in increased disease resistance to Verticillium dahliae. PMID: 20447269
    9. Induction of etr1-1 expression repressed the epinastic response to ethylene as well as delayed fruit ripening. Restoration of ethylene sensitivity was achieved following the cessation of the induction PMID: 20181754
    10. Inhibition of autophosphorylation in ETR1 by cyanide in Arabidopsis thaliana can be controlled by etehylene or ethylene agonists. PMID: 19825547
    11. histidine kinase activity is not necessary for ethylene receptor function in plants and its signal transduction does not occur through a phosphorelay mechanism PMID: 15358768
    12. The His kinase domain in the C-terminal region of Arabidopsis ETR1 plays a role in the repression of ethylene responses. PMID: 15466228
    13. Five receptor isoforms from Arabidopsis thaliana confirmed that all members of the family are capable of high-affinity ethylene-binding activity. PMID: 15703053
    14. Data demonstrate an unexpected role for ETR1, that of mediating stomatal closure in response to H2O2. PMID: 15761208
    15. These findings demonstrate that RTE1 is a negative regulator of ethylene signaling and suggest that RTE1 plays an important role in ETR1 function. PMID: 16682642
    16. Possible roles of subfamily I receptors and disulfide linkages in ETR1 receptor signal output mediated through the N terminus are discussed. PMID: 16891553
    17. The ETR1 receptor is required for ethylene-stimulated nutations. PMID: 17071649
    18. These findings yield insight into the structure and function of the ethylene binding domain (EBD) and suggest a conserved role of the EBD as a negative regulator of the signal transmitter domain. PMID: 17189345
    19. The role of the histidine kinase activity of ETR1 in A. thaliana growth and development is reported. PMID: 17284582
    20. ETR1 is positively regulated by RTE1 via the ethylene-binding domain, independently from RAN1 activity. PMID: 18643990

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  • 亞細胞定位:
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Ethylene receptor family
  • 組織特異性:
    Leaves, roots, stems, seedlings, flowers, anthers, carpels and ovules.
  • 數據庫鏈接:

    KEGG: ath:AT1G66340

    STRING: 3702.AT1G66340.1

    UniGene: At.43238



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