在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

GFP Monoclonal Antibody

  • 中文名稱:
    GFP鼠單克隆抗體
  • 貨號:
    CSB-MA000051M1m
  • 規格:
    ¥400
  • 圖片:
    • Western Blot
      Positive WB detected in: 50ng recombinant protein
      All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time:5min

    • Western Blot
      Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng
      All lanes: GFP antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time:5min

    • Western Blot
      Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg
      All lanes GFP antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
      Observed band size: 32,32,50,32,32 KDa
      Exposure time:1min

    • Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).

    • Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.

    • Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.

    • Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP
      Lane 1: Mouse control IgG2b instead of CSB-MA000051M1m in 293F whole cell lysate transfected with GFP
      Lane 2: CSB-MA000051M1m (4μg) + 293F whole cell lysate transfected with GFP (500μg)
      Lane 3: 293F whole cell lysate transfected with GFP (5μg)
      For western blotting, the blot was detected with CSB-MA000051M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

  • 其他:

產品詳情

  • 產品描述:
    本產品為GFP單克隆抗體(CUSABIO貨號:CSB-MA000051M1m),專為檢測綠色熒光蛋白(GFP)設計。GFP作為分子生物學中廣泛應用的熒光標記蛋白,其特異性檢測在基因表達示蹤、蛋白定位分析及細胞功能研究中具有重要價值。該抗體通過ELISA、Western Blot(WB)、免疫熒光(IF)、流式細胞術(FC)和免疫沉淀(IP)五大實驗平臺驗證,適用于轉基因細胞株篩選、重組蛋白表達驗證、熒光融合蛋白追蹤等科研場景,展現廣泛的物種兼容性。其高靈敏度和特異性可精準識別天然GFP及其衍生變體(如EGFP、YFP),在活細胞成像、蛋白質相互作用研究及報告基因系統中表現卓越。作為科研級試劑,本產品采用高純度雜交瘤技術制備,批次穩定性優異,為熒光標記實驗提供可靠工具,助力基因編輯、細胞信號通路及亞細胞結構研究。核心關鍵詞:GFP單克隆抗體、綠色熒光蛋白檢測、免疫印跡抗體、流式細胞術試劑、免疫熒光探針、重組蛋白檢測。
  • 基因名:
    GFP
  • 別名:
    CFP, eGFP, eYFP, GFP, GFP tag, YFP
  • 宿主:
    Mouse
  • 反應種屬:
    N/A
  • 免疫原:
    Recombinant GFP Protein
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal Antibody
  • 抗體亞型:
    IgG2b
  • 純化方式:
    >95%,Protein A purified
  • 克隆號:
    6C11C11
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    liquid
  • 應用范圍:
    ELISA, WB, IF, FC, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:50000-1:6400000
    IF 1:50-1:200
    FC 1:100-1:300
    IP 1μl-2μl
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價



主站蜘蛛池模板: 无码人妻精品一区二区三 | 欧美亚洲另类 丝袜综合网| 日韩精品一卡2卡3卡4卡新区视频| 国产精品久久久久高潮| 巨爆乳无码视频在线观看| 97se亚洲国产综合在线| 日本狂喷奶水在线播放212| 亚洲欧美国产国产一区二区三区| 国产亚洲精品久久yy50| 欧美与黑人午夜性猛交久久久| 可以看三级的网站| 四虎永久免费地址入口| 蜜臀视频一区二区在线播放| 国产在线精品二区| 国产无套内射久久久国产| 亚洲精品卡2卡3卡4卡5卡区| 18禁美女黄网站色大片在线| 超碰97久久国产精品牛牛| 日产一区日产2区| 国产成人精品.视频| 国产欧色美视频综合二区| av无码免费永久在线观看| 欧美老人巨大xxxx做受视频| 久久综合伊人九色综合| 日韩精品少妇无码受不了| 国产freexxxx性播放麻豆| 女人被狂躁c到高潮视频| 欧洲一卡2卡3卡4卡国产| 99c视频色欲在线| 四川丰满妇女毛片四川话| 在线不卡日本v一区二区| 国产亚洲另类无码专区| 久久婷婷成人综合色综合| 50岁退休熟女露脸高潮| 十八禁无码精品a∨在线观看| 国产成_人_综合_亚洲_国产| 少妇人妻偷人精品视蜜桃| 国模无码人体一区二区| 国产午夜av秒播在线观看| 国产精品自产拍高潮在线观看| 在阳台上玩弄人妻的乳球|