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V5-Tag Monoclonal Antibody

  • 中文名稱:
    V5-Tag鼠單克隆抗體
  • 貨號:
    CSB-MA000161M1m
  • 規(guī)格:
    ¥400
  • 圖片:
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa
      Observed band size: 32, 21, 55, 28, 58, 33 KDa
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: 50ng V5-tagged fusion protein
      V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000
      Lane 1: Recombinant V5-tagged fusion protein at 25ng
      Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
      Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
      Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
      Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 32 kd
      Observed band size: 32 kd
      Exposure time: 5min
    • Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
      Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate
      Lane 2: CSB-MA000161M1m (2.5μl) + Transfected HEK293F cells whole cell lysate (500μg)
      Lane 3: Transfected HEK293F cells whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    CUSABIO貨號:CSB-MA000161M1m V5-Tag單克隆抗體是一款針對V5表位標(biāo)簽設(shè)計的高特異性科研試劑,適用于檢測重組蛋白中的V5標(biāo)簽。V5-Tag作為分子生物學(xué)研究中常用的短肽標(biāo)簽(14個氨基酸),可特異性標(biāo)記重組蛋白以便于追蹤、純化和功能研究。本產(chǎn)品經(jīng)嚴(yán)格驗證具有卓越的物種廣譜性,可在ELISA實驗中實現(xiàn)精準(zhǔn)定量檢測,通過Western Bloting(WB)清晰呈現(xiàn)目標(biāo)條帶,并支持免疫熒光(IF)進行亞細(xì)胞定位分析,同時適用于免疫共沉淀(IP)實驗以研究蛋白質(zhì)相互作用。抗體采用雜交瘤技術(shù)制備,批次穩(wěn)定性優(yōu)異,可滿足基因表達調(diào)控、蛋白質(zhì)相互作用網(wǎng)絡(luò)構(gòu)建、轉(zhuǎn)基因生物模型驗證等科研需求。該產(chǎn)品兼容哺乳動物、昆蟲等多種表達系統(tǒng),是研究重組蛋白表達效率、亞細(xì)胞定位及功能機制的理想工具,廣泛應(yīng)用于分子生物學(xué)、細(xì)胞生物學(xué)及蛋白質(zhì)組學(xué)領(lǐng)域的基礎(chǔ)研究。
  • 基因名:
    V5-Tag
  • 宿主:
    Mouse
  • 反應(yīng)種屬:
    All
  • 免疫原:
    GKPIPNPLLGLDST synthetic peptide conjugate to KLH
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG2a
  • 純化方式:
    >95%, Protein A purified
  • 克隆號:
    9E8B7
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IF, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:5000-1:1280000
    IF 1:100-1:200
    IP 2μl-5μl
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價



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