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  4. Recombinant rRNA 2'-O-methyltransferase fibrillarin (fib-1)

Recombinant rRNA 2'-O-methyltransferase fibrillarin (fib-1)

  • 中文名稱：
    秀麗隱桿線蟲fib-1重組蛋白
  • 貨號：
    CSB-YP632535CXY
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲fib-1重組蛋白
  • 貨號：
    CSB-EP632535CXY
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲fib-1重組蛋白
  • 貨號：
    CSB-EP632535CXY-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲fib-1重組蛋白
  • 貨號：
    CSB-BP632535CXY
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲fib-1重組蛋白
  • 貨號：
    CSB-MP632535CXY
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fib-1
  • Uniprot No.：
  • 別名：
    fib-1; T01C3.7rRNA 2'-O-methyltransferase fibrillarin; EC 2.1.1.-; Histone-glutamine methyltransferase
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-352
  • 氨基酸序列
    MGRPEFNRGG GGGGFRGGRG GDRGGSRGGF GGGGRGGYGG GDRGSFGGGD RGGFRGGRGG GDRGGFRGGR GGGDRGGFGG RGSPRGGFGG RGSPRGGRGS PRGGRGGAGG MRGGKTVVVE PHRLGGVFIV KGKEDALATK NMVVGESVYG EKRVSVDDGA GSIEYRVWNP FRSKLAASIM GGLENTHIKP GTKLLYLGAA SGTTVSHCSD VVGPEGIVYA VEFSHRSGRD LLGVAKKRPN VVPIVEDARH PHKYRMLVGM VDVIFSDVAQ PDQARIVALN AQNFLRNGGH AVISIKANCI DSTAEPEAVF AGEVNKLKEE KFKPLEQVTL EPYERDHAVV VAVYRPVKGK KV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Plays a role in modulation of nucleolus size most likely through regulating the ribosomal RNA (rRNA) pool.
  • 基因功能參考文獻：
    1. Fibrillarin acts as a central node in a regulatory network engaged in imparting immunity against bacterial pathogens. PMID: 30190478
  • 亞細胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族：
    Methyltransferase superfamily, Fibrillarin family
  • 數據庫鏈接：

    KEGG: cel:CELE_T01C3.7

    STRING: 6239.T01C3.7.1

    UniGene: Cel.23500



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